MicroRNA Expression Profile in Bone Marrow and Lymph Nodes in B-Cell Lymphomas

Q: What is the study aim regarding miRNA expression in B-cell lymphomas?

To analyze unique miRNA expression profiles across different B-cell lymphoma types in lymph nodes and bone marrow.

Q: Which methods were used to measure miRNA expression levels?

Expression levels were measured using the real-time RT-PCR method.

Q: What key finding links miRNA profiles to lymphoma type and tissue location?

miRNA expression profiles differ between bone marrow and lymph nodes depending on the B-cell lymphoma type, likely influenced by the tumor microenvironment.

Sep 24, 2026

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Hodgkin’s lymphomas and most non-Hodgkin’s lymphomas arise from distinct stages of B-cell differentiation, and microRNA (miRNA) expression profiles evolve throughout lymphopoiesis. This study analyzes unique miRNA expression signatures across B-cell lymphoma types in lymph nodes and bone marrow. Real-time RT-PCR quantifies miRNAs including members of the miR-183-96-182 cluster and miRNA-18a, -20a, -96, -182, -183, -26b, -34a, -148b, -9, -150, -451a, -23b, -141, and -128. Results show differential miRNA patterns between bone marrow and lymph nodes by lymphoma subtype, reflecting tumor microenvironment effects and supporting diagnostic discrimination.

LanguageEnglish

CategoryResearch & Report

FormatPDF

Pages13

Reading Time33 min

AccessFree to download

Abstract

Introduction

Background: lymphoma heterogeneity and B-cell differentiation

miRNA regulation in lymphopoiesis and hematopoiesis

Prior findings on miRNAs in B-cell lymphoma subtypes

Article MicroRNA Expression Proﬁle in Bone Marrow and Lymph Nodes in B-Cell Lymphomas Yuliya A. Veryaskina 1,2, *, Sergei E. Titov 1,3, Igor B. Kovynev 4, Tatiana I. Pospelova 4, Sofya S. Fyodorova 4, Yana Yu. Shebunyaeva 4, Dina V. Sumenkova 4 and Igor F. Zhimulev 1 Citation: Veryaskina, Y.A.; Titov, S.E.; Kovynev, I.B.; Pospelova, T.I.; Fyodorova, S.S.; Shebunyaeva, Y.Y.; Sumenkova, D.V.; Zhimulev, I.F. MicroRNA Expression Proﬁle in Bone Marrow and Lymph Nodes in B-Cell Lymphomas. Int. J. Mol. Sci. 2023, 24, 15082. [https://doi.org/](https://doi.org/)[ ](https://doi.org/)[10.3390/ijms242015082](10.3390/ijms242015082) Academic Editor: Andrey Turchinovich Received: 22 August 2023 Revised: 26 September 2023 Accepted: 27 September 2023 Published: 11 October 2023 Copyright: © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ([https://](https://)[ ](https://)[creativecommons.org/licenses/by/](creativecommons.org/licenses/by/)[ ](creativecommons.org/licenses/by/)[4.0/](4.0/)) . 1 Department of the Structure and Function of Chromosomes, Laboratory of Molecular Genetics, Institute of Molecular and Cellular Biology, SB RAS, 630090 Novosibirsk, Russia; [titovse78@gmail.com](titovse78@gmail.com) (S.E.T.); [zhimulev@mcb.nsc.ru](zhimulev@mcb.nsc.ru) (I.F.Z.) 2 Laboratory of Gene Engineering, Institute of Cytology and Genetics, SB RAS, 630090 Novosibirsk, Russia 3 AO Vector-Best, 630117 Novosibirsk, Russia 4 Department of Therapy, Hematology and Transfusiology, Novosibirsk State Medical University, 630091 Novosibirsk, Russia; [kovin_gem@mail.ru](kovin_gem@mail.ru) (I.B.K.); [depart04@mail.ru](depart04@mail.ru) (T.I.P.); [soffka.tv@mail.ru](soffka.tv@mail.ru) (S.S.F.); [jana.shebuniaeva@yandex.ru](jana.shebuniaeva@yandex.ru) (Y.Y.S.); [dinasumenkova@mail.ru](dinasumenkova@mail.ru) (D.V.S.) * [Correspondence: microrna@inbox.ru](Correspondence: microrna@inbox.ru) Abstract: Hodgkin's lymphomas (HL) and the majority of non-Hodgkin's lymphomas (NHL) derive from different stages of B-cell differentiation. MicroRNA (miRNA) expression proﬁles change during lymphopoiesis. Thus, miRNA expression analysis can be used as a reliable diagnostic tool to differentiate tumors. In addition, the identiﬁcation of miRNA's role in lymphopoiesis impairment is an important fundamental task. The aim of this study was to analyze unique miRNA expression proﬁles in different types of B-cell lymphomas. We analyzed the expression levels of miRNA-18a,-20a, -96, -182, -183, -26b, -34a, -148b, -9, -150, -451a, -23b, -141, and-128 in lymph nodes (LNs) in the following cancer samples: HL (n = 41), diffuse large B-cell lymphoma (DLBCL) (n = 51), mantle cell lymphoma (MCL) (n = 15), follicular lymphoma (FL) (n = 12), and lymphadenopathy (LA) (n = 37), as well as bone marrow (BM) samples: HL (n = 11), DLBCL (n = 42), MCL (n = 14), FL (n = 16), and non-cancerous blood diseases (NCBD) (n = 43) . The real-time RT-PCR method was used for analysis. An increase in BM expression levels of miRNA-26b, -150, and-141 in MCL (p < 0.01) and a decrease in BM levels of the miR-183-96-182 cluster and miRNA-451a in DLBCL (p < 0.01) were observed in comparison to NCBD. We also obtained data on increased LN levels of the miR-183-96-182 cluster in MCL (p < 0.01) and miRNA-18a, miRNA-96, and miRNA-9 in FL (p < 0.01), as well as decreased LN expression of miRNA-150 in DLBCL (p < 0.01), and miRNA-182, miRNA-150, and miRNA-128 in HL (p < 0.01) . We showed that miRNA expression proﬁle differs between BM and LNs depending on the type of B-cell lymphoma. This can be due to the effect of the tumor microenvironment. Keywords: microRNA; Hodgkin's lymphomas; non-Hodgkin's lymphomas; B-cell lymphoma; bone marrow; lymph nodes 1. Introduction According to the classiﬁcation of hematolymphoid tumors, the term lymphoma covers a hetero

FAQs

What is the study aim regarding miRNA expression in B-cell lymphomas?

To analyze unique miRNA expression profiles across different B-cell lymphoma types in lymph nodes and bone marrow.

Which methods were used to measure miRNA expression levels?

Expression levels were measured using the real-time RT-PCR method.

What key finding links miRNA profiles to lymphoma type and tissue location?

miRNA expression profiles differ between bone marrow and lymph nodes depending on the B-cell lymphoma type, likely influenced by the tumor microenvironment.

Sep 24, 2026

Upload by McGucket