Isatis tinctoria Köklerinde Maya Özütü, Kitosan ve Pektinin İndigotin ile İndirubin Birikimi Üzerine Etkileri

Aug 26, 2026

Root-derived secondary metabolites are valuable for medicine, pharmacy, cosmetics, agriculture, food, and textiles, yet collecting plants from nature threatens endemic and over-harvested species. In vitro production can provide standardized quantity and quality throughout the year, especially when elicitors stimulate biosynthesis pathways. This study evaluated yeast extract, chitosan, and pectin as cost-effective biotic elicitors by applying multiple concentrations to 21-day-old roots for 7 days. Root growth parameters and indigotin/indirubin contents were assessed after harvest to identify the most suitable elicitor doses.

LanguageEnglish

CategoryHealthcare

FormatPDF

Pages23

Reading Time58 min

AccessFree to download

Root growth and metabolite accumulation

Elicitor rationale and in vitro production

Experimental design and treatments

Evaluation after harvest

Isparta Uygulamalı Bilimler Üniversitesi Ziraat Fakültesi: Isparta Uygulamali Bilimler Universitesi Ziraat Fakultesi [https://orcid.org/0000-0002-8320-4142](https://orcid.org/0000-0002-8320-4142) Isparta University of Applied Sciences Faculty of Agriculture: Isparta Uygulamali Bilimler Universitesi Ziraat Fakultesi Isatis tinctoria, Indigotin, Indirubin, Chitosan, Yeast extract, Pectin December 23rd, 2024 [https://doi.org/10.21203/rs.3.rs-5608568/v1](https://doi.org/10.21203/rs.3.rs-5608568/v1) 􀁱 􀅏 This work is licensed under a Creative Commons Attribution 4.0 International License. Read Full License This study was carried out to determine the effects of yeast extract, chitosan, and pectin as simple and cost-effective biotic elicitors on root growth and the accumulation of indigotin and indirubin in the root of Isatis tinctoia. For this purpose, different concentrations of yeast extract (1, 2, 3, and 4 g L− 1), chitosan (100, 150, 200, and 250 mg L − 1), and pectin (0 . 5, 1, 2, and 3%) were applied to 21 days old roots for 7 days. After harvest, roots were evaluated regarding fresh root weight, root growth index, dry root weight, and contents of indigotin and indirubin. As a result of the study, it was determined that yeast extract showed positive effects on root growth while chitosan inhibited. On the other hand, pectin had no positive or negative impact on root growth parameters. Yeast extract and chitosan increased indigotin accumulation in roots compared to control roots while indigotin amounts decreased with the pectin. Within yeast extract applications, the highest indirubin contents were obtained from the roots applied with 3 and 4 g L − 1 of yeast extract. All chitosan applications enhanced the indirubin accumulation compared to control. The effect of pectin on indirubin accumulation was changed depending on its concentrations. Pectin at 0.5 and 1% increased indirubin contents compared to control. In conclusion, 1 g L − 1 of yeast extract for root growth and 100 mg L − 1 of chitosan for indigotin and indirubin contents were selected as the most appropriate applications supplying the highest values. Yeast extract showed positive effects on root growth while chitosan inhibited Pectin had no positive or negative impact on root growth parameters Among the applications, 1 g/L of yeast extract for root growth was the most appropriate application Yeast extract, chitosan, and pectin enhanced the indigotin and indirubin accumulation depending on their concentrations The highest indigotin and indirubin contents were obtained from the roots applied with 100 mg/L ofchitosan Plant secondary metabolites, essential components of the plant's defense system against various stress factors, have gained signi«cant popularity in recent years due to their use in various «elds such as medicine, pharmacy, cosmetics, perfumery, agriculture, food, and textiles (Fazili et al. 2023) . The most common method used to obtain these valuable metabolites synthesized in plants is the collection of plants from nature (Selwal, et al. 2023) . However, meeting the demand by collecting from nature poses a great danger to the survival of endemic and over-collected plant species, especially those with rootderived metabolites (Wawrosch and Zotchev 2021) . In addition, the emergence of quality and quantity differences due to climatic and geographical conditions in the production of secondary metabolites, the destruction of plants due to disease, pests, or unfavourable environmental conditions, the necessity of harvesting at certain times of the year, and unsuitable geographical conditions constitute other negative aspects of this metabolite production technique performed by traditional methods (Fazili et al. 2022) . Asa result of the search for alternative production techniques that eliminate these drawbacks and ensure standardized quantity and quality production throughout the year, it has been found that in vitro techniques present si

FAQs

Yeast extract, kitosan ve pektin neden çalışmada kullanıldı?

Bu çalışmada, in vitro koşullarda bitkilerde stres tepkisini ve sekonder metabolit üretim yollarını uyarabilen biyotik elicitor olarak maya özütü, kitosan ve pektin değerlendirilmiştir.

Uygulamalar köklerde hangi büyüme göstergeleri ve bileşikler üzerinden değerlendirildi?

Hasat sonrası taze ve kuru kök ağırlığı, kök büyüme indeksi ile indigotin ve indirubin içerikleri incelenmiştir.

Çalışmanın sonuçlarına göre hangi elicitorlar daha etkili oldu?

Maya özütü kök büyümesini artırırken kitosan büyümeyi inhibe etti. İndigotin ve indirubin birikimi, uygulanan elicitorun türü ve konsantrasyonuna bağlı olarak değişti.

Aug 26, 2026

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