Long noncoding RNA KCNMA1-AS2 regulates the function of colorectal cancer cells and sponges miR-1227-5p

Sep 21, 2026

Uploaded by Noah

Long noncoding RNA (lncRNA) expression changes can significantly influence colorectal cancer (CRC) progression, yet many CRC-related lncRNA functions remain unclear. This study identified KCNMA1-AS2 as a down-regulated lncRNA in CRC tissues and cell lines and examined how its overexpression affects tumor cell behavior. Using sequencing, expression validation, cell functional assays, dual-luciferase reporter testing, and qPCR, the work links KCNMA1-AS2 to miR-1227-5p sponging and highlights potential CRC biomarker and therapeutic relevance.

LanguageEnglish

CategoryResearch & Report

FormatPDF

Pages12

Reading Time30 min

AccessFree to download

Introduction

Background and significance

Methods

Sequencing and expression validation

Functional assays

miR-1227-5p interaction testing

Bioinformatics target prediction

Results

KCNMA1-AS2 expression pattern

Effects of KCNMA1-AS2 overexpression

Evidence for KCNMA1-AS2/miR-1227-5p interaction

Predicted downstream links

Conclusions

Miao et al. BMC Cancer (2024) 24:857 BMC Cancer [https://doi.org/10.1186/s12885-024-12608-9](https://doi.org/10.1186/s12885-024-12608-9) RESEARCH Open Access Long noncoding RNA KCNMA1-AS2 regulates the function of colorectal cancer cells and sponges miR-1227-5p Xinzhi Miao 1,2,3, Fang Wang2,3,4, Muhammad Amir Yunus2, Ida Shazrina Ismail 2* and Tianyun Wang3* Abstract Background Many long noncoding RNAs (lncRNAs) with altered expression significantly influence colorectal cancer (CRC) progression and behavior. The functions of many lncRNAs in CRC are not clear yet. This study aimed to discover novel l ncRNA entities and comprehensively examine and validate their roles and underlying molecular mechanisms in CRC. Methods Tissue samples, both tumourous and non-tumourous, from three CRC patients were submitted for sequencing. Following expression validation in samples from ten patients and four CRC cell lines. The lncRNAKCNMA1-AS2 was synthesized by In-vitro transcription RNA synthesis and the lncRNA was directly transfected into CRC cell lines to overexpress. Functional assays including MTT proliferation assay, Annexin-V/propidium iodide apoptosis assay, wound healing migration assay and cell cycle assays were performed to evaluate the effect of overexpression of KCNMA1-AS2 . Furthermore, the binding of KCNMA1-AS2 to miR-1227-5p was confirmed using dual luciferase reporter assays and qPCR analyses. Subsequent bioinformatics analyses identified 58 potential downstream targets of miR-1227-5p across three databases. Results In this study, we identified the lncRNA KCNMA1-AS2, the expression of which was down-regulated consistently in cancer tissues and CRC cell lines compared to non-cancerous tissues. The overexpression of lncRNAKCNMA1-AS2 led to significant reduction in CRC cell proliferation and migration, increase in cell apoptosis, and more cells arrested in S phase. Additionally, the interaction between KCNMA1-AS2 and miR-1227-5p was confirmed through dual luciferase reporter assay and qPCR analysis. It is also putatively predicted that MTHFR and ST8SIA2 maybe linked to CRC based on bioinformatics analyses. Conclusions LncRNA KCNMA1-AS2 exhibited distinct gene expression patterns in both CRC tissue and cell lines, impacting various cellular functions while also acting as a sponge for miR-1227-5p.The findings spotlight lncRNAKCNMA1-AS2 as a potential marker for diagnosis and treatment of CRC. Keywords KCNMA1-AS2, Biomarker, Colorectal cancer, miR-1227-5p, Long noncoding RNA *Correspondence: Ida Shazrina Ismail[idashazrina@usm. my](idashazrina@usm. my)[ ](idashazrina@usm. my)Tianyun Wang [wty@xxmu.edu.cn](wty@xxmu.edu.cn) 1The School of Medical Humanities, Xinxiang Medical University, Xinxiang, Henan province 453003, China 2Department of Biomedical Sciences, Advanced Medical and Dental Institute, Universiti Sains Malaysia, Bertam, Kepala Batas, Penang 13200, Malaysia 3Henan International Joint Laboratory of Recombinant Pharmaceutical Protein Expression System, Xinxiang Medical University, Xinxiang, Henan province 453003, China 4Department of Biochemistry and Molecular Biology, Xinxiang Medical University, Xinxiang, Henan province 453003, China © The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the

FAQs

What is the main focus of this study?

The study investigates how the lncRNA KCNMA1-AS2 regulates colorectal cancer cell function and whether it acts by sponging miR-1227-5p.

How was the role of KCNMA1-AS2 tested experimentally?

Researchers used sequencing and expression validation, then synthesized and transfected KCNMA1-AS2 into CRC cell lines. They evaluated proliferation, apoptosis, migration, and cell-cycle changes with assays such as MTT, Annexin V/PI, wound healing, and cell-cycle analysis.

How did the study confirm interaction between KCNMA1-AS2 and miR-1227-5p?

The binding was supported using dual-luciferase reporter assays and qPCR analyses, showing that KCNMA1-AS2 interacts with miR-1227-5p.

Sep 21, 2026

Uploaded by Noah