Overexpression of miR-195-5p Suppresses Gastric Cancer Progression by Regulating LAMP2-Mediated Autophagy

Sep 23, 2026

MiR-195-5p is linked to cancer development, but its role in gastric cancer (GC) is unclear. This study evaluated miR-195-5p expression in clinical specimens and TCGA data, then assessed GC cell phenotypes using CCK-8, clone formation, flow cytometry, transwell and wound-healing assays. Mechanistic work combined KEGG and database analyses with Western blot and rescue experiments. Results show miR-195-5p is reduced in GC and suppresses growth, metastasis, and promotes apoptosis, acting through LAMP2-mediated autophagy.

LanguageEnglish

CategoryHealthcare

FormatPDF

Pages16

Reading Time40 min

AccessFree to download

Abstract

Keywords

Introduction

Biochemical Genetics (2026) 64:1983–1998 [https://doi.org/10.1007/s10528-025-1](https://doi.org/10.1007/s10528-025-1)1120-4 ORIGINAL ARTICLE Overexpression of miR‑195‑5p Suppresses Gastric Cancer Progression by Regulating LAMP2‑Mediated Autophagy Chenglong Shen1 · Fang Zhou1 · Xiaolei Zhang2 · Jiazhe Shao1 · Yuchao Wang1 · Haichen Liu1 · Yichen Li1 · Guoqiang Zhou1 · Zhiliang Shi1 Received: 9 March 2025 / Accepted: 22 April 2025 / Published online: 5 May 2025 © The Author(s) 2025 Abstract MiR-195-5p has been confirmed to be intimately linked to many cancers development, whereas its role in gastric cancer (GC) remains unelucidated. The objective of this study was to explore the potential function and mechanism of miR-195-5pin GC. First, miR-195-5p level in GC was analyzed based on clinical specimens and TCGA database. Then, the function of miR-195-5p in GC cells was detected via CCK-8, clone formation assay, flow cytometry, transwell, and wound healing assay. Subsequently, we explored the possible mechanism of miR-195-5p using KEGG analysis, database analysis, Western blot, and rescue assays. In this research, miR-195-5p was declined in GC and associated to malignant progression of GC. The introduction of miR-195-5p weakened cell growth, metastasis, and facilitated cell apoptosis. KEGG enrichment analysis indicated that miR-195-5p influenced the malignant progression of GC through mechanisms related to autophagy. MiR- 195-5p overexpression leads to a diminution in cellular autophagy. Further studies identified that miR-195-5p bound to Lysosome-associated membrane protein 2 (LAMP2) . Mechanism studies confirmed that miR-195-5p introduction attenuated cell growth, and this process was rescued by LAMP2 overexpression. Collectively, our work disclosed that miR-195-5p affected the malignant progression of GC by regulating LAMP2-mediated autophagy. Keywords Gastric cancer · MiR-195-5p · LAMP2 · Autophagy · Malignant progression Chenglong Shen, Fang Zhou and Xiaolei Zhang have contributed equally to this work. Extended author information available on the last page of the article Introduction Gastric cancer (GC) is recognized as a frequent form of cancer in the digestive system. Based on the analysis of statistics in 2022, saw an estimated 970,000 new cases of GC and 660,000 fatalities worldwide (Bray et al. 2024) . Several factors contribute to the occurrence of GC, including late-night habits, an unbalanced diet, alcohol consumption, and Helicobacter pylori infection (Machlowska et al. 2020) . In recent years, human beings have made remarkable achievements in the fields of chemotherapy, surgical treatment and molecular targeted therapy for GC. With the progress of technology, the incidence rate and mortality of GC have reduced (Song et al. 2017) . However, GC often has an insidious onset, which means most patients are diagnosed at advanced stages. This increases their likelihood of recurrence and metastasis after surgery, resulting in a poor prognosis and a 5-year survival rate of around 20%(Díaz Del Arco et al. 2024) . Therefore, exploring the mechanisms of GC development and searching for specific markers have become important issues that need to be addressed urgently. The microRNAs (miRNAs) constitute a category of endogenous, non-coding RNAs, characterized by their high degree of conservation and an average length of around 22 nucleotides. miRNAs act as negative regulators of gene expression through binding to the 3 ′ UTR of messenger RNAs (mRNAs), hindering the translation process or enhancing the degradation of these mRNAs (Bartel 2009) . Research have reported that miRNAs act a vital role in regulating tumorigenesis and development. For instance, miR-339-5p weakens the migration and proliferation of GC cells via ALKBH1 (Wang et al. 2020) . Jiang et al. found that miRNA- 15a-3p expression was lower in hepatocellular carcinoma tissues. Patients with reduced levels of miRNA-15a-3p had higher rates of met

FAQs

What was the main goal of the study on miR-195-5p in gastric cancer?

To explore the potential function and underlying mechanism of miR-195-5p in gastric cancer progression.

How did the researchers test the effect of miR-195-5p on gastric cancer cells?

They measured GC cell function using CCK-8, clone formation, flow cytometry, transwell, and wound healing assays.

What mechanism did the study identify for miR-195-5p’s effects?

miR-195-5p influenced malignant progression through autophagy, binding to LAMP2, with phenotypic effects rescued by LAMP2 overexpression.

Sep 23, 2026

Uploaded by MrHarris58