LINC02466 promotes the progression of hepatocellular carcinoma through the mTOR pathway

Sep 21, 2026

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Objective focuses on LINC02466, a long non-coding RNA newly associated with adverse outcomes in primary liver cancer and its escalation. Methods measure LINC02466 expression in malignant and adjacent normal tissues by RT-qPCR and Western blot, and evaluate proliferation, colony formation, and cell-cycle effects with CCK8, flow cytometry, and survival analysis. Results show that LINC02466 suppression reduces stemness, while overexpression accelerates tumor growth. Conclusion supports an mTOR-dependent mechanism, suggesting LINC02466 as a potential therapeutic target.

LanguageEnglish

CategoryHealthcare

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Pages15

Reading Time38 min

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Research LINC02466 promotes the progression of hepatocellular carcinoma through the mTOR pathway Shiqian Liu1 · Zhipeng Quan1 · Jiaming Liang1 · Fuqiang Wang2 · Hao Yan1 · Zhenran Wang2 · Bo Tang1 · Xuebin Qin2 Received: 25 February 2024 / Accepted: 26 April 2024 © The Author(s) 2024 OPEN Abstract Objective Long non-coding RNAs (lncRNAs) LINC02466 is an lncRNA newly linked to the adverse outcomes in primary liver cancer patients, and its crucial involvement in the disease’s escalation. Decoding the specific role of LINC02466 in HCC progression is of great significance to provide a potential therapeutic target for HCC. Methods RT-qPCR and Western Blot techniques was used to analyze the expression levels of LINC02466 in both malignant and surrounding healthy liver tissues. CCK8 assays and colony formation experiments indicates the LINC02466’s effect on the proliferation rates of liver cancer cells. Flow cytometry was pivotal in revealing its significant influence on the cell cycle of these cells. Kaplan–Meier survival analysis with log-rank tests were employed. Results The suppression of LINC02466 markedly reduces the stemness attributes of liver cancer cells, indicating a potential therapeutic target. LINC02466 overexpression significantly increased tumor growth rates and final volumes. Further research indicated that LINC02466 significantly influences liver cancer progression through regulating the mTOR signaling pathway. Conclusion LINC02466 regulating cell proliferation, the cell cycle, and stemness characteristics via the mTOR pathway, suggesting LINC02466 as a potential therapeutic target for primary liver cancer. Keywords LINC02466 · HCC · mTOR · Proliferation · Stem cell 1 Introduction Hepatocellular Carcinoma (HCC) ranks prominently among global digestive tract malignancies, ranking as the fifth most prevalent form of cancer globally and stands as the third most common cause of death from cancer [1] . HCC represents about 90% of all liver cancer diagnoses [2]. Notably, in China, HCC is the primary cause of death among individuals under 65 years, with incidence and mortality rates significantly higher in males than in females [3, 4]. In 2020, China reported an Shiqian Liu, Zhipeng Quan, and Jiaming Liang have contributed equally to this work. Supplementary Information The online version contains supplementary material available at [https://doi.org/10.1007/s12672-024-](https://doi.org/10.1007/s12672-024-)[ ](https://doi.org/10.1007/s12672-024-)[00999-x](00999-x.)[.](00999-x.) * Zhenran Wang, [w07ll07@163.com](w07ll07@163.com); * Bo Tang, [dr_sntangbo@163.com](dr_sntangbo@163.com); * Xuebin Qin, [qxuebin@163.com |](qxuebin@163.com |1Department)[1](qxuebin@163.com |1Department)[Department](qxuebin@163.com |1Department)[ ](qxuebin@163.com |1Department)[of Hepatobiliary Surgery](of Hepatobiliary Surgery), [The](The) First Affiliated Hospital of Guangxi Medical University, No. 6 Shuangyong Road, Nanning 530021, Guangxi, People’s Republic of China. 2Department of Gastrointestinal Surgery and Hepatobiliary Surgery and Department of Critical Care Medicine, Guilin Medical University, Affiliated Hospital, Guilin 541001, Guangxi, People’s Republic of China. Discover Oncology (2024) 15:623 | [https://doi.org/10.1007/s12672-024-00999-x](https://doi.org/10.1007/s12672-024-00999-x) estimated 410,038 new cases of HCC, accounting for 45.27% of the global incidence, and 391,152 deaths, representing 47.12% of global mortality from the disease [5] . In the field of genomics, it’s established that about 75% of the human genome can be transcribed into RNA, yet only approximately 3% of the genome encodes proteins. The remaining transcripts are non-coding RNAs (ncRNAs), including long non-coding RNAs (lncRNAs) [6–9] . lncRNAs participate in gene expression regulation through complex molecular mechanisms, such as modulating the interaction between transcription factors and promoters, serving as scaffolds fo

FAQs

What is the main objective of the study on LINC02466 in hepatocellular carcinoma?

To determine the role of the lncRNA LINC02466 in HCC progression and assess its potential as a therapeutic target.

How was LINC02466 expression and its effects on cancer cells evaluated?

RT-qPCR and Western blot measured LINC02466 expression, while CCK8 and colony formation assessed proliferation and flow cytometry analyzed effects on the cell cycle.

What did the study find when LINC02466 was suppressed or overexpressed?

Suppression reduced liver cancer cell stemness attributes, whereas overexpression increased tumor growth rates and final tumor volumes.

Sep 21, 2026

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