MiR-618 suppresses the proliferation, invasion, and migration of non-small lung cancer via the JAK2/STAT3 axis

Sep 21, 2026

Investigates how the miR-618/JAK2/STAT3 regulatory axis affects non-small cell lung cancer (NSCLC) cells, aiming to identify a precise therapeutic target. Differential gene expression is screened in the GEO database, then supported by bioinformatics. miR-618 levels are quantified by qRT-PCR, while JAK2/STAT3 proteins are assessed by western blotting. miR-618–JAK2 targeting is tested using dual-luciferase assays. Functional assays (MTT, wound-healing, Transwell) evaluate proliferation, migration, and invasion.

LanguageEnglish

CategoryResearch & Report

FormatPDF

Pages9

Reading Time23 min

AccessFree to download

Abstract

Objective

Methods

Results

Conclusion

Introduction

Background on lung cancer

Role of microRNAs in NSCLC

Chen et al. Journal of Cardiothoracic Surgery (2024) 19:679 [https://doi.org/10.1186/s13019-024-03160-5](https://doi.org/10.1186/s13019-024-03160-5) Journal of Cardiothoracic Surgery RESEARCH Open Access MiR-618 suppresses the proliferation, invasion, and migration of non-small lung cancer via the JAK2/STAT3 axis Ziyuan Chen 1, Wei Chen 1, Zhiqi Hong 1,2, Xianqiao Wu 1,2, Tianzheng Fang 1,2, Yufei Sheng 1,2, Shuai Fang 1* and Chengwei Zhou 1,2* Abstract Objective The regulatory role of the miR-618/JAK2/STAT3 axis in non-small cell lung cancer cells (NSCLC) was investigated with the objective of identifying a target for the precise treatment of patients with NSCLC. Methods Initially, differential gene expression was identified in the GEO database, followed by a comprehensive bioinformatics analysis. The tissue and cell levels of miR-618 were assessed using qRT-PCR, while the protein levels of JAK2 and STAT3 were determined through western blotting analysis. The association between miR-618 and JAK2 was scrutinized through bioinformatics analysis and dual-luciferase experiments. To evaluate cell proliferation, migration, and invasion, MTT, wound-healing, and Transwell assays were employed. Results The expression of miR-618 is decreased in NSCLC, and it targets the JAK2/STAT3 pathway to inhibit the proliferation, invasion, and migration of NSCLC. Conclusion Our study demonstrates that a novel miR-618/JAK2/STAT3 signaling axis is involved in suppressing malignancy in NSCLC and provides a promising target for NSCLC therapy. Keywords Non-small cell lung cancer, miRNA, JAK2, miR-618, STAT3 Introduction Lung carcinoma is one of the leading causes of global cancer mortality.Based on the information from GLOBOCAN 2018 data, approximately 2.09 million new cases and 1.76 million deaths are reported each year [1]. Lung cancer ranks among the most prevalent malignant *Correspondence: Shuai Fang[sfang@ncri.org.cn](sfang@ncri.org.cn)[ ](sfang@ncri.org.cn)Chengwei Zhou [nbzhouchengwei@163.com](nbzhouchengwei@163.com) 1Department of Thoracic Surgery, The First Affiliated Hospital of Ningbo University, No. 59, Liuting Road, Haishu District, Ningbo, Zhejiang Province, China 2Health Science Center, Ningbo University, Ningbo, China neoplasms in China. The 2015 data published by the National Cancer Center reveal that the 5-year prevalence rate of lung cancer in China during the period of 2006 to 2011 stood at 130.2 cases per 100,000 individuals.Of these cases, the prevalence rate for men was 84.6 (1/100 000), ranking second for malignant tumors, while the rate for women was 45.6 (1/100 000), ranking fourth for malignant tumors [2]. From a histopathological perspective, the two primary categories of lung carcinoma comprise small cell lung cancer (SCLC) and non-small cell lung cancer (NSCLC), with NSCLC accounting for approximately 85% of all cases based on histological analysis [3]. While the treatment methods for lung cancer have become more diverse, the survival rate of lung cancer patients is still only about 15% with late-stage disease © The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you wi

FAQs

What therapeutic mechanism does the study focus on for NSCLC?

The study focuses on the miR-618/JAK2/STAT3 axis and how it suppresses NSCLC malignant behaviors.

How is miR-618 expression and target signaling evaluated?

miR-618 is measured by qRT-PCR, JAK2 and STAT3 proteins by western blotting, and the miR-618/JAK2 relationship by bioinformatics and dual-luciferase experiments.

Which experiments assess the effects on NSCLC cell behavior?

Cell proliferation, migration, and invasion are evaluated using MTT, wound-healing, and Transwell assays.

Sep 21, 2026

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