Specific Deletions of Chromosomes 3p, 5q, 13q, and 21q among Patients with G2 Grade of Non-Small Cell Lung Cancer - Case Report

Sep 21, 2026

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Non-small cell lung cancer (NSCLC) causes substantial cancer-related premature mortality in Western populations. This case report applies DNA microarray-based gene-expression profiling to molecularly characterize NSCLC specimens and uncover tumor-specific genomic alterations. The study focuses on chromosomal copy-number changes, identifying recurrent loss of regions 3p, 5q, 13q, and 21q, alongside gain of 12p. Metascape-driven bioinformatic analysis highlights disrupted pathways that may inform early detection, prognostication, and future molecular-targeted therapies.

LanguageEnglish

CategoryResearch & Report

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Pages18

Reading Time45 min

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Abstract

Keywords

1. Introduction

Lung cancer burden and risk factors

Classification of lung cancer and genomic complexity

Case Report Specific Deletions of Chromosomes 3p, 5q, 13q, and 21q among Patients with G2 Grade of Non-Small Cell Lung Cancer Agata Kolecka-Bednarczyk 1, *,†, Magdalena Frydrychowicz 1,†, Bartłomiej Budny 2, Marcin Ruci ´nski 3, Claudia Dompe 1,4, Piotr Gabryel 5, Bartosz J. Płachno 6, *, Marek Ruchała 2, Katarzyna Ziemnicka 2, Paweł Zieli ´nski 5 and Joanna Budna-Tukan 3,7 Citation: Kolecka-Bednarczyk, A.; Frydrychowicz, M.; Budny, B.; Ruci ´nski, M.; Dompe, C.; Gabryel, P.; Płachno, B.J.; Ruchała, M.; Ziemnicka, K.; Zieli ´nski, P.; et al. Specific Deletions of Chromosomes 3p, 5q, 13q, and 21q among Patients with G2 Grade of Non-Small Cell Lung Cancer. Int. J. Mol. Sci. 2024, 25, 8642. [https://](https://)[ ](https://)[doi.org/10.3390/ijms25168642](doi.org/10.3390/ijms25168642) Academic Editors: Anna Cie´sli ´nska, Ewa Fiedorowicz and Barbara Ruaro Received: 7 May 2024 Revised: 3 August 2024 Accepted: 5 August 2024 Published: 8 August 2024 Copyright: © 2024 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ([https://](https://)[ ](https://)[creativecommons.org/licenses/by/](creativecommons.org/licenses/by/)[ ](creativecommons.org/licenses/by/)[4.0/](4.0/)) . 1 Department of Immunology, Poznan University of Medical Sciences, 60-806 Poznan, Poland; [frydrychowicz@ump.edu.pl](frydrychowicz@ump.edu.pl) (M.F.); [84416@student.ump.edu.pl](84416@student.ump.edu.pl) (C.D.) 2 Department of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences, 60-355 Poznan, Poland; [bbudny@ump.edu.pl](bbudny@ump.edu.pl) (B.B.); [mruchala@ump.edu.pl](mruchala@ump.edu.pl) (M.R.); [kaziem@ump.edu.pl](kaziem@ump.edu.pl) (K.Z.) 3 Department of Histology and Embryology, Poznan University of Medical Sciences, 60-781 Poznan, Poland; [marcinruc@ump.edu.pl](marcinruc@ump.edu.pl) (M.R.); [jbudna@ump.edu.pl](jbudna@ump.edu.pl) (J.B.-T.) 4 Doctoral School, Poznan University of Medical Sciences, 60-812 Poznan, Poland 5 Department of Thoracic Surgery, Poznan University of Medical Sciences, 60-569 Poznan, Poland; [pgabryel@ump.edu.pl](pgabryel@ump.edu.pl) (P.G.); [pzielinski986@gmail.com](pzielinski986@gmail.com) (P.Z.) 6 Department of Plant Cytology and Embryology, Institute of Botany, Faculty of Biology, Jagiellonian University in Kraków, 30-387 Cracow, Poland 7 Department of Anatomy and Histology, Collegium Medicum, University of Zielona Gora, 65-046 Zielona Gora, Poland * Correspondence: [akolb@ump.edu.pl](akolb@ump.edu.pl) (A.K.-B.); [bartosz.plachno@uj.edu.pl](bartosz.plachno@uj.edu.pl) (B.J.P.)† These authors contributed equally to this work. Abstract: Non-small cell lung cancer (NSCLC) leads as a primary cause of cancer-related premature mortality in Western populations. This study leverages cutting-edge gene-expression-profiling technologies to perform an in-depth molecular characterization of NSCLC specimens, with the objective of uncovering tumor-specific genomic alterations. By employing DNA microarray analysis, our research aims to refine the classification of NSCLC for early detection, guide molecular-targeted treatment approaches, enhance prognostication, and broaden the scientific understanding of the disease’s biology. We identified widespread genomic abnormalities in our samples, including therecurrent loss of chromosomal regions 3p, 5q, 13q, and 21q and the gain of 12p. Furthermore, utilizing Metascape for bioinformatic analysis revealed critical biological pathways disrupted in NSCLC, offering promising leads for novel therapeutic interventions. Keywords: lung cancer; microarrays; loss of heterozygosity; gene enrichment 1. Introduction Lung cancer is the most common neoplasm in both men and women. In 2018, lung cancer was globally the most diagnosed cancer, with 2,094,000 new cases. It ranks second in both men and women, with 1,369,000 a

FAQs

What genomic changes are reported in patients with G2 grade NSCLC?

The study reports recurrent loss of chromosomal regions 3p, 5q, 13q, and 21q, as well as gain of 12p in the analyzed samples.

What methods were used to characterize NSCLC molecularly?

DNA microarray analysis was used for gene-expression-profiling and molecular characterization, followed by Metascape-based bioinformatic pathway analysis.

Why is this research relevant for clinical management of NSCLC?

By identifying tumor-specific genomic alterations and disrupted pathways, the work aims to support refined classification, early detection, improved prognostication, and better selection of molecular-targeted treatment approaches.

Sep 21, 2026

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