The extract of zedoary-turmeric protects rats' kidneys from damage caused by cisplatin

Sep 30, 2026

Uploaded by Rowan

Objective: examine how the combination of Curcuma zedoaria (zedoary) and Curcuma longa (turmeric) influences kidney outcomes in rats with cisplatin-induced acute kidney injury (AKI), focusing on reductions in Caspase-3, KIM-1, and TNF-α within renal tissue. Materials and Methods: five rat groups (normal control, cisplatin control, and Ext100/200/400 extract treatments) assessed on day 10 for body and kidney weights, histology, and KIM-1, TNF-α, and Caspase-3 gene expression via qRT-PCR with SPSS 29.0 statistics. Results and Conclusion: Ext200 increased body weight and reduced histological damage, with markedly lower gene expression, indicating 200 mg/kg combined extract may prevent or lessen cisplatin kidney injury.

LanguageEnglish

CategoryHealthcare

FormatPDF

Pages9

Reading Time23 min

AccessFree to download

Abstract

Introduction

Materials and Methods

Results

Conclusion

ORIGINAL ARTICLE The extract of zedoary-turmeric protects rats’ kidneys from damage caused by cisplatin Putri Reno Intan1,2, Lisa Andriani Lienggonegoro2, Frans Dany2, Ariyani Noviantari2, Uly Alfi Nikmah2, Sukmayati Alegantina3, Ratih Rinendyaputri2, Ani Isnawati3, Sunarno Sunarno2, Indah Fajarwati2, Sela Septima Mariya2, Agus Setiyono4, Lina Noviyanti Sutardi5, Ekowati Handharyani4 1Animal Biomedical Study Program, IPB Postgraduate School, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia 2Center for Biomedical Research, Research Organization for Health, National Research and Innovation Agency (BRIN), Cibinong Science Center, Bogor, Indonesia 3 Research Center for Pharmaceutical Ingredients and Traditional Medicine, Research Organization for Health, National Research and Innovation Agency (BRIN), Cibinong Science Center, Bogor, Indonesia 4School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia 5 Division of Pharmacy, School of Veterinary Medicine and Biomedical Sciences, IPB University, Bogor, Indonesia ABSTRACT Objective: The goal of this study was to examine how the combination of Curcuma zedoaria (zedoary) and Curcuma longa (turmeric) affected things on the kidneys of rats with acute kidney injury (AKI) from cisplatin by assessing the reduction of levels of cysteine-aspartic acid protease 3 (Caspase-3), kidney injury molecule-1 (KIM-1), and tumor necrosis factor-alpha (TNF-α) in renal tissue. Materials and Methods: There were five groups of rats: a normal control group, a cisplatin control group (CP), and three extract treatment groups (Ext100, Ext200, and Ext400) . The CP group got cisplatin on day seven to cause AKI, while the extract group got cisplatin on day seven and the combined extract on days one through nine. On the 10th day, we looked at body weight, kidney weight, histology, and gene expression of KIM-1, TNF-α, and Caspase-3 by quantitative real-time polymerase chain reaction. We used SPSS (version 29.0) to do the statistical analyses. We present the data as mean ± SD or SEM and use analysis of variance and Tukey’s post-hoc test to analyze them. Results: Body weight decreased in the CP group, while it initially decreased and then increased in the extract groups, with Ext200 showing the greatest increase. Histologically, the CP group exhibited severe kidney damage, whereas the Ext200 group showed reduced damage. Gene expression of Caspase-3, KIM-1, and TNF-α was much lower in the Ext100 and Ext200 groups than in the CP group. Conclusion: Cisplatin-induced AKI caused less damage to the kidneys and less production of KIM- 1, TNF-α, and Caspase-3, suggesting that a 200 mg/kg combined extract of Zedoary and Turmeric could be used to prevent or lessen kidney damage. These results show that the extract could preserve the kidneys, which could lead to the creation of other treatments for those who are receiving cisplatin. ARTICLE HISTORY Received January 13, 2025 Revised March 06, 2025 Accepted March 16, 2025 Published September 10, 2025 KEYWORDS Acute kidney injury; cisplatin; Curcuma longa; inflammation; rat; zedoaria © The authors. This is an Open Access article distributed under the terms of the Creative Commons Attribution 4.0 License ([http://creativecommons.org/](http://creativecommons.org/)[ ](http://creativecommons.org/)[licenses/by/4.0](licenses/by/4.0)) Introduction Acute kidney injury (AKI) is when the kidneys do not work properly, which can be seen in changes in urine production and high levels of urea and creatinine in the blood [1] . It is linked to the higher death rates, longer hospital stays, and heart problems [2]. AKI is a big problem for people’s health around the world. It affects about 13 million people and kills 1.7 million every year [3,4] . Acute kidney toxicity can arise from various causes, with nephrotoxic injury being a leading factor in acute tubular necrosis [5] . Cisplatin is

FAQs

What was the main objective of this study?

To evaluate how combined Curcuma zedoaria (zedoary) and Curcuma longa (turmeric) affects kidney outcomes in rats with cisplatin-induced AKI by measuring Caspase-3, KIM-1, and TNF-α in renal tissue.

How were the rats treated in the experimental groups?

Five groups were used: normal control, cisplatin control (CP), and three extract groups (Ext100, Ext200, Ext400). CP received cisplatin on day 7, while the extract groups received cisplatin on day 7 and the combined extract from days 1 to 9.

Which extract dose showed the strongest protective effect and what evidence supported it?

Ext200 showed the strongest effect, with the greatest increase in body weight and reduced histological kidney damage. It also produced much lower gene expression of Caspase-3, KIM-1, and TNF-α compared with the cisplatin control group.

Sep 30, 2026

Uploaded by Rowan