TRIM69 - a marker of metastasis and potential sensitizer to 5-Fluorouracil and PD-1 blockers in colon adenocarcinoma

Sep 24, 2026

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Study of colorectal cancer (CRC) focuses on TRIM-family proteins, yet the role of TRIM69 in colon adenocarcinoma (COAD) remained unclear. This work correlates TRIM69 expression with clinical features and survival using TCGA mRNA sequencing and predicts immune and chemosensitivity links via multiple bioinformatics platforms. Experimental validation in patient tissues uses PCR and immunohistochemistry. Lower TRIM69 in COAD relates to pathological stage and metastasis, with pathway enrichment including NOD-like signaling, suggesting improved sensitivity to 5-fluorouracil and PD-1 blockade.

LanguageEnglish

CategoryHealthcare

FormatPDF

Pages11

Reading Time28 min

AccessFree to download

Abstract

Background

Methods

Results

Conclusions

Background

CRC risk factors and treatment context

TRIM protein family and TRIM69 rationale

Chi et al. BMCGastroenterology (2023) 23:292 [https://doi.org/10.1186/s12876-023-02927-9](https://doi.org/10.1186/s12876-023-02927-9) BMC Gastroenterology RESEARCH Open Access TRIM69: a marker of metastasis and potential sensitizer to 5-Fluorouracil and PD-1 blockersin colon adenocarcinoma Xiao-Jv Chi 1†, Yi-Bei Song 1†, Deng-He Liu 1, Li-Qiang Wei 1, An-Ran Zhao 1, Xin An2, Zi-Zhen Feng2, Xiao-Hua Lan2, Yu-Meng Lv2, Hong-jun Li2, Dong Lan3* and Hui-Min He2* Abstract Background Several proteins in the tripartite-motif (TRIM) family are associated with the development of colorectal cancer (CRC), but research on the role of TRIM69 was lacking. The present study examined the correlation between TRIM69 expression and colon adenocarcinoma (COAD) . Methods mRNA sequencing data for COAD patients was extracted from The Cancer Genome Atlas to analyze correlations between TRIM69 expression and patients’ clinical features as well as survival. Potential associations with immune cells and chemosensitivity also were predicted using various algorithms in the TIMER, Limma, clusterProfiler, GeneMANIA, and Gene Set Cancer Analysis platforms. Subsequently, polymerase chain reaction analysis and immunohistochemical staining were used to detect TRIM69 expression in COAD tissue samples from real-world patients. Results TRIM69 expression was lower in COAD tissues than in normal tissues and correlated with the pathologic stage and metastasis (M category) . Additionally, TRIM69 was found to be involved in several immune-related pathways, notably the NOD-like signaling pathway. These results suggest that high TRIM69 expression has the potential to enhance tumor sensitivity to 5-fluorouracil and programmed cell death protein 1 (PD-1) blockers. Conclusions From our findings that TRIM69 expression was significantly reduced in COAD compared with noncancer tissues and associated with pathologic stage and metastasis, we conclude that increasing TRIM69 expression and/or activity may help to improve therapeutic outcomes. Accordingly, TRIM69 represents a potentially valuable marker of metastasis and target for adjuvant therapy in COAD. Keywords TRIM69, NOD-like signaling pathway, 5-Fluorouracil, PD-1 blockers, COAD †Xiao-Jv Chi, Yi-Bei Song contributed equally to this work. *Correspondence: Dong Lan [landong@stu.gxmu.edu.cn](landong@stu.gxmu.edu.cn)[ ](landong@stu.gxmu.edu.cn)Hui-Min He[hehuimin@sr.gxmu.edu.cn](hehuimin@sr.gxmu.edu.cn) 1Department of Clinical Laboratory, the First Affiliated Hospital of Guangxi Medical University, Key Laboratory of Clinical Laboratory Medicine of Guangxi Department of Education, Guangxi Zhuang Autonomous Region, 6 Shuangyong Road, Nanning 530021, China 2Guangxi Medical University, Guangxi Zhuang Autonomous Region, 22 Shuangyong Road, Nanning 530021, China 3Department of Medical Oncology, the First Affiliated Hospital of Guangxi Medical University, Guangxi Zhuang Autonomous Region, 6 Shuangyong Road, Nanning 530021, China © The Author(s) 2023. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit [http://creativecommons.org/l](http://creativecommons.org/l)icenses/by/4.0/. The Creative Commons Public Domain Dedication waiver ([http://creativecommons.org/publ

FAQs

What is the main research question about TRIM69 in colon adenocarcinoma?

The study examines how TRIM69 expression relates to colon adenocarcinoma development and whether it connects to clinical features, survival, metastasis, and therapeutic sensitivity.

How were TRIM69 expression links to clinical outcomes analyzed?

mRNA sequencing data for COAD patients were extracted from The Cancer Genome Atlas to evaluate correlations between TRIM69 expression, clinical features, and survival.

What experimental approaches were used to validate TRIM69 in patient tissues?

Polymerase chain reaction analysis and immunohistochemical staining were used to detect TRIM69 expression in COAD tissue samples from real-world patients.

Sep 24, 2026

Uploaded by Aditya