QIAGEN Plasmid Purification Handbook - Plasmid Mini, Midi, Maxi, Mega, and Giga Kits - For purification of ultrapure, transfection-grade plasmid DNA

Sep 27, 2026

Uploaded by cksankcs

QIAGEN Plasmid Purification Handbook provides guidance for purifying ultrapure, transfection-grade plasmid DNA using QIAGEN Plasmid Mini, Midi, Maxi, Mega, and Giga kits. It outlines kit contents, shipping and storage requirements, intended use for molecular biology applications, and key safety information. The handbook includes quality control, the underlying principle and procedure, required equipment and reagents, important notes, detailed purification protocols for different kit formats, troubleshooting, and appendices for agarose gel analysis and buffer compositions.

LanguageEnglish

CategoryHealthcare

FormatPDF

Pages68

Reading Time2 h 51 min

AccessFree to download

Contents

Kit Contents

Shipping and Storage

Intended Use

Safety Information

Quality Control

Introduction

Principle and procedure

Equipment and Reagents to Be Supplied by User

Important Notes

Protocol: Plasmid or Cosmid DNA Purification Using QIAGEN Plasmid Mini Kit

Protocol: Plasmid or Cosmid DNA Purification Using QIAGEN Plasmid Midi and Maxi Kits

Protocol: Plasmid or Cosmid DNA Purification Using QIAGEN Plasmid Mega and Giga Kits

Protocol: Very Low-Copy Plasmid/Cosmid Purification Using QIAGEN-tip 100 or QIAGEN-tip 500

Troubleshooting Guide

Appendix A: Agarose Gel Analysis of the Purification Procedure

Appendix B: Composition of Buffers

Ordering Information

Document Revision History

July 2023 QIAGEN® Plasmid Purification Handbook QIAGEN Plasmid Mini, Midi, Maxi, Mega, and Giga Kits For purification of ultrapure, transfection-grade plasmid DNA Contents Kit Contents................................................................................................................ 3 Shipping and Storage ................................................................................................. 5 Intended Use .............................................................................................................. 5 Safety Information ....................................................................................................... 6 Quality Control........................................................................................................... 6 Introduction ................................................................................................................ 7 Principle and procedure .................................................................................... 7 Equipment and Reagents to Be Supplied by User ............................................................ 9 Important Notes ........................................................................................................ 10 Protocol: Plasmid or Cosmid DNA Purification Using QIAGEN Plasmid Mini Kit .............. 18 Protocol: Plasmid or Cosmid DNA Purification Using QIAGEN Plasmid Midi and Maxi Kits.............................................................................................................................. 24 Protocol: Plasmid or Cosmid DNA Purification Using QIAGEN Plasmid Mega and Giga Kits.............................................................................................................................. 31 Protocol: Very Low-Copy Plasmid/Cosmid Purification Using QIAGEN-tip 100 or QIAGEN-tip 500 ...................................................................................................... 40 Troubleshooting Guide .............................................................................................. 48 Appendix A: Agarose Gel Analysis of the Purification Procedure .................................... 54 Appendix B: Composition of Buffers ............................................................................ 58 Ordering Information ................................................................................................ 61 Document Revision History ......................................................................................... 66 Kit Contents QIAGEN Plasmid Kit Catalog no. Mini (25) 12123 Mini (100) 12125 Midi (25) 12143 Midi (100) 12145 Maxi (10) 12162 Maxi (25) 12163 Maxi (100) 12165 QIAGEN-tip 20 QIAGEN-tip 500 Buffer P2 Buffer QBT Buffer QF LyseBlue® 25 – 20 mL 60 mL 30 mL 20 µL 100 – 2 x 20 mL 2 x 60 mL 140 mL 2 x 20 µL – – 110 mL 2 x 60 mL 200 mL 110 µL – – 500 mL 500 mL 4 x 140 mL 500 µL – 10 110 mL 2 x 60 mL 200 mL 110 µL – 25 2 x 150 mL 2 x 200 mL 510 mL 2 x 150 µL – – 4 x 25 8 x 150 mL 8 x 150 mL 8 x 200 mL 4 x 510 mL 8 x 15 mg 8 x 150 µL QIAGEN-tip 100 – – 25 100 – – Buffer P1 20 mL 2 x 20 mL 110 mL 500 mL 110 mL 2 x 150 mL 8 x 150 mL Buffer P3 20 mL 2 x 20 mL 110 mL 500 mL 110 mL 2 x 150 mL Buffer QC 2 x 60 mL 2 x 240 mL 3 x 205 mL 5 x 500 mL 3 x 240 mL 4 x 500 mL 16 x 500 mL RNase A* 2 mg 2 x 2 mg 11 mg 50 mg 11 mg 2 x 15 mg Quick-Start Protocol 1 1 1 1 1 1 1 * Provided in a 10 mg/mL or 100 mg/mL solution. QIAGEN Plasmid Kit Catalog no. Mega (5) 12181 Mega (25) 12183 Giga (5) 12191 Plasmid Buffer Set 19046 QIAGEN-tip 2500 5 25 – – | QIAGEN-tip 10000 | – | – | 5 | – | | --- | --- | --- | --- | --- | | Buffer P1 | 2 x 150 mL | 3 x 500 mL | 3 x 25

FAQs

What are QIAGEN Plasmid Kits intended for?

They are intended for molecular biology applications, not for diagnosis, prevention, or treatment of a disease.

How should QIAGEN tips and kit components be stored?

QIAGEN tips should be stored dry at room temperature (15–25°C). Plasmid kit components are stored at room temperature, while after adding RNase A, Buffer P1 should be stored at 2–8°C.

Which sections help users follow purification protocols and resolve issues?

The handbook provides detailed protocols for plasmid/cosmid purification and a Troubleshooting Guide, with additional appendices for agarose gel analysis and buffer composition.

Sep 27, 2026

Uploaded by cksankcs