Spectrophotometric Analysis of Sealing Ability of MTA, Biodentine, and Bio-C Used for Furcal Perforations - An in vitro Study

Q: What was the purpose of this in vitro study?

To evaluate and compare the sealing ability of MTA, Biodentine, and Bio-C Repair when used for furcation perforation repair.

Q: How were the tooth specimens prepared and perforations created?

Thirty human lower first molars were prepared with endodontic access cavities, perforated between the canal orifices, then assigned to three groups and sealed with the respective repair materials.

Q: Which material demonstrated the highest sealing ability and what were the microleakage findings?

Biodentine showed the maximum sealing ability. Mean microleakage values were 1.56 (MTA), 1.23 (Biodentine), and 1.48 (Bio-C Repair), with a significant intergroup difference (P<0.001).

Sep 30, 2026

Uploaded by PakDamar76

Aim: To evaluate and compare the sealing ability of Mineral Trioxide Aggregate (MTA), Biodentine, and Bio-C Repair (a bioceramic-based root repair material) for furcation perforation repair. Materials and Methods: Thirty human lower first molars without caries or restorations were prepared, perforated between canal orifices, and assigned to three groups repaired with MTA, Biodentine, or Bio-C Repair. Specimens were exposed to methylene blue, rinsed, prepared for chemical analysis, centrifuged, and measured for absorbance at 550 nm; one-way ANOVA and post hoc comparisons were used (P<0.05). Results: Mean microleakage was 1.56, 1.23, and 1.48 for Groups I, II, and III respectively, with significant differences (P<0.001). Conclusion: Biodentine showed the maximum sealing ability, followed by Bio-C, then MTA.

LanguageEnglish

CategoryHealthcare

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Pages3

Reading Time8 min

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Abstract

Materials and Methods

Results

Conclusion

Introduction

Root perforations and clinical relevance

Objective of the study

Materials

Original Research Spectrophotometric Analysis of Sealing Ability of MTA, Biodentine, and Bio C Used for Furcal Perforations—An in vitro Study Sathish Abraham, Kshitija M. Diggikar, Pradnya Nagmode, Nitin Lokhande, Vaishnavi A. Deshmukh Department of Conservative Dentistry and Endodontics, SMBT Dental College and Hospital, Sangamner, Ahmednagar, Maharashtra, India Submitted: 05‑Aug‑2025 Revised: 10‑Aug‑2025 Accepted: 18‑Aug‑2025 Published: 17‑Dec‑2025 Abstract Aim: To evaluate and compare the sealing ability of Mineral Trioxide Aggregate, Biodentine, and bioceramic based root repair material Bio‑C Repair when used as afurcation perforation repair material. Materials and Methods: In the present study 30 human lower first molars were used. Collected teeth had no caries or restoration, and none had fused roots. Every molar had an endodontic access cavity made utilizing a high‑speed long—shank round bur for the initial entry and an Endo—Z for lateral extension and finishing the cavity walls. Each canal orifice was covered with a temporary filling material. The pulpal floor and cavity walls of every molar were thoroughly quoted with two successive coats of clear nail polish. A significant perforation was made between the orifices to the formation area using high speed, long shank round bur \#4 . The perforation centered between the medial and distal orifice. They were divided into three experimental groups:‑ Group I: 10 molars repaired using MTA, Group II: 10 molars repaired using Biodentine, Group III: 10 molars repaired using Bio C Repair. Moist cotton pellets were placed over the repair materials, and molars were kept in 100% humidity for 24 h to allow materials to set and later nail varnish was applied to all. Then, according to each group, molars were put in Petri dishes. Methylene blue dye was applied inside the access cavity of all samples for 24 h. Molars were placed under running tap water for 30 min to remove all residues of methylene blue, and then varnish was removed with a Parker blade \#15 and polishing discs. Molars were placed in vials containing 1 mL of concentrated (65 wt%) nitric acid for 3 days. Vials were centrifuged at 14,000 rpm for 5 min. The supernatant from each sample was transferred in a quantity of 200 L to a 96‑well plate. Sample absorbance was read by an automatic microplate spectrophotometer at 550 nm using concentrated nitric acid as a blank. Statistical analysis was performed using one‑way analysis of variance. When the analysis of variance test was significant, the pairwise comparison of the means was done using a post hoc test. The significance level was set at P < 0.05. Statistical analysis was performed with SPSS 14.0 (SPSS Inc., Chicago, IL, USA) for Windows (Microsoft, Redmond, WA, USA) . Results: Mean microleakage seen in Group I: 1.56, Group II: 1.23, Group III: 1.48. Intergroup comparison between Group I, Group II, Group III showed statistically significant difference (P < 0.001) . Conclusion: Within the limitations of the study, it can be concluded that Biodentine showed maximum sealing ability followed by BIO‑C and then MTA. Keywords: Bio C repair, biodentine, furcation, microleakage, MTA, perforation Address for correspondence: Dr. Kshitija M. Diggikar, Department of Conservative Dentistry and Endodontics, SMBT Dental College and Hospital, Sangamner, Ahmednagar, Maharashtra - 422 608, India. E-mail: [drkshitijadiggikar@gmail.com](drkshitijadiggikar@gmail.com) This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License (CC BY-NC-ND), where it is | Access this article online | | | --- | --- | | Quick Response Code:
| Website: [https://journals.lww.com/jpbs](https://journals.lww.com/jpbs) | | | DOI: 10.4103/jpbs.jpbs_1376_25 | permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission fr

FAQs

What was the purpose of this in vitro study?

To evaluate and compare the sealing ability of MTA, Biodentine, and Bio-C Repair when used for furcation perforation repair.

How were the tooth specimens prepared and perforations created?

Thirty human lower first molars were prepared with endodontic access cavities, perforated between the canal orifices, then assigned to three groups and sealed with the respective repair materials.

Which material demonstrated the highest sealing ability and what were the microleakage findings?

Biodentine showed the maximum sealing ability. Mean microleakage values were 1.56 (MTA), 1.23 (Biodentine), and 1.48 (Bio-C Repair), with a significant intergroup difference (P<0.001).

Sep 30, 2026

Uploaded by PakDamar76