MicroRNAs miR-16 and miR-519 control meningioma cell proliferation via overlapping transcriptomic programs shared with the RNA-binding protein HuR

Aug 26, 2026

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MicroRNAs miR-16 and miR-519 are investigated as regulators of meningioma growth and tumorigenesis, focusing on how they coordinate gene-expression programs. The study quantifies miR-16/miR-519 in human intracranial grade 1–2 meningiomas and compares tumor levels with healthy meningeal tissues. Functional assays in benign and aggressive meningioma cell lines show that restoring either microRNA reduces cell growth. Transcriptomic analyses identify downregulated pathways linked to cell-cycle control and a signature enriched in HuR targets, supporting a tumor-suppressive mechanism through HuR modulation.

LanguageEnglish

CategoryResearch & Report

FormatPDF

Pages15

Reading Time38 min

AccessFree to download

Introduction

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TYPE Original Research PUBLISHED 02 August 2023 DOI 10.3389/fonc.2023.1158773 OPEN ACCESS EDITED BY Yuriy Gusev, Georgetown University, United States REVIEWED BY Sabrina Battista, National Research Council (CNR), Italy Sharon K. Michelhaugh, Virginia Tech, United States *CORRESPONDENCE Se´ bastien Hergalant [sebastien.hergalant@univ-lorraine.fr](sebastien.hergalant@univ-lorraine.fr)[ ](sebastien.hergalant@univ-lorraine.fr)Guillaume Gauchotte [g.gauchotte@chru-nancy.fr](g.gauchotte@chru-nancy.fr) †These authors have contributed equally to this work and share last authorship RECEIVED 04 February 2023 ACCEPTED 14 July 2023 PUBLISHED 02 August 2023 CITATION Hergalant S, Casse J-M, Oussalah A, Houlgatte R, Helle D, Rech F, Vallar L, Gue´ ant J-L, Vignaud J-M, Battaglia-Hsu S-F and Gauchotte G (2023) MicroRNAs miR-16 and miR-519 control meningioma cell proliferation via overlapping transcriptomic programs shared with the RNA-binding protein HuR. Front. Oncol. 13:1158773 . doi: 10.3389/fonc.2023.1158773 COPYRIGHT © 2023 Hergalant, Casse, Oussalah, Houlgatte, Helle, Rech, Vallar, Gue´ant, Vignaud, Battaglia-Hsu and Gauchotte. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. MicroRNAs miR-16 and miR-519 control meningioma cell proliferation via overlapping transcriptomic programs shared with the RNA-binding protein HuR S´ebastien Hergalant 1*, Jean-Matthieu Casse 1, Abderrahim Oussalah 1,2,3, R´emi Houlgatte 1, D´eborah Helle 1, Fabien Rech 4,5, Laurent Vallar6, Jean-Louis Gu´eant 1,2,3, Jean-Michel Vignaud1,7,8, Shyue-Fang Battaglia-Hsu 2,3,5† and Guillaume Gauchotte 1,7,8*† 1 INSERM, U1256, NGERE – Nutrition, Genetics, and Environmental Risk Exposure, Faculty of Medicine of Nancy, University of Lorraine, Vandoeuvre-lès-Nancy, France, 2 Department of Molecular Medicine and Personalized Therapeutics, University Hospital of Nancy (CHRU), Vandoeuvre-lès-Nancy, France, 3 Department of Biochemistry, Molecular Biology, Nutrition, and Metabolism, University Hospital of Nancy (CHRU), Vandoeuvre-lès-Nancy, France, 4 Department of Neurosurgery, University Hospital of Nancy (CHRU), Nancy, France, 5CNRS, UMR7039, CRAN-Centre de Recherche en Automatique de Nancy, Universite´ de Lorraine, Vandoeuvre-lès-Nancy, France, 6Genomics and Proteomics, Department of Oncology, Luxembourg Institute of Health, Luxembourg, Luxembourg, 7 Department of Biopathology Institut De Cance´ rologie de Lorraine (CHRU-ICL), University Hospital of Nancy (CHRU), Nancy, France, 8Centre de Ressources Biologiques BB-0033-00035, University Hospital of Nancy (CHRU), Nancy, France Introduction: Meningiomas are the most common type of primary central nervous system tumors. In about 80% cases, these tumors are benign and grow very slowly, but the remainder 20% can unlock higher proliferation ratesand become malignant. In this study we examined two miRs, miR-16 and miR- 519, and evaluated their role in tumorigenesis and cell growth in human meningioma. Methods: A cohort of 60 intracranial grade 1 and grade 2 human meningioma plus 20 healthy meningeal tissues was used to quantify miR-16 and miR-519 expressions. Cell growth and dose-response assays were performed in two human men ingio ma cell lines, Ben-Men-1 (benign) and IOMM-Lee (aggressive) . Transcriptomes of IOMM-lee cells were measured after both miRmimics transfection, followed by integrative bioinformatics to expand on available data. Results: In tumoral tissues, we detected decreased levels of miR-16 and miR-519 when compared with arachnoid cells of healthy patients (miR-16: P

FAQs

What microRNAs are studied in meningioma cells?

The study focuses on miR-16 and miR-519 as regulators of meningioma cell proliferation and tumorigenesis.

How are miR-16 and miR-519 expression levels different in tumors vs healthy tissue?

Tumoral tissues show decreased levels of miR-16 and miR-519 compared with arachnoid cells from healthy patients.

What mechanism links miR-16/miR-519 to reduced meningioma growth?

Downregulated genes in aggressive cells include ELAVL1/HuR-linked effects, and the dysregulated transcriptomic signature is highly enriched in HuR target genes, indicating HuR-mediated inhibition.

Aug 26, 2026

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