Successful Pregnancy Following Ovarian Platelet-Rich Plasma (PRP) Treatment, the DuoStim Protocol, and Preimplantation Genetic Testing for Aneuploidy - A Case Report

Sep 29, 2026

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Premature ovarian insufficiency (POI) severely limits reproductive options. The report describes a successful live birth in a POI patient after combined intraovarian platelet-rich plasma (PRP) treatment, double ovarian stimulation (DuoStim), and preimplantation genetic testing for aneuploidy (PGT-A). PRP aimed to restore ovarian function, DuoStim to maximize oocyte yield, and embryo testing to select a euploid blastocyst. The transfer resulted in an uncomplicated pregnancy and a healthy child, supporting a personalized multidisciplinary strategy, though additional studies are needed.

LanguageEnglish

CategoryHealthcare

FormatPDF

Pages8

Reading Time20 min

AccessFree to download

Abstract

Introduction

Premature ovarian insufficiency and diagnostic features

Rationale for PRP and stimulation strategies

Case Presentation

Patient and clinical approach

Review began 11/10/2025 Review ended 11/25/2025 Published 12/05/2025 © Copyright 2025 Costanzi et al. This is an open access article distributed under the terms of the Creative Commons Attribution License CCBY 4.0. , which permits unrestricted use , distribution , and reproduction in any medium , provided the original author and source are credited. DOI: 10.7759/cureus.98505 Open Access Case Report Successful Pregnancy Following Ovarian PlateletRich Plasma (PRP) Treatment, the DuoStim Protocol, and Preimplantation Genetic Testing for Aneuploidy in a Patient With Premature Ovarian Insufficiency: A Case Report Flavia Costanzi 1, 2 , Ermanno Greco 1, Sara Fusco 3, Gianluca Dani 3, Giuseppe Grimaldi 3 , Maria Alba Stigliano 4, Ilaria Listorti 1, Pierfrancesco Greco 1 1. Centre for Reproductive Medicine, Villa Mafalda, Rome, ITA 2. Surgical and Medical Sciences and Translational Medicine, Sant 'Andrea Hospital, Sapienza University of Rome, Rome, ITA 3. Centre for Reproductive Medicine, ICSI Roma New Life, Rome, ITA 4. Complex Operational Unit of Immunohematology and Transfusion Medicine (UOCSIMT), Blood Component Production Centre (CPE) Local Health Authority Rome 1 (ASL Roma 1), Rome, ITA Corresponding author: Flavia Costanzi, [costanzi.flavia@gmail.com](costanzi.flavia@gmail.com) Abstract Premature ovarian insufficiency (POI) severely limits reproductive options. We report a successful live birth in a patient with POI following a combined approach of intraovarian platelet-rich plasma (PRP) treatment, double ovarian stimulation (DuoStim protocol), and preimplantation genetic testing for aneuploidy (PGT-A) . PRP treatment was performed to restore ovarian function, after which DuoStim was used to maximize oocyte yield. An embryo obtained underwent PGT-A, and transfer of a euploid blastocyst resulted in an uncomplicated pregnancy and the birth of a healthy child. This case highlights the potential of personalized and multidisciplinary strategies to overcome the traditional barriers in POI by integrating regenerative medicine (PRP), advanced ovarian stimulation (DuoStim), and genetic embryo selection (PGT-A) . While this innovative approach appears promising for women with diminished ovarian reserve, further studies are needed to confirm its efficacy and safety. Categories: Obstetrics/Gynecology, Genetics Keywords: genetic testing for aneuploidy (pgt-a), intraovarian platelet-rich plasma, platelet-rich plasma (prp), poi, premature ovarian insufficiency Introduction Premature ovarian insufficiency (POI) is a complicated and multifaceted disease in reproductive medicine. The condition is defined by loss of ovarian activity before the age of 40 years and is characterized by amenorrhea or irregular menstrual cycles with elevated gonadotropins and low estradiol in accordance with the European Society of Human Reproduction and Embryology (ESHRE) guidelines [1] . The diagnosis is generally made based on amenorrhea, high gonadotropins, and low anti-Müllerian hormone (AMH) levels [1] . Because POI profoundly limits spontaneous fertility and the effectiveness of conventional assisted reproductive technologies (ARTs), it represents a major clinical challenge. Novel attempts have been made in recent years to overcome the scarce reproductive solutions available to women with POI [2] . Intraovarian infusion of platelet-rich plasma (PRP) appears to be a technique that may enable the recovery of ovarian function by promoting high concentrations of growth factors, cytokines, and chemokines in ovarian tissue [3] . These bioactive factors are thought to increase cell proliferation and tissue regeneration and favor a microenvironment for folliculogenesis organization, thereby increasing ovarian parameters [4] . This approach remains experimental but has attracted growing clinical interest. Regarding the type of action, two principal hypotheses have been advanced. The less controversial concept is neo-oogenesis, which i

FAQs

What combined interventions were used in the reported POI case?

The case used intraovarian platelet-rich plasma (PRP) treatment, the DuoStim protocol for double ovarian stimulation, and preimplantation genetic testing for aneuploidy (PGT-A). An embryo underwent PGT-A and a euploid blastocyst was transferred.

How did the report describe the role of PRP in the patient’s treatment?

PRP was performed with the aim to restore ovarian function. The authors describe that PRP may promote ovarian recovery by influencing growth factors and tissue regeneration, enabling improved folliculogenesis.

What was the outcome after using DuoStim and PGT-A?

A euploid blastocyst transfer led to an uncomplicated pregnancy and the birth of a healthy child. The report highlights this as evidence supporting personalized, multidisciplinary strategies for POI.

Sep 29, 2026

Uploaded by Marglet