Teadenol B as a Component of Microorganism-Fermented Tea Extract Inhibited Breast Cancers by Promoting Autophagy

Sep 21, 2026

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Breast cancer poses a major threat to life and health, requiring safer and more effective therapeutic options. Teadenol B, a characteristic component of microbial fermented tea, shows clear inhibitory effects across four clinical subtype–defined breast cancer cell characteristics. Proteomics indicate that DCTD, a key factor blocking DNA synthesis and repair, is most prominently and consistently reduced. In MDA-MB-231 triple-negative tumor xenografts, teadenol B suppresses tumor growth without significantly altering body weight, and increases LC3-I to LC3-II conversion, supporting autophagy promotion.

LanguageEnglish

CategoryHealthcare

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Pages13

Reading Time33 min

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Introduction

Breast cancer subtype classification

Fermented Pu-erh tea and cancer prevention background

molecules Article Teadenol B as a Component of Microorganism-Fermented Tea Extract Inhibited Breast Cancers by Promoting Autophagy Ying Zhao 1,†, Zhang-Gui Ding 2,3,4,5,6,†, Yu-Jie Yan 1, Rui Yang 2,3,4, Miao-Miao Qi 1, Shu-Kang Pan 2,3,4, Ji-Ling Xie 2,3,4, Yu-Hui Sun 1 and Jin Xiang 1, * Citation: Zhao, Y.; Ding, Z.-G.; Yan, Y.-J.; Yang, R.; Qi, M.-M.; Pan, S.-K.; Xie, J.-L.; Sun, Y.-H.; Xiang, J. Teadenol B as a Component of Microorganism-Fermented Tea Extract Inhibited Breast Cancers by Promoting Autophagy. Molecules 2024, 29, 872. [https://doi.org/10.3390/](https://doi.org/10.3390/)[ ](https://doi.org/10.3390/)molecules29040872 Academic Editors: Stela Dimitrova, Ana M. Dobreva and Petko Denev Received: 18 January 2024 Revised: 9 February 2024 Accepted: 12 February 2024 Published: 16 February 2024 Copyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ([https://](https://)[ ](https://)[creativecommons.org/licenses/by/](creativecommons.org/licenses/by/)[ ](creativecommons.org/licenses/by/)[4.0/](4.0/)) . 1 Ministry of Education Laboratory of Combinatorial Biosynthesis and Drug Discovery, School of Pharmaceutical Science, Wuhan University, Wuhan 430071, China; [zying1305@whu.edu.cn](zying1305@whu.edu.cn) (Y.Z.); [yanyujie524@163.com](yanyujie524@163.com) (Y.-J.Y.); [qmmwhu@126.com](qmmwhu@126.com) (M.-M.Q.); [yhsun@whu.edu.cn](yhsun@whu.edu.cn) (Y.-H.S.) 2 Pu-erh Tea Fermentation Engineering Research Center of Yunnan Province, Kunming 650271, China; [dzg@ynu.edu.cn](dzg@ynu.edu.cn) (Z.-G.D.); [yangrui_xyz@163.com](yangrui_xyz@163.com) (R.Y.); [psk.happy@163.com](psk.happy@163.com) (S.-K.P.); [xiejl520@126.com](xiejl520@126.com) (J.-L.X.) 3 Key Laboratory of Pu-erh Tea Processing Technology, Ministry of Agriculture and Rural Affairs, Kunming 650271, China 4 Yunnan Dayi Microbial Technology Co., Ltd., Kunming 650271, China 5 Yunnan Institute of Microbiology, School of Life Sciences, Yunnan University, Kunming 650091, China 6 Key Laboratory for Southwest Microbial Diversity of the Ministry of Education, Yunnan University, Kunming 650091, China * Correspondence: [xiangjin@whu.edu.cn](xiangjin@whu.edu.cn) † These authors contributed equally to this work. Abstract: Breast cancer is a significant threat to life and health, which needs more safe and effective drugs to be explored. Teadenol B is a characteristic chemical component of microbial fermented tea. This study discovered that teadenol B could exhibit obvious inhibitory effects on all four different clinical subtype characteristics of breast cancer cells. Proteomic studies show that deoxycytidine triphosphate deaminase (DCTD), which could block DNA synthesis and repair DNA damage, had the most significant and consistent reduction in all four types of breast cancer cells with the treatment ofteadenol B. Considering MDA-MB-231 cells exhibit poor clinical prognosis and displayed substantial statistical differences in KEGG pathway enrichment analysis results, we investigated its impact on the size and growth of MDA-MB-231 triple-negative breast tumors transplanted into nude mice and demonstrated that teadenol B significantly suppressed tumor growth without affecting body weight significantly. Finally, we found that the conversion of LC3-I to LC3-II in MDA-MB-231 increased significantly with teadenol B treatment. This proved that teadenol B could be a strong autophagy promotor, which explained the down-regulation of DCTD to some extent and may be the potential mechanism underlying teadenol B’s anti-breast cancer effects. This finding provides new evidence for drinking fermented tea to prevent breast cancer and highlights the potential of teadenol B as a novel therapeutic option for breast cancer prevention and treatment, necessitating further investigations to clarify its exact target and the de

FAQs

What is teadenol B in this study, and what is it tested against?

Teadenol B is a characteristic chemical component of microbial fermented tea. The study tests whether it inhibits breast cancer cells representing multiple clinical subtypes and evaluates effects in MDA-MB-231 triple-negative settings.

How does teadenol B affect molecular markers related to autophagy?

Treatment with teadenol B increases conversion of LC3-I to LC3-II in MDA-MB-231 cells. This supports the conclusion that teadenol B promotes autophagy.

What role does DCTD play in the proposed mechanism?

Proteomic results show the most significant and consistent reduction of DCTD under teadenol B treatment. The study links down-regulation of DCTD to its anti-breast cancer effects, partially explaining the observed inhibition.

Sep 21, 2026

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