BSL-2 Biosafety Manual - Lab-Specific Template - Training, Signage, Cabinet, and Disposal

Aug 31, 2026

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BSL-2 biosafety manual template for a lab setting, covering core requirements for working with Risk Group 2 biological agents. Includes sections for research description and protocol-specific risk assessment, required personnel training (EHS-recommended modules and competency demonstration), and detailed laboratory signage practices with permanent and alternative postings. Provides guidance for biosafety cabinet use, safe handling and disposal expectations, spill response, emergency procedures, post-exposure actions, animal work, cryogenic liquid safety, and appendices for contacts, references, and training records.

LanguageEnglish

CategoryForms

FormatDOCX

Pages24

Reading Time8 min

AccessFree to download

I. INTRODUCTION AND DESCRIPTION OF RESEARCH

II. TRAINING

III. LABORATORY SIGNAGE

IV. USE OF BIOLOGICAL SAFETY CABINET

V. USE AND DISPOSAL OF SHARPS

VI. SPILL RESPONSE PROCEDURES

VII. WASTE DISPOSAL PROCEDURES

VIII. EMERGENCY PROCEDURES

IX. Post-Exposure Fact Sheet

X. WORKING WITH ANIMALS

XI. SAFE HANDLING OF CRYOGENIC LIQUIDS

APPENDIX 1: EMERGENCY CONTACTS

APPENDIX 2: REFERENCES

APPENDIX 3: AGENT SUMMARY STATEMENT (OPTIONAL)

Appendix 4. BMBL BSL-2 Laboratory Criteria

Appendix 5. Lab Staff Training Records

Appendix 6. Principal Investigator’s Registered Research

ENTITY/UNIVERSITY BSL-2 BIOSAFETY MANUAL Dr. ___’s Lab ___ Building Rooms ___ Contents TOC \o "1-3" \h \z \u HYPERLINK \l "_Toc330369751" I. INTRODUCTION AND DESCRIPTION OF RESEARCH PAGEREF _Toc330369751 \h 3 HYPERLINK \l "_Toc330369752" II. TRAINING PAGEREF _Toc330369752 \h 4 HYPERLINK \l "_Toc330369753" III. LABORATORY SIGNAGE PAGEREF _Toc330369753 \h 5 HYPERLINK \l "_Toc330369754" IV. USE OF BIOLOGICAL SAFETY CABINET PAGEREF _Toc330369754 \h 6 HYPERLINK \l "_Toc330369755" V. USE AND DISPOSAL OF SHARPS PAGEREF _Toc330369755 \h 8 HYPERLINK \l "_Toc330369756" VI. SPILL RESPONSE PROCEDURES PAGEREF _Toc330369756 \h 10 HYPERLINK \l "_Toc330369757" VII. WASTE DISPOSAL PROCEDURES PAGEREF _Toc330369757 \h 11 HYPERLINK \l "_Toc330369758" VIII. EMERGENCY PROCEDURES PAGEREF _Toc330369758 \h 12 HYPERLINK \l "_Toc330369759" IX. Post-Exposure Fact Sheet PAGEREF _Toc330369759 \h 13 HYPERLINK \l "_Toc330369760" X. WORKING WITH ANIMALS PAGEREF _Toc330369760 \h 14 HYPERLINK \l "_Toc330369761" XI. SAFE HANDLING OF CRYOGENIC LIQUIDS PAGEREF _Toc330369761 \h 15 HYPERLINK \l "_Toc330369762" APPENDIX 1: EMERGENCY CONTACTS PAGEREF _Toc330369762 \h 16 HYPERLINK \l "_Toc330369763" APPENDIX 2: REFERENCES PAGEREF _Toc330369763 \h 17 HYPERLINK \l "_Toc330369764" APPENDIX 3: AGENT SUMMARY STATEMENT (OPTIONAL) PAGEREF _Toc330369764 \h 18 HYPERLINK \l "_Toc330369765" Appendix 4. BMBL BSL-2 Laboratory Criteria PAGEREF _Toc330369765 \h 19 HYPERLINK \l "_Toc330369766" Appendix 5. Lab Staff Training Records PAGEREF _Toc330369766 \h 24 HYPERLINK \l "_Toc330369767" Appendix 6. Principal Investigator’s Registered Research PAGEREF _Toc330369767 \h 25 I. INTRODUCTION AND DESCRIPTION OF RESEARCH Insert brief description of research. Biological agents/organisms used: (include bacterial, viral, fungal, recombinant, human/non-human primate unfixed tissues or cells) List organisms/biohazards here Risk Assessment: Please provide a brief protocol-specific risk assessment. Include consideration of parent and recombinant agent pathogenicity, virulence, infectious dose, route of transmission, host range, and stability, as well as the likelihood of exposure and consequences of exposure. How will identified risks be controlled (e.g. PPE, work practices, etc.)? Note that “replication incompetent” does not mean “non-infectious.” Research involving recombinant/synthetic nucleic acids, infectious agents, creation of transgenic animals, unfixed non-human primate materials, or human blood, cells, or unfixed tissues must submit a research registration form through EHS at least every four years. This work may require prior review and approval by the Institutional Biosafety Committee, according to the NIH Guidelines for Research Involving Recombinant DNA Molecules. Since the university receives funding from NIH, all research at UM must comply with the NIH Guidelines. This plan was designed as a supplement to the University Biosafety Manual to meet the requirements of Biosafety in Microbiological and Biomedical Laboratories, 5th ed. II. TRAINING All personnel who work in the laboratory must receive adequate instruction from their supervisor prior to beginning work. Some training is required annually. Each lab will require different trainings. For biosafety, EHS recommends: BSL2 training Bloodborne Pathogens training NIH Guidelines for Research Involving Recombinant/Synthetic Nucleic Acid Molecules Working Safely With Laboratory Animals Spill Prevention, Control, and Countermeasure Safe Handling and Use of Cryogenic Liquids Hazardous Waste Generator Shipping Infectious Materials: Training is required to commercially transport infectious materials. Contact EHS to schedule a training class. Training on lab-specific techniques and demonstration of competency should also be required before work. Some work may require an occupational heal

FAQs

What must be included in the research introduction and protocol-specific risk assessment?

The template requires a brief description of the research, a list of biological agents/organisms used, and a protocol-specific risk assessment addressing factors such as pathogenicity, virulence, infectious dose, route of transmission, host range, stability, exposure likelihood, and exposure consequences. It also asks how identified risks will be controlled through work practices and PPE.

What training is required for laboratory personnel working under BSL-2?

Personnel must receive adequate instruction from their supervisor before starting work, with some training required annually. EHS recommends specific BSL-2 training topics including Bloodborne Pathogens, NIH recombinant/synthetic nucleic acid guidelines, safe animal work, spill prevention and countermeasures, cryogenic liquid safety, hazardous waste generator training, and shipping infectious materials training when applicable.

How should laboratory signage be handled for BSL-2 work?

Permanent yellow warning signs with the biohazard symbol and BSL-2 designation must be posted at entrances where Risk Group 2 agents are used or stored long-term. Biosafety level 2 labs should also use permanently affixed decals for the biohazard symbol, “BSL-2,” and exit requirements. Alternative supplemental signage is used when the agent will be removed and the room decontaminated frequently, and the biohazard/BSL-2 markings are removed from the permanent sign when that system is active.

Aug 31, 2026

Upload by Asher