Ptbp1 knockdown induces conversion of rat spinal cord astrocytes into neuron-like cells - Correction - Figure 8 and flow cytometry legend

Q: What was the main issue corrected in the original Figure 8?

The original Figure 8 contained an error where the upper four panels of 8E were inadvertently repeated in the lower four panels of 8B.

Q: What additional method information was missing for Figure 2A flow cytometry?

The Materials and methods description for the flow cytometry results of Figure 2A was omitted; it now includes the half-medium change schedule and the 48-hour GFP-lentivirus transduction with flow-cytometry quantification and GFP-positive gating based on blank control before analyzing experimental groups.

Q: How does SAG treatment relate to MAP2 expression in the corrected figure?

The corrected Figure 8 indicates that the Shh signaling pathway agonist SAG facilitates MAP2 expression in immature converted neuron-like cells, with corresponding immunofluorescence images and quantification comparing SAG-treated versus untreated groups.

Sep 30, 2026

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Correction notice addressing errors in the original Scientific Reports article on Ptbp1 knockdown and astrocyte-to-neuron-like conversion in rat spinal cord. It specifies that Figure 8 panels were incorrectly repeated, and it provides the corrected description of the original Figure 8 and associated legend. The correction also updates the Materials and methods section for the flow cytometry results of Figure 2A, adding the missing transduction efficiency quantification workflow and gating strategy. It includes figure-level details for markers and treatments.

LanguageEnglish

CategoryHealthcare

FormatPDF

Pages3

Reading Time8 min

AccessFree to download

Correction to Scientific Reports article

Errors in Figure 8

Updated Materials and methods (flow cytometry)

Corrected Figure 8 legend and quantifications

[www. nature.com/scientificreports](www. nature.com/scientificreports) Correction: Ptbp1 knockdown induces conversion of rat spinal cord astrocytes into neuron like cells Xianghui Hu, Shiyuan Han, Zhimin Li, Yongning Li & JunGao Correction to: Scientific Reports https://doi.org/10.1038/s41598-025-19720-1, published online 14 October 2025 The original version of this Article contained an error in Figure 8, where the upper four panels of 8E were inadvertently repeated in the lower four panels of 8B. The original Figure 8 and accompanying legend appears below. In addition, in the Materials and methods section, under the subheading ‘Non-reactive, reactive, and reversereactive astrocyte trans-differentiation in vitro’, the experimental method description for the flow cytometry results of Figure 2A was omitted. As a result,“The medium was half-changed every 3 days.” now reads: “The medium was half-changed every 3 days. Following a 48-hour transduction with GFP-lentivirus, transduction efficiency was quantified by flow cytometry, gating GFP-positive cells based on the blank control before analyzing the experimental groups.” [www. nature.com/scientificreports/](www. nature.com/scientificreports/) [www. nature.com/scientificreports/](www. nature.com/scientificreports/) ◂Fig. 8. The Shh signaling pathway agonist SAG facilitated the MAP2 expression of immature converted neuron-like cells. (A) Representative images of immunofluorescence staining of astrocytes transduced by shPTB-1 and shPTB-2 after four weeks, showing GFP (green) and the mature neuron marker MAP2 (red, Alexa Fluor 647) . Scale bar: 100 μm. (B) Representative images of immunofluorescence staining of astrocytes transfected by siRNA-PTB-1 and siRNA-PTB-2 after three weeks, showing Ptbp1 (green, Alexa Fluor 488) and the mature neuron marker MAP2 (red, Alexa Fluor 647) . Scale bar: 100 μm. (C) Representative images of immunofluorescence staining of astrocytes transfected by siRNA-PTB-1 or siRNA-Ctrl after two weeks, showing Ptbp1 (green, Alexa Fluor 488), the neuronal marker Tuj1 (red, Alexa Fluor 647), and the motor neuron-specific marker ChAT (indigo, Alexa Fluor 555). Some Ptbp1-Tuj1+ cells stained positive for ChAT. Scale bar: 50 μm. (D) Quantification of ChAT+ cells within Tuj1+ cells at two weeks in the siRNA-PTB-1 group and siRNA-Ctrl group. (E) Representative images of immunofluorescence staining of astrocytes transfected by siRNA-PTB-1 after two weeks, with or without SAG treatment, showing Ptbp1 (green, Alexa Fluor 488) and the mature neuron marker MAP2 (red, Alexa Fluor 647) . Scale bar: 100 μm. Several Ptbp1-cells were positive for MAP2 in the siRNA-PTB-1+ SAG group, whereas almost no Ptbp1-MAP2+ cells were detected in the siRNA-PTB-1 group. (F) Quantification of MAP2+ cells within Ptbp1-cells at two weeks in the siRNAPTB-1+ SAG group, compared to the siRNA-PTB-1 group (two-tailed Student’s t-test) . (G) Representative images of immunofluorescence staining of astrocytes transfected by siRNA-PTB-1 after two weeks, with or without SAG treatment, showing Ptbp1 (green, Alexa Fluor 488), the neuronal marker Tuj1 (red, Alexa Fluor 647), and the mature neuron marker MAP2 (indigo, Alexa Fluor 555) . Scale bar: 100 μm. Many Ptbp1-cells coexpressed Tuj1 and MAP2 in the siRNA-PTB-1+ SAG group, whereas they were positive for Tuj1 but negative for MAP2 in the siRNA-PTB-1 group. The experiments were performed three times. All data are presented as means ± SD. ***P < 0.001. n.d.: not detected; Ptbp1: polypyrimidine tract-binding protein; Tuj1: tubulin beta 3; MAP2: microtubule-associated protein 2; ChAT: Choline Acetyltransferase. The original Article has been corrected. Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original au

FAQs

What was the main issue corrected in the original Figure 8?

The original Figure 8 contained an error where the upper four panels of 8E were inadvertently repeated in the lower four panels of 8B.

What additional method information was missing for Figure 2A flow cytometry?

The Materials and methods description for the flow cytometry results of Figure 2A was omitted; it now includes the half-medium change schedule and the 48-hour GFP-lentivirus transduction with flow-cytometry quantification and GFP-positive gating based on blank control before analyzing experimental groups.

How does SAG treatment relate to MAP2 expression in the corrected figure?

The corrected Figure 8 indicates that the Shh signaling pathway agonist SAG facilitates MAP2 expression in immature converted neuron-like cells, with corresponding immunofluorescence images and quantification comparing SAG-treated versus untreated groups.

Sep 30, 2026

Upload by Oliver