Tetraspanin CD82 Correlates with and May Regulate S100A7 Expression in Oral Cancer

Sep 21, 2026

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Many metastatic cancers with poor prognoses show correlations with downregulated CD82, yet notable exceptions exist, making context essential for CD82’s value as a prognostic biomarker and therapeutic target in oral squamous cell carcinoma (OSCC). The study investigates CD82 function and mechanism in human OSCC cell lines, tissues, and healthy controls using CRISPR-Cas9 knockout, transcriptomics, proteomics, and related assays. CD82 is elevated in CAL 27 cells; CD82 knockout alters over 300 genes and proteins and inhibits cell migration. CD82 expression correlates with S100 proteins in multiple OSCC models and selected patient tissues, with a 37–50 kDa truncated, glycosylated CD82 upregulated in CAL 27. Findings suggest truncated CD82 may contribute to invasive metastasis and poor outcomes, defining a possible OSCC subtype with increased truncated CD82 and S100A7 and supporting CD82 as a prognostic biomarker and therapeutic target.

LanguageEnglish

CategoryResearch & Report

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Pages17

Reading Time43 min

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Abstract

Introduction

Results

CD82 Is Upregulated in CAL 27 Cells

Article Tetraspanin CD82 Correlates with and May Regulate S100A7 Expression in Oral Cancer Kiran Kumar Reddi 1, Weiqiang Zhang 2,3,4, Shokoufeh Shahrabi-Farahani 5, Kenneth Mark Anderson 5, Mingyue Liu 1, David Kakhniashvili 6, Xusheng Wang 7, * and Yanhui H. Zhang 1, * Citation: Reddi, K.K.; Zhang, W.; Shahrabi-Farahani, S.; Anderson, K.M.; Liu, M.; Kakhniashvili, D.; Wang, X.; Zhang, Y.H. Tetraspanin CD82 Correlates with and May Regulate S100A7 Expression in Oral Cancer. Int. J. Mol. Sci. 2024, 25, 2659 . [https://doi.org/10.3390/](https://doi.org/10.3390/)[ ](https://doi.org/10.3390/)ijms25052659 Academic Editor: Zoltán Wiener Received: 21 January 2024 Revised: 18 February 2024 Accepted: 20 February 2024 Published: 24 February 2024 Copyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ([https://](https://)[ ](https://)[creativecommons.org/licenses/by/](creativecommons.org/licenses/by/)[ ](creativecommons.org/licenses/by/)[4.0/](4.0/)) . 1 Department of Bioscience Research, College of Dentistry, University of Tennessee Health Science Center, 875 Union Ave, Memphis, TN 38163, USA 2 Department of Pediatrics, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA 3 Department of Physiology, College of Medicine, University of Tennessee Health Science Center, Memphis, TN 38163, USA 4 USDA-ARS, Pollinator Health in Southern Crop Ecosystem Research Unit, 141 Experiment Station Road, P.O. Box 346, Stoneville, MS 38776, USA 5 Department of Diagnostic Sciences, College of Dentistry, University of Tennessee Health Science Center, 875 Union Ave, Memphis, TN 38163, USA 6 The Proteomics & Metabolomics Core Facility, University of Tennessee Health Science Center, 71 S. Manassas, Suite 110, Memphis, TN 38163, USA 7 Department of Genetics, Genomics & Informatics, University of Tennessee Health Science Center, 71 S. Manassas, Room 410H, Memphis, TN 38163, USA * Correspondence: [xwang39@uthsc.edu](xwang39@uthsc.edu) (X.W.); [yzhang36@uthsc.edu](yzhang36@uthsc.edu) (Y.H.Z.); Tel.: +1-(901)-448-3751 (Y.H.Z.) Abstract: Many metastatic cancers with poor prognoses correlate to downregulated CD82, but exceptions exist. Understanding the context of this correlation is essential to CD82 as a prognostic biomarker and therapeutic target. Oral squamous cell carcinoma (OSCC) constitutes over 90% of oral cancer. We aimed to uncover the function and mechanism of CD82 in OSCC. We investigated CD82 in human OSCC cell lines, tissues, and healthy controls using the CRISPR-Cas9 gene knockout, transcriptomics, proteomics, etc. CD82 expression is elevated in CAL 27 cells. Knockout CD82 altered over 300 genes and proteins and inhibited cell migration. Furthermore, CD82 expression correlates with S100 proteins in CAL 27, CD82KO, SCC-25, and S-G cells and some OSCC tissues. The 37–50 kDa CD82 protein in CAL 27 cells is upregulated, glycosylated, and truncated. CD82 correlates with S100 proteins and may regulate their expression and cell migration. The truncated CD82 explains the invasive metastasis and poor outcome of the CAL 27 donor. OSCC with upregulated truncated CD82 and S100A7 may represent a distinct subtype with a poor prognosis. Differing alternatives from wild-type CD82 may elucidate the contradictory functions and pave the way for CD82 as a prognostic biomarker and therapeutic target. Keywords: oral squamous cell carcinoma; protein expression; gene expression; transcriptomics; oral pathology; proteomics; CRISPR-Cas9; knockout; KAI1; epithelial 1. Introduction Tetraspanin CD82 is a metastasis suppressor. Its expression is often downregulated in advanced stages of many cancers, such as lung, pancreatic, breast, prostate, colon, and ovarian cancers, and this loss of expression is associated with a poor prognosis; however, in leukemia

FAQs

What is the study investigating about CD82 in oral cancer?

The study aims to clarify CD82’s function and mechanism in OSCC and how it may relate to S100A7-associated biology and poor prognosis.

How was CD82 studied in oral squamous cell carcinoma models?

CD82 was examined using human OSCC cell lines and patient tissues, with CRISPR-Cas9 gene knockout plus transcriptomics and proteomics approaches.

What key finding links CD82 to cell behavior and S100 proteins?

CD82 expression correlates with S100 proteins across multiple OSCC models, and CD82 knockout changes many genes and proteins while inhibiting cell migration.

Sep 21, 2026

Uploaded by Finn