SYT7 promotes breast cancer cells growth through the PI3K/AKT pathway

Q: What is the main purpose of this study on SYT7?

To investigate the function and underlying mechanism of synaptotagmin 7 (SYT7) in breast cancer and clarify its connection to prognosis and signaling pathways.

Q: How does SYT7 expression relate to breast cancer prognosis?

High SYT7 expression is associated with a shorter overall survival (OS) and correlates with clinical features such as tumor size and hormone receptor expression levels.

Q: What biological effects does SYT7 have on breast cancer cells?

SYT7 promotes breast cancer cell proliferation and inhibits apoptosis. Knockdown-based inhibition suppresses proliferation and promotes apoptosis.

Sep 22, 2026

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Breast cancer is a highly malignant reproductive-system tumor with poor prognosis. This study investigates the role and underlying mechanism of synaptotagmin 7 (SYT7) in breast cancer, focusing on its relationship with patient outcomes and the PI3K/AKT signaling pathway. Using TCGA and Kaplan-Meier plotter analyses, SYT7 expression is assessed for associations with clinical characteristics and survival. SYT7 knockdown is evaluated by RT-qPCR and Western blotting, while cell proliferation and apoptosis are measured through CCK-8, clone formation, and flow cytometry. Results show SYT7 upregulation in tumors, enhanced proliferation, inhibited apoptosis, and activation of PI3K/AKT signaling, supporting SYT7 as a potential diagnostic and therapeutic target.

LanguageEnglish

CategoryHealthcare

FormatPDF

Pages12

Reading Time30 min

AccessFree to download

Introduction

Breast cancer overview and need for new targets

Methods

TCGA and Kaplan-Meier correlation analysis

SYT7 knockdown and molecular assays

Cell proliferation and apoptosis evaluation

Results

SYT7 upregulation and clinicopathologic correlations

Prognosis association

Functional effects and PI3K/AKT pathway activation

Conclusions

SYT7 as a potential target

SYT7 promotes breast cancer cells growth through the PI3K/AKT pathway Chenghao Luo1,2, Qingfan Mo1,2, Guosheng Ren1,2 1Department of Endocrine and Breast Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China; 2Chongqing Key Laboratory of Molecular Oncology and Epigenetics, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China Contributions: (I) Conception and design: C Luo, G Ren; (II) Administrative support: G Ren; (III) Provision of study materials or patients: C Luo, Q Mo; (IV) Collection and assembly of data: C Luo, Q Mo; (V) Data analysis and interpretation: C Luo, Q Mo; (VI) Manuscript writing: All authors; (VII) Final approval of manuscript: All authors. Correspondence to: Guosheng Ren, MD, PhD. Chongqing Key Laboratory of Molecular Oncology and Epigenetics, the First Affiliated Hospital of Chongqing Medical University, No. 1 Youyi Road, Yuzhong District, Chongqing 40016, China; Department of Endocrine and Breast Surgery, the First Affiliated Hospital of Chongqing Medical University, Chongqing, China. Email: [rengs726@126.com](rengs726@126.com). Background: Breast cancer is one of the most malignant tumors in the reproductive system and has a poor prognosis. The aim of this study was to investigate the function and underlying mechanism of synaptotagmin 7 (SYT7) in breast cancer. Methods: We utilized The Cancer Genome Atlas (TCGA) database and the Kaplan-Meier plotter database to assess the correlation between SYT7 expression and the prognosis of breast cancer patients. The efficacy of SYT7 knockdown was evaluated through reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and Western blotting. Furthermore, we examined the impact of SYT7 on breast cancer cell proliferation and apoptosis using Cell Counting Kit-8 (CCK-8), clone formation assays, and flow cytometry. Through Western blot analysis, we investigated the influence of SYT7 on the expression of apoptosis-related markers and the PI3K/AKT signaling pathway in breast cancer. Results: The TCGA database data analysis revealed a significant up-regulation of SYT7 expression in breast cancer tissues compared to normal tissues (P<0.001) . A correlation was observed between SYT7 expression and tumor size (P=0.009), as well as estrogen receptor (ER) expression level (P<0.001) and progesterone receptor (PR) expression level (P<0.001) in breast cancer patients. Analysis of the KaplanMeier plotter database indicated that high SYT7 expression was associated with a shorter overall survival (OS) (P=0.009) . The mRNA expression results indicated higher SYT7 expression in breast cancer tissues compared to adjacent normal tissues (P=0.005) . CCK-8, clone formation assay, and flow cytometry results demonstrated that SYT7 promoted the proliferation and inhibited the apoptosis of breast cancer cells. Western blot assay confirmed the activation of PI3K/AKT signaling by SYT7 . Conclusions: The findings suggest that SYT7 is highly expressed in breast cancer and that its high expression is linked to clinical characteristics and prognosis. Inhibition of SYT7 through knockdown can suppress proliferation and promote apoptosis of breast cancer cells, making it a potential target for breast cancer diagnosis and treatment. Keywords: Synaptotagmin 7 (SYT7); breast cancer; PI3K/AKT signal; proliferation; apoptosis Submitted Jan 02, 2024. Accepted for publication May 07, 2024. Published online Jun 06, 2024. doi: 10.21037/tcr-24-7 View this article at: [https://dx.doi.org/10.21037/tcr-24-7](https://dx.doi.org/10.21037/tcr-24-7) © Translational Cancer Research. All rights reserved. Transl Cancer Res 2024;13(6):2767-2778 | [https://dx.doi.org/10.21037/tcr-24-7](https://dx.doi.org/10.21037/tcr-24-7) 2768 Luo et al. SYT7 promotes breast cancer cells growth Introduction Breast cancer, a prevalent malignancy among femaleson a global scale, stands as the foremost contributor to cancer-rel

FAQs

What is the main purpose of this study on SYT7?

To investigate the function and underlying mechanism of synaptotagmin 7 (SYT7) in breast cancer and clarify its connection to prognosis and signaling pathways.

How does SYT7 expression relate to breast cancer prognosis?

High SYT7 expression is associated with a shorter overall survival (OS) and correlates with clinical features such as tumor size and hormone receptor expression levels.

What biological effects does SYT7 have on breast cancer cells?

SYT7 promotes breast cancer cell proliferation and inhibits apoptosis. Knockdown-based inhibition suppresses proliferation and promotes apoptosis.

Sep 22, 2026

Upload by Noah