miR-30d-5p inhibits proliferation, invasion and migration of breast cancer cells by targeting SERPINE1 and promoting fatty acid β-oxidation

Sep 21, 2026

Breast cancer remains a leading global malignancy with unclear pathogenesis, while fatty acid β-oxidation is strongly linked to tumor biology. SERPINE1-driven down-regulation of β-oxidation supports breast cancer cell proliferation, invasion, and metastasis. The study first compares miR-30d-5p levels in cancerous versus para-carcinoma tissues, then evaluates SERPINE1 and key β-oxidation enzymes (LCAD, MCAD). miR-30d-5p mimics reduce SERPINE1 and increase MCAD/LCAD, suppressing proliferation, invasion, and metastasis.

LanguageEnglish

CategoryResearch & Report

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Pages10

Reading Time25 min

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Abstract

Introduction

Materials and Methods

[www.aging-us.com](www.aging-us.com) AGING 2024, Vol. 16, No. 7 Research Paper miR-30d-5p inhibits proliferation, invasion and migration of breast cancer cells by targeting SERPINE1 and promoting fatty acid β -oxidation Lina Zhang1, Shuguang Ren2, Yang Sang2, Yueyang Hu2, Cong Wang1, Xinrui Wang1, Yuntao Li1 1 Breast Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei, China 2Animal Center, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050000, Hebei, China Correspondence to: Yuntao Li; email: [20151018@stu.hebmu.edu.cn](20151018@stu.hebmu.edu.cn) Keywords: miRNA-30d-5p, SERPINE1, fatty acid beta oxidation, breast cancer proliferation Received: April 13, 2023 Accepted: December 12, 2023 Published: February 22, 2024 Copyright: © 2024 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. ABSTRACT Breast cancer (BC) is among the top three most prevalent cancers across the world, especially in women, and its pathogenesis is still unknown. Fatty acid β-oxidation is highly associated with breast cancer. Serpin family E member 1 (SERPINE1)-induced down-regulation of fatty acid β-oxidation can facilitate BC cell proliferation, invasion, and metastasis. In this paper, the difference of miR-30d-5p expressions in both cancerous tissues and para-carcinoma tissues was first detected. Next, the expressions of SERPINE1, long-chain acyl-CoA dehydrogenase (LCAD) and medium-chain acyl-CoA dehydrogenase (MCAD) in the aforementioned tissues were analyzed. Finally, miR-30d-5p mimics were supplemented to breast cancer cells to observe the miR-30d-5p effect upon breast cancer cells. Via immunofluorescence assay and Western blotting, it was found that cancerous tissues had lower expressions of miR-30d-5p, MCAD and LCAD and a higher expression of SERPINE1 than para-carcinoma tissues. The miR-30d-5p mimic group had a decreased SERPINE1 expression and increased MCAD and LCAD expressions compared with the NC group, thus inhibiting BC cell proliferation, invasion, and metastasis. To sum up, miR-30d-5p blocks the cell proliferation, invasion and metastasis by targeting SERPINE1 and promoting fatty acid β-oxidation. Preclinical studies are further required to establish a fatty acid β-oxidation-targeting therapy for breast cancer. INTRODUCTION Breast cancer (BC) is among the top three most prevalent cancers worldwide, especially in females [1] . It is estimated that there exist approximately 2,088,849 new BC cases all over the world each year, occupying nearly a quarter of cancer cases in women [2] . As the relevant theories and technologies develop, a significant progress has been made in cancer therapy. However, the BC death rate still reaches about 30% . In 2018, the latest data from IARC showed that breast cancer affects 24.2% of women globally, 52.9% of whom are in the developing country [3] . In some developing countries, such as South America, Africa and Asia, breast cancer has already been a leading cause of cancer death, partly due to a lack of access to advanced diagnoses and treatment [4] . The mammary gland is an accessory gland of the skin and is a double-tube alveolar gland. The breast is composed principally of connective tissue and adipose tissue (AT) . AT consists of numerous clusters of fat cells that are divided into lobules via thin and loose connective tissue layers. Based on the structure and function of adipose cells, adipose tissue is classified into yellow (white) AT and brown At, the former of which is the main component of the breast. Fatty acid plays a pivotal part in all stages of cancer [5, 6] . Normally, large amounts of fatty acids are required for rapidly proliferating cells, so that membrane synthesis can be facilitated and phospholipids are form

FAQs

What relationship does SERPINE1 have with fatty acid β-oxidation in breast cancer?

SERPINE1 induction is described as down-regulating fatty acid β-oxidation, which facilitates breast cancer cell proliferation, invasion, and metastasis.

How were miR-30d-5p effects evaluated in breast cancer cells?

miR-30d-5p mimics were supplemented to breast cancer cells, and the downstream changes were assessed using immunofluorescence and Western blotting.

What expression differences were observed between cancerous and para-carcinoma tissues?

Cancerous tissues showed lower miR-30d-5p, MCAD, and LCAD, but higher SERPINE1 compared with para-carcinoma tissues.

Sep 21, 2026

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