Caspase-3 and CD68 Immunoreactivity in Lymphoid Tissues and Hematology of Rats Exposed to Cisplatin and L-carnitine

Aug 26, 2026

Study investigated caspase-3 and CD68 activity in lymphoid tissues of rats subjected to cisplatin (CDDP)-induced toxicity and evaluated L-carnitine (L-Car) protective effects. Rats received saline control, CDDP alone (7 mg/kg i.p.), L-Car alone (500 mg/kg i.p.), or L-Car for 10 days followed by CDDP. Compared with control, CDDP reduced total WBC with altered leukocyte distributions, while L-Car plus CDDP restored total WBC and increased neutrophil/lymphocyte counts relative to CDDP. Cisplatin increased apoptosis and reduced macrophage activity in lymphoid organs, especially lymph node.

LanguageEnglish

CategoryHealthcare

FormatPDF

Pages8

Reading Time20 min

AccessFree to download

Introduction

Cisplatin effects and toxicity

Apoptosis and caspase-3

CD68 as macrophage marker

L-carnitine role and prior protective studies

Caspase-3 and CD68 Immunoreactivity in Lymphoid Tissues and Haematology of Rats Exposed to Cisplatin and L-carnitine Ertan ORUC *􀀇 Adem KARA ** Ismail CAN ** Ali KARADENIZ *** Nejdet SIMSEK ** * Atatürk University, Faculty of Veterinary Medicine, Department of Pathology, TR-25240 Erzurum-TURKEY ** Atatürk University, Faculty of Veterinary Medicine, Department of Histology and Embryology, TR-25240 ErzurumTURKEY *** Atatürk University, Faculty of Veterinary Medicine, Department of Physiology, TR-25240 Erzurum-TURKEY Makale Kodu (Article Code): KVFD-2012-6796 Summary This study was performed to determine caspase-3 and CD68 activities in lymphoid tissues of rats exposed to Cisplatin (CDDP) induced toxicity and L-carnitine (L-Car) protection. Group control was received single-dose intraperitoneal (i.p.) 1 ml isotonic saline, Group L-Car received single-dose injections of L-carnitine 500 mg/kg bw. i.p., Group CDDP received a single-dose injection of CDDP 7 mg/kg bw i.p. all at once, Group L-Car + CDDP received single doses of L-carnitine 500 mg/kg bw i.p. for 10 consecutive days and following a single-dose i.p. injection of CDDP 7 mg/kg bw i.p. When compared to the control group, the numbers of total WBC were decreased while a relative increase in lymphocyte percentages was observed in the CDDP-treated group. On the other hand, relative decreases in neutrophil and monocyte percentages were observed in the CDDP-treated group compared to control group. Conversely, L-Car treatment with CDDP injections caused significantly increased the number of total WBC. Caspase-3 immunopositivity was found in varying degree in lymphoid tissues. L-carnitine supplementation to diet decreased apoptotic effect of cisplatin. CD68 positive cells were found similar in control and L-Car groups. While CD68 positivity was found in low degree in CDDP group, it was moderate in L-Car + CDDP group. It is concluded that cisplatin increases apoptosis and decrease macrophage activity in lymphoid tissues. This both effects were found especially in lymph node in high degree and thymus in low degree. Keywords: Carnitine, Caspase-3, Cisplatin, CD68, Lymphoid organ Sisplatin ve L-karnitin’e Maruz Kalmış Ratların Lenfoid Dokularında Caspase-3 ve CD68 Immunoreaktivitesi ve Hematolojisi Özet Bu çalışmada sisplatin (CDDP) toksikasyonuna maruz kalmış ve dietlerine L-carnitine ilave edilmiş ratların lenfoid dokularında Caspase-3 ve CD68 aktivitesinin belirlenmesi ve elde edilen sonuçların hematolojik bulgularla karşılaştırılması amaçlanmıştır. Bu amaçla kontrol grubunu oluşturan ratlara tek doz peritoniçi (i.p.) izotonik salin, L-car grubuna tek doz 500 mg/kg (i.p.) L-carnitine, CDDP grubuna tek doz 7 mg/kg (i.p.) CDDP, ve son olarak CDDP + L-car grubuna 10 gün boyunca tek doz 500 mg/kg (i.p.) L-carnitine ve akabindetek doz 7 mg/kg (i.p.) CDDP uygulandı. Deneme sonrası elde edilen bulgular doğrultusunda, kontrol grubu ile kıyaslandığında CDDP grubunda lenfosit oranında artış varken toplam WBC oranı azalmıştı . Diğer tarafdan nötrofil lökosit ve monosit oranlarında da CDDP grubunda kontrol grubuna oranla azalma görüldü . Bu bulguların aksine L-car+CDDP grubundatoplam WBC, nötrofil lökosit ve lenfosit miktarında CDDP grubuna oranla artış tespit edildi. Caspase-3 immunopozitifliği lenfoid dokularda değişen derecelerde tespit edilirken, diyete L-carnitine ilavesi ile sisplatinin apoptotik etkisinde azalma görüldü . CD68 pozitiflik oranı kontrol, L-car ve L-car + CDDP gruplarında birbirine yakın ve orta şiddette bulunurken CDDP grubunda CD68 pozitiflik oranı daha düşük düzeyde tespit edildi. Elde edilen bulgular ışığında, sisplatinin lenfoid dokularda apoptozisi arttırdığı, makrofaj aktivitesini ise azalttığı, her iki etkinin özellikle lenf düğümünde daha belirgin, timusda ise daha az etkide olduğusonucuna varıldı . Anahtarsözcükler: Caspase-3, Cd68, Lenfoid organ, L-karnitin, Sisplatin 􀀇 İletişim (Correspondence) 􀀊 +90 442 2315557 􀀍 [eoruc@atauni.

FAQs

What was the purpose of measuring caspase-3 and CD68 in this study?

To determine whether cisplatin-induced toxicity increases apoptosis (via caspase-3) and alters macrophage-related activity (via CD68) in rat lymphoid tissues, and whether L-carnitine can mitigate these effects.

How were the experimental groups treated?

Control received single-dose isotonic saline; L-Car received single-dose L-carnitine (500 mg/kg i.p.); CDDP received single-dose cisplatin (7 mg/kg i.p.); and L-Car + CDDP received L-carnitine for 10 consecutive days followed by a single cisplatin injection.

What hematological and tissue changes did cisplatin and L-carnitine produce?

Cisplatin decreased total WBC and changed leukocyte proportions, and showed variable caspase-3 positivity with reduced CD68 positivity in the CDDP group. L-carnitine supplementation with cisplatin significantly increased total WBC and attenuated cisplatin’s apoptotic effect, with CD68 positivity being moderate in the L-Car + CDDP group.

Aug 26, 2026

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