The impact of 3-sulfo-taurolithocholic acid on ATPase activity in patients' colorectal cancer and normal colon tissues, and its hepatic effects in rodents

Sep 22, 2026

Study tested how 3-sulfotaurolithocholic acid (TLC–S) influences ATPase activities and related ion transport in colorectal cancer versus adjacent healthy colon mucosa, and assessed hepatic effects in rodents. ATPase activity was quantified spectrophotometrically in patient samples (n=10) and mechanistic calcium dynamics were examined in mouse hepatocytes. Rat liver mitochondria were analyzed for respiration and oxidative phosphorylation. TLC–S increased Na+/K+ ATPase in cancer, decreased Mg2+ ATPase in healthy mucosa, altered rat liver Ca2+ and Na+/K+ ATPases, and induced cytosolic Ca2+ signals attenuated by NED-19.

LanguageEnglish

CategoryHealthcare

FormatPDF

Pages12

Reading Time30 min

AccessFree to download

Introduction

Bile acids, secondary bile acids, and colon carcinogenesis

Study hypothesis and investigated ATPase activity

Methods

Patient tissue ATPase measurements

Mouse hepatocyte Ca2+ imaging

Rat liver mitochondria respiration and oxidative phosphorylation

Results

TLC–S effects on Na+/K+ ATPase in cancer vs controls

TLC–S effects on Mg2+ and Ca2+ ATPase activities

TLC–S cytosolic Ca2+ signals and NED-19 attenuation

TLC–S effects on rat liver mitochondrial respiration

Discussion

Differential modulation of ATPase activity in cancerous vs healthy tissues

Mechanistic implications for colorectal cancer and hepatic function

TYPE Original Research PUBLISHED 05 December 2024 DOI 10.3389/fvets.2024.1480122 OPEN ACCESS EDITED BY Xiao-Yu Liu, Southern University of Science and Technology, China REVIEWED BY Mahamane Talphi Diakite, Université des Sciences, des Techniqueset des Technologies de Bamako, Mali Wang Jian, Wuxi Second Geriatric Hospital, China *CORRESPONDENCE Ivan Kushkevych [kushkevych@mail.muni.cz](kushkevych@mail.muni.cz)[ ](kushkevych@mail.muni.cz)Simon K. -M. R. Rittmann [simon.rittmann@univie.ac.at](simon.rittmann@univie.ac.at)[ ](simon.rittmann@univie.ac.at)RECEIVED 14 August 2024 ACCEPTED 31 October 2024 PUBLISHED 05 December 2024 CITATION Bychkova S, Bychkov M, Dordević D, Rittmann SK-MR, Vítězová M and Kushkevych I (2024) The impact of 3-sulfo-taurolithocholic acid on ATPase activity in patients’ colorectal cancer and normal colon tissues, and its hepatic effects in rodents. Front. Vet. Sci. 11:1480122 . doi: 10.3389/fvets.2024.1480122 COPYRIGHT © 2024 Bychkova, Bychkov, Dordević, Rittmann, Vítězová and Kushkevych. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. The impact of 3-sulfo-taurolithocholic acid on ATPase activity in patients’colorectal cancer and normal colon tissues, and its hepatic effects in rodents Solomiia Bychkova 1, Mykola Bychkov 2, Dani Dordević3, Simon K. -M. R. Rittmann4*, Monika Vítězová 5 and Ivan Kushkevych 5* 1 Department of Human and Animal Physiology, Faculty of Biology, Ivan Franko National University of Lviv, Lviv, Ukraine, 2 Department of Therapy No. 1, Medical Diagnostic and Hematology and Transfusiology of Faculty of Postgraduate Education, Danylo Halytsky Lviv National Medical University, Lviv, Ukraine, 3 Department of Plant Origin Food Sciences, Faculty of Veterinary Hygiene and Ecology, University of Veterinary Sciences Brno, Brno, Czechia, 4Archaea Physiology & Biotechnology Group, Department of Functional and Evolutionary Ecology, Universität Wien, Wien, Austria, 5 Department of Experimental Biology, Faculty of Science, Masaryk University, Brno, Czechia Colorectal cancer is influenced by genetic mutations, lifestyle factors, and diet, particularly high fat intake, which raises bile acid levels in the intestinal lumen. This study hypothesized that bile acids contribute to tumorigenesis by disrupting ion transport and ATPase activity in the intestinal mucosa. The effects of 3-sulfotaurolithocholic acid (TLC–S) on ATPase activity were investigated in colorectal cancer samples from 10 patients, using adjacent healthy tissue as controls, and in rodent liver function. ATPase activity was measured spectrophotometrically by determining inorganic phosphorus (Pi) in postmitochondrial fractions. Ca2+ dynamics were assessed in isolated mouse hepatocytes with fluorescence imaging, and rat liver mitochondria were studied using polarographic methods to evaluate respiration and oxidative phosphorylation. TLC–S increased Na+/K+ ATPase activity by 1.5 times in colorectal cancer samples compared to controls (p ≤ 0.05) . In healthy mucosa, TLC–S decreased Mg2+ ATPase activity by 3.6 times (p ≤ 0.05), while Mg2+ ATPase activity in cancer tissue remained unchanged. TLC–S had no significant effect on Ca2+ ATPase activity in healthy colon mucosa but showed a trend toward decreased activity in cancer tissue. In rat liver, TLC–S decreased Ca2+ ATPase and Na+/K+ ATPase activities while increasing basal Mg2+ ATPase activity (p ≤ 0.05) . Additionally, TLC–S induced cytosolic Ca2+ signals in mouse hepatocytes, partially attenuated by NED-19, an NAADP antagonist (p ≤ 0.05) . TLC–S also

FAQs

What was the main hypothesis of the study?

The study hypothesized that bile acids can contribute to tumorigenesis by disrupting ion transport and ATPase activity in the intestinal mucosa.

How did TLC–S affect Na+/K+ ATPase activity in colorectal cancer samples?

TLC–S increased Na+/K+ ATPase activity by about 1.5 times in colorectal cancer samples compared with controls (p ≤ 0.05).

What hepatic and calcium-related effects were observed in rodents?

In rat liver, TLC–S decreased Ca2+ ATPase and Na+/K+ ATPase activities while increasing basal Mg2+ ATPase activity (p ≤ 0.05). In mouse hepatocytes, TLC–S induced cytosolic Ca2+ signals that were partially attenuated by NED-19.

Sep 22, 2026

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