Pectolinarigenin regulates the tumor-associated proteins in AGS-xenograft BALB/c nude mice

Sep 21, 2026

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Pectolinarigenin (PEC), a flavone extracted from Cirsium with anti-inflammatory properties, is investigated for its role in tumor control and pathway regulation. AGS cells were xenografted into BALB/c nude mice, with PEC administered intraperitoneally to assess effects on tumor development. After animal testing, tumor proteins were isolated and analyzed by LC–MS, followed by gene ontology analysis of identified proteins. PEC was not harmful to non-cancerous cells and regulated 6 of 582 tumor-associated proteins in vivo and in vitro.

LanguageEnglish

CategoryResearch & Report

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Pages12

Reading Time30 min

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Abstract

Background

Methods

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Introduction

Molecular Biology Reports (2024) 51:305 [https://doi.org/10.1007/s1](https://doi.org/10.1007/s1) 1033-023-09046-4 Pectolinarigenin regulates the tumor-associated proteins in AGSxenograft BALB/c nude mice Ho Jeong Lee1 · Young Sang Kwon2 · Ju Hong Lee1 · Yeon Gyu Moon1 · Jungil Choi1 · Moonjung Hyun1 · Tae KilTak1 · Je‑Hein Kim1 · Jeong Doo Heo1 Received: 7 March 2023 / Accepted: 30 October 2023 / Published online: 16 February 2024 © The Author(s) 2024 Abstract Background Pectolinarigenin (PEC) is a flavone extracted from Cirsium, and because it has anti-inflammatory properties, anti-cancer research is also being conducted. The objective of this work was to find out if PEC is involved in tumor control and which pathways it regulates in vivo and in vitro. Methods AGS cell lines were xenografted into BALB/c nude mice to create tumors, and PEC was administered intraperitoneally to see if it was involved in tumor control. Once animal testing was completed, tumor proteins were isolated and identified using LC–MS analysis, and gene ontology of the found proteins was performed. Results Body weight and hematological measurements on the xenograft mice model demonstrated that PEC was not harmful to non-cancerous cells. We found 582 proteins in tumor tissue linked to biological reactions such as carcinogenesis and cell death signaling. PEC regulated 6 out of 582 proteins in vivo and in vitro in the same way. Conclusion Our findings suggested that PEC therapy may inhibit tumor development in gastric cancer (GC), and proteomic research gives fundamental information about proteins that may have great promise as new therapeutic targets in GC. Keywords Pectolinarigenin · AGS-xenograft · Proteomics · Tumor suppress · PI3K/AKT/mTOR Introduction Cancer is well known around the world as a disease that endangers human life, and research is being performed to produce new medications for treating cancer and improving environmental conditions. Cancer, often known as a malignant tumor, is a condition in which the cell cycle and cell division are uncontrolled [1] . Gastric cancer (GC) has the second-highest global incidence and is common throughout East Asia, particularly Korea [2] . According to recent research, more than half of GC cases are linked to alcohol intake, smoking, infections, food, and obesity [3, 4]. Although different research are being undertaken to uncover potential therapies for GC, early identification remains difficult, and it has a high recurrence rate following surgery; GC is also one of the most difficult malignancies to cure due to chemotherapy side effects [5] . Anticancer medications with low side effects that do not impact healthy cells are required to overcome these obstacles. * Jeong Doo Heo [jdher@kitox.re.kr](jdher@kitox.re.kr) Ho Jeong Lee [hojeong.lee@kitox.re.kr](hojeong.lee@kitox.re.kr)[ ](hojeong.lee@kitox.re.kr)Young Sang Kwon [youngsang.kwon@kitox.re.kr](youngsang.kwon@kitox.re.kr)[ ](youngsang.kwon@kitox.re.kr)Ju Hong Lee [juhonglee@kitox.re.kr](juhonglee@kitox.re.kr) Yeon Gyu Moon [ygmoon@kitox.re.kr](ygmoon@kitox.re.kr) Jungil Choi [jungil.choi@kitox.re.kr](jungil.choi@kitox.re.kr) Moonjung Hyun [moonjung.hyun@kitox.re.kr](moonjung.hyun@kitox.re.kr) Tae Kil Tak [ttkil@kitox.re.kr](ttkil@kitox.re.kr) [Je-Hein Kim](Je-Hein Kim) [jehein@kitox.re.kr](jehein@kitox.re.kr) 1 Gyeongnam Bio-Health Research Support Center, Gyeongnam Branch Institute, Korea Institute of Toxicology (KIT), 17 Jeigok-gil, Jinju 52834, Republic of Korea 2 Environmental Safety Assessment Center, Gyeongnam Branch Institute, Korea Institute of Toxicology (KIT), 17 Jeigok-gil, Jinju 52834, Republic of Korea The development of medicines based on natural ingredients has gained popularity. We previously investigated the efficacy natural flavonoid active components in killing cancer cells indifferent malignancies, and we recently investigated the efficacy ofpectolinarigenin (PEC) in GC cell lines

FAQs

What is the main objective of studying Pectolinarigenin (PEC)?

To determine whether PEC is involved in tumor control and to identify which pathways it regulates in vivo and in vitro.

How was the in vivo tumor model created and how was PEC delivered?

AGS cells were xenografted into BALB/c nude mice to form tumors, and PEC was administered intraperitoneally.

What proteomics results support PEC’s effect on tumors?

Proteomic analysis identified 582 proteins in tumor tissue linked to carcinogenesis and cell death signaling, and PEC regulated 6 of these proteins similarly in vivo and in vitro.

Sep 21, 2026

Uploaded by Himbo