miR-223-3p predicts prognosis of hepatitis B virus-related acute-on-chronic liver failure and is involved in hepatocyte injury via HSP90B1

Sep 29, 2026

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Hepatitis B virus-related acute-on-chronic liver failure (HBV-ACLF) is a high-mortality syndrome marked by acute hepatic decompensation over a short period. This study evaluates whether miR-223-3p can predict short-term outcomes and clarifies its mechanistic involvement in hepatocyte injury. miR-223-3p was quantified by qRT-PCR, correlated with severity markers, and analyzed using ROC, Cox, and Kaplan-Meier approaches, with functional experiments in MIHA cells and target validation.

LanguageEnglish

CategoryHealthcare

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Pages12

Reading Time30 min

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Abstract

Introduction

Materials and methods

Results

Conclusions

Xie et al. Hereditas (2026) 163:6 [https://doi.org/10.1186/s41065-025-00610-5](https://doi.org/10.1186/s41065-025-00610-5) Hereditas RESEARCH Open Access miR-223-3p predicts prognosis of hepatitis B virus-related acute-on-chronic liver failure and is involved in hepatocyte injury via HSP90B1 Feiyue Xie 1, Qiuping Ren 1, Jun He 1 and Menghang Wu 1* Abstract Background Hepatitis B virus-related acute-on-chronic liver failure (HBV-ACLF) is a clinical syndrome that presents with acute hepatic decompensation and liver failure in a relatively short time, with a high mortality rate. Objective The aim was to assess the predictive value of miR-223-3p in the short-term prognosis of patients and its potential role in HBV-ACLF, thus providing new ideas for personalized treatment. Materials and methods The level of miR-223-3p was quantified using qRT-PCR. The correlation between miR- 223-3p level and indicators associated with the severity of HBV-ACLF (TBil, INR, and MELD score) was assessed using Spearman’s method. The prognostic value of miR-223-3p in HBV-ACLF was assessed using the ROC curve, Cox regression analysis, and Kaplan-Meier curve. To detect the proliferation and apoptosis of MIHA cells, CCK-8 assay and flow cytometry were employed. Bioinformatics methods were conducted to identify the downstream targets of miR- 223-3p. The regulation between miR-223-3p and HSP90B1 was validated through Dual-luciferase reporter gene assay. Results In patients with HBV-ACLF, miR-223-3p expression was reduced and negatively correlated with TBil, INR, and MELD score. Low expression of miR-223-3p predicted adverse prognosis for patients. Furthermore, MELD score and miR-223-3p were identified as independent prognostic factors in patients with HBV-ACLF. In H2O2 orTNF-α–induced MIHA cells, miR-223-3p facilitated cellular proliferation and suppressed apoptosis. The role of miR-223-3p in hepatocyte injury was mediated by HSP90B1 . Conclusions Serum miR-223-3p expression was predictive for short-term survival in patients with HBV-ACLF, and miR- 223-3p attenuated hepatocellular injury in vitro by modulating HSP90B1 . Keywords MiR-223-3p, HBV, Liver failure, Prognosis *Correspondence: Menghang Wu [wumengh1126@163.com](wumengh1126@163.com) 1Department of Liver Surgery, West China Hospital of Sichuan University, No. 37, Guoxue Lane, Wuhou District, Chengdu 610044, China © The Author(s) 2025. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit [http://creativecommons.org/l](http://creativecommons.org/l)icenses/by/4.0/. The Creative Commons Public Domain Dedication waiver ([http://creativecommons.org/publicdomain/zero/1.0/](http://creativecommons.org/publicdomain/zero/1.0/)) applies to the data made available in this article, unless otherwise stated in a credit line to the data. Xie et al. Hereditas (2026) 163:6 Introduction Acute-on-chronic liver failure (ACLF) is a syndrome characterized by the development of coagulation dysfunction and acute jaundice in individuals with chronic liver disease [1]. It can be precipitated by various triggers, including hemorrhage, infection, and other external stimuli [2]. Hepatitis B virus (HBV)-ACLF has the highest prevalence in China and is characte

FAQs

What clinical question does the study address about HBV-ACLF?

It assesses whether miR-223-3p has predictive value for short-term prognosis in HBV-ACLF patients and explores its role in hepatocyte injury.

How was miR-223-3p level measured and linked to disease severity?

miR-223-3p was quantified using qRT-PCR, and its correlation with severity indicators (TBil, INR, and MELD score) was evaluated using Spearman’s method.

What mechanistic pathway involving HSP90B1 was proposed?

The study reports that miR-223-3p influences hepatocyte injury mediated by HSP90B1, supported by dual-luciferase reporter validation and cell experiments showing effects on proliferation and apoptosis.

Sep 29, 2026

Uploaded by Rizky