The 45th Annual Fitz-Hugh Symposium - Resident Research Presentations - Abstracts

Sep 5, 2026

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Conference program and resident research abstract collection from the University of Virginia Department of Otolaryngology–Head & Neck Surgery at the 45th Annual Fitz-Hugh Symposium. Organized by Basic/Translational/Applied Science and Clinical Science, it compiles multiple studies spanning gene editing with CRISPR-based mouse models, experimental in vivo phonation modeling, outcomes for sinonasal disease treatments, machine learning and imaging biomarkers for head and neck cancer, social media analysis for Bell’s palsy, and clinical predictors in tumor and airway conditions. Includes objectives, designs, methods, results, and conclusions for each presentation.

LanguageEnglish

CategoryResearch & Report

FormatPDF

Pages7

Reading Time18 min

AccessFree to download

Basic/Translational/Applied Science

Using CRISPR to create an HA-tagged knock-in mouse to localize ATP8B1 in hair cell stereocilia subdomains

Novel in vivo leporine phonation model for glottic insufficiency

Effects of emu oil and statins on sinonasal symptoms and quality of life in refractory chronic rhinosinusitis

Bell’s palsy: seeking the answers in social media

Machine learning and radiographic biomarkers of head and neck cancer

An unsupervised analysis of type 1 laryngeal cleft phenotypes and outcomes

Clinical Science

Utility and outcomes of circumferential peripheral margins in head and neck melanoma excision

The effect of vestibular schwannoma growth rate on audiometric performance

Surgeon review of 4D CT

Predictors of laryngeal stenosis development and tracheostomy dependence after acute laryngeal injury

Predictors of false negative sentinel lymph node biopsy in clinically localized Merkel cell carcinoma

THE UNIVERSITY OF VIRGINIA DEPARTMENT OF OTOLARYNGOLOGY – HEAD & NECK SURGERY PRESENTS THE 45th ANNUAL FITZ-HUGH SYMPOSIUM RESIDENT RESEARCH PRESENTATIONS Friday June 18, 2021 at 8:30 am Pinn Hall Basic/Translational/Applied Science Using CRISPR to create an HA-tagged knock-in mouse to localize ATP8B 1 in the hair cell’s stereocilia subdomains ......................................................................................... Jonas (Shin) Novel in vivo leporine phonation model for glottic insufficiency ......................................... Churnin (Daniero) Effects of emu oil and statins on sinonasal symptoms and quality of life in patients with refractory chronic rhinosinusitis—research block update .............................................. Basu (Mattos) Bell’s palsy: seeking the answers in social media ................................................................ .............Iorio (Oyer) Machine learning and radiographic biomarkers of head and neck cancer, a collaborative approach......................................... ................................................................ ....Mella (Garneau) An unsupervised analysis of type 1 laryngeal cleft phenotypes and outcomes in children who undergo diagnostic laryngoscopy for severe respiratory symptoms ............... Saez (Early/Brand) [break] Clinical Science Utility and outcomes of circumferential peripheral margins in head and neck melanoma excision .............................................................................................. Jung (Jameson) The effect of vestibular schwannoma growth rate on audiometric performance ........................ Casale (Kesser) Surgeon review of 4D CT...........................................................................................................Barker (Shonka) Predictors of laryngeal stenosis development and tracheostomy dependence after acute laryngeal injury ................................................................................................ . Carpenter (McGarey) Predictors of false negative sentinel lymph node biopsy in clinically localized Merkel cell carcinoma.............................................................................................. Bartels (Jameson) | RESIDENT RESEARCH ABSTRACTS | | --- | | Using CRISPR to Create an HA-Tagged Knock-In Mouse to Localize ATP8B1 in the Hair Cell’s Stereocilia Subdomains Rachel Jonas MD; Jung-Bum Shin PhD
Objective: Use CRISPR to generate a mouse line in which an HA-tag is knocked into the endogenous Atp8b1 locus (HA-tag at the N-terminus) to localize ATP8B1 in the hair cell’s stereocilia subdomains using confocal microscopy.
Design: Animal research study
Methods: For generation of HA-ATP8B1-N mice we used the online tool CRISPOR to select suitable target sequences. Neonatal mouse pups were sacrificed by rapid decapitation. Genotyping was performed by PCR amplification of the region of interest. For immunofluorescence, the organs were fixed and rabbit anti-HA antibody was used followed by imaging with a scanning confocal microscope. Western blot was performed with both mouse anti-HA antibody and mouse anti-ATP8B1 antibody using the liver from HA-ATP8B1-N mice and wildtype mice. For generation of HA-ATP8B1-C mice we used the online tool CRISPOR to select suitable target sequences.
Results: An HA-ATP8B1-N mouse was successfully generated via CRISPR, with normal ABR ofa founder mouse. However, we were unable to localize the protein using confocal microscopy. A Western blot was performed, indicating that the HA tag had been cleaved from the ATP8B1 protein, which resulted in loss of immunoreactivity. Therefore, CRISPR was used to knock-in an HA tag to the C-terminus of the Atp8b1 locus.
Conclusions: We have yet to localize ATP8B1 in the hair cell’s stereocilia subdomains due to cleavage of the tagging peptide, but are hopeful that addition of the HA tag to the C-t

FAQs

What is the scope of the resident research abstracts in this symposium program?

The abstracts cover both Basic/Translational/Applied Science and Clinical Science topics, including experimental research methods and clinical outcome studies related to otolaryngology and head & neck conditions.

What kinds of research methods are represented in the abstracts?

Methods include gene-editing with CRISPR and imaging/genotyping workflows, experimental in vivo animal trials, social media analysis, machine learning approaches using radiographic data, and clinical predictors derived from patient outcomes.

How are the abstracts organized within the document?

They are grouped by scientific track: Basic/Translational/Applied Science first, followed by Clinical Science, with each presentation presented as an abstract containing objective, design/methods, results, and conclusions.

Sep 5, 2026

Upload by Kyle