TPP1 is associated with risk of advanced precursors and cervical cancer survival

Q: What is the main research question about TPP1 in cervical cancer?

The study investigates how TPP1 relates to hTERT and whether TPP1 expression influences cervical cancer development, progression, and patient survival.

Q: Which patient and lesion groups were analyzed in the study?

The analysis included 156 cervical cancer cases (2003–2008, followed to 2014), 102 CIN patients, and 16 participants with normal cervix identified in the same period.

Q: How does TPP1 affect hTERT and patient outcomes?

siRNA knockdown of TPP1 inhibited hTERT expression at mRNA and protein levels. High TPP1 expression and co-high TPP1/hTERT expression were independently associated with worse overall survival.

Sep 21, 2026

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Telomere-binding protein TPP1 interacts with hTERT, but its role in cervical cancer development and progression remains unclear. This study analyzed 156 eligible cervical cancer cases diagnosed during 2003–2008 with follow-up to 2014, alongside 102 CIN patients and 16 normal cervix participants. Expression correlations, siRNA-mediated TPP1 knockdown, mRNA/protein measurements, and Cox regression survival analyses were performed. High TPP1 expression correlated with CIN 3 and cervical cancer, inhibited hTERT after knockdown, and predicted worse overall survival.

LanguageEnglish

CategoryHealthcare

FormatPDF

Pages15

Reading Time38 min

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Abstract

Introduction

PLOS ONE OPEN ACCESS Citation: Wang Q-L, Gong C, Meng X-Y, Fu M, Yang H, Zhou F, et al. (2024) TPP1 is associated with risk of advanced precursors and cervical cancer survival. PLoS ONE 19(5): e0298118 . [https://doi.org/10.1371/journal.pone.0298118](https://doi.org/10.1371/journal.pone.0298118) [Editor:](Editor: Maria Lina Tornesello)[ Maria Lina Tornesello](Editor: Maria Lina Tornesello), Istituto Nazionale Tumori IRCCS Fondazione Pascale, ITALY Received: August 16, 2023 Accepted: January 16, 2024 Published: May 9, 2024 Copyright: © 2024 Wang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: All relevant data are available within the manuscript and its Supporting Information files. Funding: The author(s) received no specific funding for this work. Competing interests: The authors have declared that no competing interests exist. RESEARCH ARTICLE TPP1 is associated with risk of advanced precursors and cervical cancer survival Qiao-Li Wang1,2☯, Caifeng Gong1,3☯, Xiang-Yu Meng4, Min Fu5, Hui Yang1, Fuxiang Zhou1, Qiuji Wu1 *, Yunfeng Zhou1 * 1 Department of Radiation and Medical Oncology, Hubei Key Laboratory of Tumor Biological Behaviors, Zhongnan Hospital of Wuhan University, Hubei Cancer Clinical Study Center, Wuhan, Hubei, China, 2 Department of Medical Oncology, Dana-Farber Cancer Institute, Boston, MA, United States of America, 3 Department of Medical Oncology, National Cancer Center/National Clinical Research Center for Cancer/ Cancer Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China, 4 Health Science Center, Hubei Minzu University, Enshi, China, 5 Department of Oncology, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China ☯ These authors contributed equally to this work. * [wuqiuji@126.com](wuqiuji@126.com) (QW); [yfzhouwhu@163.com](yfzhouwhu@163.com) (YZ) Abstract It is unclear how telomere-binding protein TPP1 interacts with human telomerase reverse transcriptase (hTERT) and influences cervical cancer development and progression. This study included all eligible 156 cervical cancers diagnosed during 2003–2008 and followed up through 2014, 102 cervical intraepithelial neoplasia (CIN) patients, and 16 participants with normal cervix identified at the same period. Correlation of expression of TPP1 and hTERT in these lesions was assessed using Kappa statistics. TPP1 was knocked down by siRNA in three cervical cancer cell lines. We assessed mRNA expression using quantitative real-time polymerase chain reaction and protein expression using tissue microarray-based immunohistochemical staining. We further analyzed the impact of TPP1 expression on the overall survival of cervical cancer patients by calculating the hazard ratio (HR) with 95% confidence intervals (CIs) using the multivariable-adjusted Cox regression model. Compared to the normal cervix, high TPP1expression was significantly associated with CIN 3 and cervical cancers (P<0.001 for both) . Expressions of TPP1 and hTERT were highly correlated in CIN 3 (Kappa statistics = 0.50, P = 0.005), squamous cell carcinoma (Kappa statistics = 0.22, P = 0.011), and adenocarcinoma/adenosquamous carcinoma (Kappa statistics = 0.77, P = 0.001) . Mechanistically, knockdown of TPP1 inhibited the expression of hTERT in both mRNA and protein levels. High expression of TPP1 (HR = 2.61, 95% CI 1.23–5.51) and cohigh expression of TPP1 and hTERT (HR = 2.38, 95% CI 1.28–4.43) were independently associated with worse survival in cervical cancer patients. TPP1 and hTERT expression was correlated and high expression of TPP1 was associated with high risk of CIN 3 and cervical cancer and could predict a worse survival in cervical cancer. Introdu

FAQs

What is the main research question about TPP1 in cervical cancer?

The study investigates how TPP1 relates to hTERT and whether TPP1 expression influences cervical cancer development, progression, and patient survival.

Which patient and lesion groups were analyzed in the study?

The analysis included 156 cervical cancer cases (2003–2008, followed to 2014), 102 CIN patients, and 16 participants with normal cervix identified in the same period.

How does TPP1 affect hTERT and patient outcomes?

siRNA knockdown of TPP1 inhibited hTERT expression at mRNA and protein levels. High TPP1 expression and co-high TPP1/hTERT expression were independently associated with worse overall survival.

Sep 21, 2026

Upload by Eliana