Tracing the Anti-Inflammatory Mechanism/Triggers of D-Allulose: A Profile Study of Microbiome Composition and mRNA Expression in Diet-Induced Obese Mice

Jul 10, 2026

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The research article investigates how d-allulose modulates obesity-related inflammation in diet-induced obese mice by linking diet effects, gut microbiome composition, and tissue mRNA expression. Differentially expressed gene (DEG) analysis using liver and epididymal fat identifies genes whose expression recovers from high-fat-diet dysregulation. Correlation-based network analysis connects diet, microbial candidates, and 20 anti-obesogenic genes associated with Lactobacillus and Coprococcus, supporting reduced weight gain and inflammation.

LanguageEnglish

CategoryResearch & Report

FormatPDF

Pages10

Reading Time25 min

Introduction

Results and Discussion

Anti-Obesity Effects in Diet-Induced Obesity Mice

RESEARCH ARTICLE [www.mnf-journal.com](www.mnf-journal.com) Tracing the Anti-Inﬂammatory Mechanism/Triggers of d-Allulose: A Proﬁle Study of Microbiome Composition and mRNA Expression in Diet-Induced Obese Mice Youngji Han, Joon Yoon, and Myung-Sook Choi* Scope: The results of recent studies on d-allulose intervention in high-fat diet (HFD)-fed mice suggest that d-allulose has a substantial impact on obesity. In addition, several studies have uncovered bacterial candidates among the gut microbiota associated with obesity and inﬂammation in mice. To identify the d-allulose-attenuated genes related to the inﬂammation-associated bacterial candidates, two types of statistical analyses are performed. Methods and results: Using liver and epididymal fat tissues, genes with expression levels that recovered from HFD-induced dysregulation are identiﬁed through diﬀerentially expressed gene (DEG) analysis. Finally, correlation-based network analysis between the diet, microbes, and the candidates identiﬁed from DEG analysis reveal 20 genes that showed anti-obesogenic patterns and associations with Lactobacillus and Coprococcus, which are representative bacterial candidates associated withinﬂammation and obesity. Conclusion: The results of the present study suggest that d-allulose closely interacts with the candidate genes and microbes to alleviate weight gain and inﬂammation, partly via down regulation of Gm12250 expression in multiple tissues and increases the Lactobacillus and Coprococcus in gut microbiota composition. 2014.[1] As the prevalence of overweight and obesity in the United States grows, the prevalence of obesity-related diseases also increases. Obesity has rapidly grown to be one of the highest risk factors for the development of severe health issues such as diabetes, cancer, and cardiovascular diseases.[2–4] Previous studies have indicated that dietary intervention changes the composition of gut microbiota and that probiotics and polyphenol-rich fruit extracts reduce visceral adiposity and obesity in animals and humans.[5–7] Host-microbe studies have associated the composition of gut microbiota with the development of dietinduced obesity in animals through a relationship with the immune response, inﬂammation, and insulin resistance.[8] Based on the theory that gut microbiota may induce obesity, we focused on the well-known target genera of Lactobacillus and Coprococcus along with the overall microbiome proﬁles of our samples. d-allulose also known as d-psicose, a C-3 epimer of d-fructose, has 1. Introduction According to reports from the National Health and Nutrition Examination Survey, more than two in three adults in the United States (70.2%) were considered overweight or obese in 2013 and Dr. Y. Han, Prof. M.-S. Choi Department of Food Science and Nutrition Kyungpook National University 1370 San-Kyuk Dong Puk-Ku, 702-701 Daegu, Republic of Korea [E-mail: mschoi@knu.ac.kr](E-mail: mschoi@knu.ac.kr) Dr. Y. Han, Prof. M.-S. Choi Center for Food and Nutritional Genomics Research Kyungpook National University 1370 San-Kyuk Dong Puk-Ku 702-701 Daegu, Republic of Korea Dr. J. Yoon Harvard University T. H. Chan School of Public Health 655 Huntington Ave, Boston, MA 02115, USA The ORCID identiﬁcation number(s) for the author(s) of this article can be found under [https://doi.org/10.1002/mnfr.201900982](https://doi.org/10.1002/mnfr.201900982) DOI: 10.1002/mnfr.201900982 received substantial attention as a sugar substitute.[9] Several studies have provided evidence that d-allulose can improve diabetes and obesity by regulating glucose and lipid metabolism.[10–12] However, there has been no investigation on the regulation of high fat diet (HFD)-induced inﬂammation by dallulose supplementation with a multi-tissue experiment. Therefore, we performed an animal feeding study and evaluated both the biochemical alterations of mRNA expression and the microbiome composition by identifying diﬀer

FAQs

What is the main goal of this study on d-allulose?

To identify how d-allulose attenuates inflammation-associated triggers linked to obesity by integrating gene expression changes and gut microbiome profiles in high-fat-diet mice.

Which tissues and analyses are used to find relevant genes?

Liver and epididymal fat tissues are analyzed using differentially expressed gene (DEG) analysis to detect genes whose expression recovers from high-fat-diet dysregulation.

How are diet, microbes, and genes connected in the study?

Correlation-based network analysis links dietary factors, microbial candidates, and the DEG-identified genes, yielding 20 genes showing anti-obesogenic patterns associated with Lactobacillus and Coprococcus.

Jul 10, 2026

Upload by Jiven