Redox proteomics of PANC-1 cells reveals the significance of HIF-1 signaling protein oxidation in pancreatic ductal adenocarcinoma pathogenesis

Sep 21, 2026

Uploaded by Finn

Protein cysteine oxidation contributes to diverse biological and pathogenic processes, yet its role in pancreatic cancer development remains insufficiently defined. The study applies iodoTMT-based quantitative proteomics to map protein oxidation in PDAC cells, revealing 4300+ oxidized cysteine sites across 2100+ proteins and 1715 residues differentially oxidized between HPDE6c7 and PANC-1. Oxidized regions show enrichment of charged residues, with PANC-1 selectively affecting cancer pathways. Notably, HIF-1 signaling proteins display marked oxidation changes, and reduced PHD2 oxidation in patient tissues correlates with shorter survival.

LanguageEnglish

CategoryResearch & Report

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Pages16

Reading Time40 min

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Abstract

Introduction

Background of pancreatic cancer and PDAC prognosis

Rationale for studying protein cysteine oxidation

Tan et al. Journal of Translational Medicine (2024) 22:287 [https://doi.org/10.1186/s12967-024-05068-z](https://doi.org/10.1186/s12967-024-05068-z) Journal of Translational Medicine RESEARCH Open Access Redox proteomics of PANC-1 cells reveals the significance of HIF-1 signaling protein oxidation in pancreatic ductal adenocarcinoma pathogenesis Chaochao Tan1,2, Lichun Chen3, Xiaoyu Guan3, Wenyi Huang3, Yinhong Feng3, Ziyi Li3, Ling Wu1, Xiangping Huang1, Qianhui Ouyang1, Sixiang Liu4, Ying Huang4* and Jiliang Hu3,5* Abstract Background Protein cysteine oxidation is substantially involved in various biological and pathogenic processes, but its implications in pancreatic cancer development remains poorly understood. Methods and results In this study, we performed a global characterization of protein oxidation targets in PDAC cells through iodoTMT-based quantitative proteomics, which identified over 4300 oxidized cysteine sites in more than 2100 proteins in HPDE6c7 and PANC-1 cells. Among them, 1715 cysteine residues were shown to be differentially oxidized between HPDE6c7 and PANC-1 cells. Also, charged amino acids including aspartate, glutamate and lysine were significantly overrepresented in flanking sequences of oxidized cysteines. Differentially oxidized proteins in PANC-1 cells were enriched in multiple cancer-related biological processes and signaling pathways. Specifically, the HIF-1 signaling proteins exhibited significant oxidation alterations in PANC-1 cells, and the reduced PHD2 oxidation in human PDAC tissues was correlated with lower survival time in pancreatic cancer patients. Conclusion These investigations provided new insights into protein oxidation-regulated signaling and bio logical processes during PDAC pathogenesis, which might be further explored for pancreatic cancer diagnosis and treatment. Keywords Protein cysteine oxidation, iodoTMT, Redox proteomics, Pancreatic ductal adenocarcinoma, PANC-1 *Correspondence: Ying Huang [carmen_huang@163.com](carmen_huang@163.com)[ ](carmen_huang@163.com)Jiliang Hu [jlhu@cqmu.edu.cn](jlhu@cqmu.edu.cn) 1 Department of Clinical Laboratory, Hunan Provincial People’s Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha 410005, China 2 Tumor Immunity Research Center of Hunan Provincial Geriatric Institute, Hunan Provincial People’s Hospital (The First Affiliated Hospital of Hunan Normal University), Changsha 410005, China 3 School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China 4 Department of Emergency, The First Affiliated Hospital of Hunan Normal University (Hunan Provincial People’s Hospital), Changsha 410006, Hunan, China 5 Institute of Life Sciences, Chongqing Medical University, Chongqing 400032, China © The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit [http://creativecommons.org/licenses/by/4.0/](http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver ()[. The Creative Commons Public Domain Dedication waiver (](http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver ()[http://creativeco](http://creativeco)[mmons.o

FAQs

What cellular process does the study focus on in pancreatic cancer?

The study focuses on protein cysteine oxidation, particularly how redox modifications influence signaling and pathogenic processes in pancreatic ductal adenocarcinoma (PDAC).

How were oxidized cysteine targets identified in PDAC cells?

IodoTMT-based quantitative proteomics was used to globally characterize protein oxidation targets in PDAC cell lines, identifying thousands of oxidized cysteine sites across many proteins.

Which signaling pathway is highlighted by the oxidation results?

HIF-1 signaling proteins show significant oxidation alterations in PANC-1 cells, and reduced PHD2 oxidation in PDAC tissues is associated with poorer patient survival.

Sep 21, 2026

Uploaded by Finn