Overexpression of Transmembrane Phosphatase with Tensin homology (TPTE) in prostate cancer is clinically significant

Q: What role does TPTE play in prostate cancer according to the study?

TPTE is a CTA family member expressed in healthy testis and is overexpressed in prostate cancer tissues. The study links higher TPTE levels with disease progression.

Q: How was TPTE expression measured in prostate cancer tissues?

Polyclonal antibodies against TPTE-p1 and TPTE-p2 peptides were produced. Anti-TPTE-p2 was applied to 102 PCa and 48 BPH tissue samples using immunohistochemistry.

Q: What effects did anti-TPTE-p2 have on cancer cell lines?

Anti-TPTE-p2 inhibited proliferation of PC-3 and MCF-7 cells in comparison with controls at both 24 and 48 hours in the MTT test.

Sep 22, 2026

Upload by Olivia Brown

Cancer testis antigens (CTAs) are proteins typically present in male testis but abnormally overexpressed in multiple cancer types. This study investigates transmembrane phosphatase with tensin homology (TPTE) as a CTA and evaluates TPTE expression in prostate cancer tissues. A novel anti-TPTE antibody marker was generated using extracellular TPTE peptides, followed by immunohistochemistry on 102 PCa and 48 BPH samples and MTT assays on PC-3 and MCF-7 cells.

LanguageEnglish

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Journal of Cancer Research and Clinical Oncology (2024) 150:165 [https://doi.org/10.1007/s00432-024-05694-6](https://doi.org/10.1007/s00432-024-05694-6) Overexpression of Transmembrane Phosphatase with Tensin homology (TPTE) in prostate cancer is clinically significant, suggesting its potential as a valuable biomarker Nahid Zainodini1 · Maryam Abolhasani2,3 · Monireh Mohsenzadegan4 · Mohammad M. Farajollahi1 · Elham Rismani5 Received: 18 January 2024 / Accepted: 8 March 2024 / Published online: 28 March 2024 © The Author(s) 2024 Abstract Purpose Cancer testis antigens (CTAs) are a family of proteins typically expressed in male testicles but overexpressed in various cancer cell types. Transmembrane Phosphatase with Tensin homology (TPTE) is expressed only in the testis of healthy individuals and is a member of the family of CTAs. The current study, for the first time, examined the significance of TPTE expression in prostate cancer (PCa) tissues by generating a novel antibody marker targeting TPTE protein. Methods Polyclonal antibodies were prepared for TPTE-p1 and TPTE-p2 peptides, which are derived from the extracellular domains ofTPTE. Anti-TPTE-p2 antibody was then used to study the extent and pattern ofTPTE expression in 102 PCa and 48 benign prostatic hyperplasia (BPH) tissue samples by immunohistochemistry. The viability of cancer cell lines (PC-3 and MCF-7 cells) was also evaluated in the presence of anti-TPTE-p2 antibody using the MTT test. Results The immunohistochemical analysis demonstrated a significant increase in cytoplasmic and membrane TPTE expression in the PCa samples compared to the BPH group (both P < 0.0001) . Cytoplasmic TPTE expression was positively correlated with Gleason score and PSA levels (P = 0.03 and P = 0.001, respectively) . Significant correlations were identified between the levels of PSA and perineural invasion and the membrane expression (P = 0.01, P = 0.04, respectively). Moreover, anti-TPTE-p2 antibody inhibited PC-3 and MCF-7 cells proliferation compared to the control group for 24 h (P < 0.001 and P = 0.001, respectively) as well as for 48 h (P = 0.001 and P = 0.001, respectively) . Conclusion Our findings indicate that increased TPTE expression is associated with progression of disease. The ability of anti-TPTE-p2 antibody to recognize and target the TPTE protein makes it a potential biomarker to assess and/or target the PCa. Keywords TPTE protein · Prostate cancer (PCa) · Immunohistochemistry · Benign prostatic hyperplasia (BPH) · Targeted therapy * Monireh Mohsenzadegan [mohsenzadegan.m@iums.ac.ir](mohsenzadegan.m@iums.ac.ir); [monirehmohsenzadegan@gmail.com](monirehmohsenzadegan@gmail.com) * Mohammad M. Farajollahi [fam-biotec@iums.ac.ir](fam-biotec@iums.ac.ir); [m.farajollahi@gmail.com](m.farajollahi@gmail.com) 1 Department of Medical Biotechnology, School of Allied Medical Sciences, Iran University of Medical Sciences (IUMS), Hemmat Highway, Tehran, Iran 2 Department of Pathology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran 3 Hasheminejad Kidney Center, Iran University of Medical Sciences, Tehran, Iran 4 Department of Medical Laboratory Sciences, Faculty of Allied Medical Sciences, Iran University of Medical Sciences (IUMS), Hemmat Highway, Tehran, Iran 5 Molecular Medicine Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran Introduction Prostate cancer (PCa), the second most frequent cancer among men, is a heterogeneous disease and the fifth cause of death in men (D’Elia et al. 2022 ; Torre et al. 2016) . Approximately 1,414,259 people were diagnosed with PCa in 2020, and 375,304 of them died. This number will almost double to 2.3 million cases and 740,000 deaths in 2040 worldwide (Chung et al. 2019 ; Culp et al. 2020 ; Ferlay et al. 2019 ; Sung et al. 2021) . While prostate-specific antigen (PSA)-based screening for PCa has had a beneficial effect in reducing PCa mortality, it has also

FAQs

What role does TPTE play in prostate cancer according to the study?

TPTE is a CTA family member expressed in healthy testis and is overexpressed in prostate cancer tissues. The study links higher TPTE levels with disease progression.

How was TPTE expression measured in prostate cancer tissues?

Polyclonal antibodies against TPTE-p1 and TPTE-p2 peptides were produced. Anti-TPTE-p2 was applied to 102 PCa and 48 BPH tissue samples using immunohistochemistry.

What effects did anti-TPTE-p2 have on cancer cell lines?

Anti-TPTE-p2 inhibited proliferation of PC-3 and MCF-7 cells in comparison with controls at both 24 and 48 hours in the MTT test.

Sep 22, 2026

Upload by Olivia Brown