tube-type - Blood collection tube types order

Oct 13, 2026

Instructions for collecting blood for evacuated tube systems specify the required order of draw and the tube types associated with a wide range of laboratory tests. Guidance includes maintaining aseptic technique for blood culture bottles, collecting aerobic before anaerobic, and strictly filling tubes to the indicated blood-to-anticoagulant ratio. It also provides handling requirements such as inverting selected tubes multiple times and using ice for temperature-sensitive specimens (e.g., ammonia), covering coagulation, serology, chemistry, molecular, and trace metal assays.

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Collect the blood into the containers in the order listed below for evacuated systems. For needle and syringe order of draw refer to Prompt Document: Blood Collection order of draw for tubes and bottles | Tube Type | Tests | Instructions | | --- | --- | --- | | Blood Culture Bottles | Blood Culture | Ensure aseptic technique. Collect Aerobic, then
Anaerobic | | | APTT, Coagulation profile, D-dimer, Factor assays, heparin assay, INR, Lupus anticoagulant, | Fill to mark as the ratio of blood to anticoagulant is critical. | | | Alpha fetoprotein, Amylase, ANA, ANCA, Antenatal Serology, Beta HCG (BHCG), Calcium, carbamazepine (Tegretol), Carcino embryoic antigen (CEA), Cardiac enzymes (CE), Cholesterol (Lipids), CKMB, Clonazepam (Rivotril), CMV, Coeliac Serology, C-Reactive Protein(CRP), Creatinine, Digoxin, dsDNA, EBV, Electrolytes, ENA, estradiol (ADULT), Ethanol, Ferritin, Follicle stimulating hormone (FSH), Free thyroxine(FT4), gentamicin, Gliadin antibodies (DGP), HDL cholesterol, Hepatitis, HIV, Infectious Disease Serology (IDS), Iron (Fe) studies, LFT's, Lipase, lithium, Luteinizing hormone (LH), Measles, Mumps, Mycoplasma pneumoniae, Needlestickserology, paracetamol, Phenytoin (Dilantin), Phosphate (PO4), Progesterone(Prog), Prolactin (PRL), Prostatic specific antigen (PSA), Protein studies/electrophoresis (SPEP), Rubella, Streptococcus(ADB,ASOT), Syphilis, theophylline, Thyroid stimulating hormone (TSH), Tissue transglutaminase antibodies (TTG), Toxoplasma, Triglyceride, troponin I, Urea, Uric Acid (UA), valproate (Epilim), vancomycin, Varicella | Invert 6-8 times | | | clozapine, Sensitive estradiol (Children) , Testosterone, dihydrotestosterone, Androstenedione, 17 hydroxy progesterone | Invert 6-8 times | | | ESR | Fill to line
Invert 6-8 times | | | Chromosome studies, Karyotype, FISH | Invert 6-8 times | | | Ammonia (on ice), Amylase, Calcium, carbamezapine, Cardiac enzymes, Creatinine, CRP, Digoxin, Electrolytes, Ethanol, gentamicin, Iron, Lipase, LFTs, Magnesium, paracetamol, Phenytoin, Salicylate, Theophylline, Troponin I, Vancomycin, Urea | Invert 6-8 times | | | Copper, Cobalt, Chromium, Manganese, Zinc (Zn) | Invert 6-8 times | | | Blood group / Hold / Antibody Screen / Crossmatch, | Invert 6-8 times | | | FBE, WCC, Differential, Haemoglobin, Platelets, Haemoglobin electrophoresis, Coombs (DCT), Fragile X, Malaria parasites, Neisseria meningitidis PCR, Bartonella henselae PCR, HIV Proviral DNA, Reticulocytes, Thalassaemia DNA, Haemophilia, Haemochromatosis, Microarray (Molecular Karyotype), Molecular genetic testing | Invert 6-8 times | | | CMV PCR/Viral Load, HCV Genotype, Hepatitis B PCR, Hepatitis C PCR, Herpes Multiplex PCR, HIV PCR/Genotype/Resistance testing, EBV PCR/Viral Load, BK Virus | Invert 6-8 times | | | Glucose (random or fasting), Lactate (lactic acid) | Invert 6-8 times | | | HLA | Invert 6-8 times | | TEST | TUBE | | TEST | TUBE | | | --- | --- | --- | --- | --- | --- | | Alpha fetoprotein | | Serum SST | Haemophilia genetic testing | | EDTA-3mL | | Ammonia (on ice) | | Li Hep-Gel | HCV Genotype | | PPT | | Amylase | | Serum SST | HDL cholesterol | | Serum SST | | ANA | | Serum SST | heparin assay | | Sodium Citrate | | ANCA | | Serum SST | Hepatitis B/Hepatitis C PCR | | PPT | | Antenatal Serology | | Serum SST | Hepatitis serology | | Serum SST | | APTT | | Sodium Citrate | Herpes Multiplex PCR | | PPT | | Beta HCG (BHCG) | | Serum SST | HIV PCR | | PPT | | Blood group | | EDTA-10mL | HIV serology | | Serum SST | | Blood group Antibody screen | | EDTA-10mL | Homocysteine (on ice) | | EDTA-3mL | | Calcium | | Serum SST | Infectious Disease Serology (IDS) | | Serum SST | | carbamazepine (Tegretol) | | Serum SST | INR | | Sodium Citrate | | Carcino embryonic antigen (CEA) | | Serum SST | Iron (Fe) or Iron studies | | Serum SST | | Cardiac enzymes (CE's) | | Serum SST | Lactate (lactic acid) | | Fluoride-EDTA | | Cholesterol (Lipids) | | Serum SST | LFT'

FAQs

What is the general principle for collecting blood into evacuated system containers?

Collect blood into the listed containers in the order specified for evacuated systems, following the provided order of draw guidance.

How should blood culture bottles be handled during collection?

Use aseptic technique and collect aerobic first, then anaerobic.

Why is filling to the correct blood-to-anticoagulant ratio important?

The instructions state the ratio is critical, and tubes should be filled to the required mark to ensure valid results.

Oct 13, 2026

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