MYC and NCAPG2 as molecular targets of colorectal cancer and gastric cancer in nursing

Sep 22, 2026

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Colorectal cancer and gastric cancer are major digestive malignancies with unclear biological roles for MYC and NCAPG2. Gene expression datasets GSE49355 and GSE19826 were analyzed to screen differentially expressed genes, build WGCNA co-expression modules, and perform functional enrichment, GSEA, immune infiltration, PPI networking, survival assessment, and comparative toxicogenomics (CTD). The study identified 2 core genes (MYC, NCAPG2) with higher expression in tumor tissues, risk-associated survival patterns, and links to inflammation and immune system disorders.

LanguageEnglish

CategoryResearch & Report

FormatPDF

Pages15

Reading Time38 min

AccessFree to download

Introduction

Materials and Methods (dataset analysis overview)

Results (DEGs, WGCNA, core genes)

Survival and CTD findings

Discussion (nursing relevance)

Abbreviations

® Observational Study MYC and NCAPG2 as molecular targets of colorectal cancer and gastric cancer in nursing Xihua Mi, MDa , Haifeng Shan, MDa , Chunbo Kang, MDa ,* , Jie Zhang, MDa , Shiyang Hou, MDa , Yanfang Gao, MDa , Lingli Hao, MDa , Xiaoli Gao, MDa , Qiang Gao, MDa , Xiaoqian Chi, MDa , Qijun Zhang, MDa | Abstract
Colorectal cancer is a common malignant tumor in intestinal tract, the early symptoms are not obvious. Gastric cancer is a malignant tumor originating from the gastric mucosal epithelium. However, the role of MYC and non-SMC condensin II complex subunit G2 (NCAPG2) in colorectal cancer and gastric cancer remains unclear. The colorectal cancer datasets GSE49355 and gastric cancer datasets GSE19826 were downloaded from gene expression omnibus database. Differentially expressed genes (DEGs) were screened and weighted gene co-expression network analysis (WGCNA) was performed. Functional enrichment analysis, gene set enrichment analysis (GSEA) and immune infiltration analysis was performed. Construction and analysis of protein-protein interactions (PPI) network. Survival analysis and comparative toxicogenomics database (CTD) were performed. A heat map of gene expression was drawn. A total of 751 DEGs were obtained. According to the gene ontology (GO) analysis, in Biological process (BP) analysis, they are mainly enriched in cell differentiation, cartilage development, and skeletal development. In cellular component (CC) analysis, they are mainly enriched in the cytoskeleton of muscle cells and actin filaments. In molecular function (MF) analysis, they are mainly concentrated in Rho GTPase binding, DNA binding, and fibronectin binding. In Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis, they are mainly enriched in the MAPK signaling pathway, apoptosis, and cancer pathways. The soft threshold power for WGCNA analysis was set to 9, resulting in the generation of 40 modules. Ultimately, 2 core genes (MYC and NCAPG2) were identified. The heatmap of core gene expression showed high expression of MYC and NCAPG2 in colorectal cancer tissue samples and low expression in normal tissue samples, while they were core molecules in gastric cancer. Survival analysis indicated that MYC and NCAPG2 were risk factors, showing an upregulation trend with increasing risk scores. CTD analysis revealed associations of MYC and NCAPG2 with colorectal cancer, gastric cancer, inflammation, and immune system diseases. MYC and NCAPG2 are highly expressed in colorectal cancer. The higher the expression of MYC and NCAPG2, the worse the prognosis. MYC and NCAPG2 are core molecules in gastric cancer. | | --- | | Abbreviations: CTD = comparative toxicogenomics database, DEGs = differentially expressed genes, FC = fold change, FDR = false discovery rate, GO = gene ontology, GSEA = gene set enrichment analysis, KEGG = Kyoto encyclopedia of genes and genomes, NCAPG2 = non-SMC condensin II complex subunit G2, PPI = protein-protein interactions, STRING = search tool for the retrieval of interacting genes, WGCNA = weighted gene co-expression network analysis. | | Keywords: colorectal cancer, gastric cancer, molecular targets, MYC, NCAPG2 , nursing | 1. Introduction Colorectal cancer and gastric cancer are 2 common malignant tumors of the digestive system, which differ in terms of pathogenesis, epidemiology, pathological characteristics, harmfulness, and nursing requirements. [1] Colorectal cancer originates within the colon and rectum, typically arising from the cells of the colon or rectal mucosa. [2] Gastric cancer refers to the malignancy formed by the malignant transformation of epithelial cells on the gastric mucosa. [3] Colorectal cancer is common XM and HS contributed equally to this work. The authors have no funding and conflicts of interest to disclose. The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request. The data for this

FAQs

What question does the study address about MYC and NCAPG2?

It evaluates whether MYC and NCAPG2 act as molecular targets in colorectal cancer and gastric cancer, where their roles have remained unclear.

Which datasets and analytical methods were used?

The study used colorectal cancer dataset GSE49355 and gastric cancer dataset GSE19826. It performed DEG screening, WGCNA, functional enrichment, GSEA, immune infiltration, PPI construction, survival analysis, and CTD analysis.

What core genes were identified and what was their expression pattern?

Two core genes—MYC and NCAPG2—were identified. Their heatmaps showed higher expression in colorectal cancer tissues and lower expression in normal tissues, while they were core molecules in gastric cancer.

How did MYC and NCAPG2 relate to prognosis?

Survival analysis indicated MYC and NCAPG2 were risk factors, with an upregulation trend as risk scores increased, corresponding to worse prognosis.

Sep 22, 2026

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