The Efficacy and Mechanism of Qinghua Jianpi Recipe in Inhibiting Canceration of Colorectal Adenoma Based on Inflammatory Cancer Transformation

Sep 24, 2026

Study explores Qinghua Jianpi Recipe for preventing colon polyp recurrence and blocking “inflammatory cancer transformation.” Clinical trials assess effects in inflammatory bowel disease, while an adenoma canceration mouse model evaluates anti-transformation activity. Histopathology, adenoma counts, and ELISA-based inflammatory indices quantify intestinal inflammation and damage. 16S rRNA sequencing and targeted metabolomics characterize microbiota shifts and short-chain fatty acid metabolism, supported by network pharmacology and Western blot signaling pathway validation.

LanguageEnglish

CategoryHealthcare

FormatPDF

Pages19

Reading Time48 min

AccessFree to download

Objective

Methods

Results

Conclusion

Introduction

Hindawi Journal of Immunology Research Volume 2023, Article ID 4319551, 19 pages [https://doi.org/10.1155/2023/4319551](https://doi.org/10.1155/2023/4319551) Research Article The Efficacy and Mechanism of Qinghua Jianpi Recipe in Inhibiting Canceration of Colorectal Adenoma Based on Inflammatory Cancer Transformation Ting Liu , 1 Juping You, 1 Qingjian Gao, 1 Yufeng Zhang , 1 Wei Xu, 1 Desong Kong ,2 Li Chen,3 Bao Yuan , 1 and Haibing Hua 1 1Department of Gastroenterology, Jiangyin Hospital of Traditional Chinese Medicine, Jiangyin Hospital Aﬃliated to Nanjing University of Chinese Medicine, Jiangyin, 214400 Jiangsu Province, China 2Chinese Medicine Modernization and Big Data Research Center, Nanjing Hospital of Chinese Medicine Aﬃliated to Nanjing University of Chinese Medicine, Nanjing University of Chinese Medicine, Nanjing, 210022 Jiangsu Province, China 3Department of Pharmacology, School of Pharmacy, Nanjing University of Chinese Medicine, Nanjing, 210023 Jiangsu Province, China Correspondence should be addressed to Bao Yuan; doctoryuan1985@gmail.com and Haibing Hua; [jyzy3288@163.com](jyzy3288@163.com) Received 16 July 2022; Revised 6 October 2022; Accepted 12 October 2022; Published 15 February 2023 Academic Editor: Xiaojiaoyang Li Copyright © 2023 Ting Liu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Objective. This study is aimed at exploring the eﬀect of Qinghua Jianpi Recipe on preventing colon polyp recurrence and inhibiting the progress of “inﬂammatory cancer transformation.” And another goal is to explore the changes of intestinal ﬂora structure and intestinal inﬂammatory (immune) microenvironment of mice with colon polyps treated by Qinghua Jianpi Recipe and to clarify its mechanism. Methods. Clinical trials were conducted to conﬁrm the therapeutic eﬀect of Qinghua Jianpi Recipe on patients with inﬂammatory bowel disease. The inhibitory eﬀect of Qinghua Jianpi Recipe on “inﬂammatory cancer transformation” of colon cancer was conﬁrmed by an adenoma canceration mouse model. Histopathological examination was used to evaluate the eﬀects of Qinghua Jianpi Recipe on intestinal inﬂammatory state, adenoma number, and pathological changes of adenoma model mice. The changes of inﬂammatory indexes in intestinal tissue were tested by ELISA. Intestinal ﬂora was detected by 16S rRNA high-throughput sequencing. Short-chain fatty acid metabolism in the intestine was analyzed by targeted metabolomics. Network pharmacology analysis of possible mechanism of Qinghua Jianpi Recipe on colorectal cancer was performed. Western blot was used to detect the protein expression of the related signaling pathways. Results. Qinghua Jianpi Recipe can signiﬁcantly improve intestinal inﬂammation status and function in patients withinﬂammatory bowel disease. Qinghua Jianpi Recipe could signiﬁcantly improve the intestinal inﬂammatory activity and pathological damage of adenoma model mice and reduce the number of adenoma. Qinghua Jianpi Recipe signiﬁcantly increased the levels of Peptostreptococcales_Tissierellales, NK4A214_group, Romboutsia, and other intestinal ﬂora after intervention. Meanwhile, the treatment group of Qinghua Jianpi Recipe could reverse the changes of short-chain fatty acids. Network pharmacology analysis and experimental studies showed that Qinghua Jianpi Recipe inhibited the “inﬂammatory cancer transformation” of colon cancer by regulating intestinal barrier function-related proteins, inﬂammatory and immunerelated signaling pathways, and free fatty acid receptor 2 (FFAR2) . Conclusion. Qinghua Jianpi Recipe can improve the intestinal inﬂammatory activity and pathological damage of patient and adenoma cancer model mice. And its mechanism is related to the regulation of intestinal ﬂora structure and abundance, short-chain fatty a

FAQs

What does the study aim to investigate about Qinghua Jianpi Recipe?

It investigates how Qinghua Jianpi Recipe prevents colon polyp recurrence and inhibits the process of “inflammatory cancer transformation.” It also examines changes in intestinal flora structure and the intestinal inflammatory immune microenvironment in mice treated with the recipe.

How were therapeutic effects evaluated in both patients and animal models?

Clinical trials assessed therapeutic effects in inflammatory bowel disease patients. For mechanism and anti-transformation evaluation, an adenoma canceration mouse model was used, with histopathology and adenoma number/pathology scoring.

Which experimental techniques were used to analyze inflammation, microbiota, and metabolism?

Inflammatory indices in tissue were measured using ELISA. Intestinal flora was detected via 16S rRNA high-throughput sequencing, and short-chain fatty acid metabolism was analyzed using targeted metabolomics.

Sep 24, 2026

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