International Subject Test - Biology Practice Test - Part 1 - 45 Minutes - 38 Questions

Sep 30, 2026

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AIST International Subject Test Biology Practice Test Part 1 provides timed multiple-choice questions with answer options and guidance on pacing, including a 45-minute, 38-question format. The test covers core biology topics such as cell differences, muscle tissue structure and control, bond energy, ion transport across membranes, carbohydrate functions, and experimental design using a light-dependent lettuce seed germination passage. It includes questions on selecting variables, interpreting results, and applying scientific procedures.

LanguageEnglish

CategoryExam

FormatPDF

Pages28

Reading Time1 h 11 min

AccessFree to download

Part 1

Instructions

Biology questions

International Subject Tests International Subject Test—Biology Practice Test The ACT® International Subject Test—Biology Practice Test is an official AIST practice test. The full-length Biology practice test consists of items drawn from the International Subject Test Biology formative assessment pool and adheres to the AIST Biology Test Specifications. This pdf file includes Biology Practice Test questions and answer keys. Taking the AIST Official full-length practice test is the best way to prepare for the two sections of the AIST Biology test. Biology Part 1 45 Minutes—38 Questions For each question, choose the best answer. Your score will be based only on the number of questions you answer correctly during the time allowed. You will not be penalized for guessing. It is to your advantage to answer every question even if you must guess. If you finish before time ends, you should use the time remaining to reconsider questions you are uncertain about. © 2025 by ACT Education Corp. All rights reserved. i No part of the AIST Biology practice test can be copied, reproduced, sold, scanned, emailed, or otherwise transferred. 1. Which of the following is a major difference between prokaryotic and eukaryotic cells? A. Only prokaryotes have nuclei. B. Only prokaryotes have photosynthetic pigments. C. Only eukaryotes have methods of locomotion. D. Only eukaryotes have membrane-bound organelles. 2. Which of the following best describes the appearance and movement of cardiac muscle tissue? A. Striated with voluntary movement B. Striated with involuntary movement C. Nonstriated with voluntary movement D. Nonstriated with involuntary movement Please refer to the following passage to answer questions 3–5. Lettuce seeds require light for germination. A biologist designed an experiment to study the effects of different wavelengths (colors) of light on the germination of a certain variety of light-sensitive lettuce seed. She placed filter paper in the bottom of each of 7 petri plates and moistened the paper with water. Then, she evenly distributed 100 lettuce seeds of the same variety over the filter paper in each plate and covered the plates with glass lids. Each plate received a different light color treatment—white, red, yellow, green, blue—or no light (darkness) for the same amount of time. The relative intensity of the light, the temperature (23°C), and the relative humidity (60%) remained constant. The biologist recorded her data in this table. | Seed Germination | | | --- | --- | | Light color
treatment | \# of seeds
germinated | | White | 92 | | Red | 91 | | Yellow | 21 | | Green | 25 | | Blue | 29 | | No light | 0 | © 2025 by ACT Education Corp. All rights reserved. 1 No part of the AIST Biology practice test can be copied, reproduced, sold, scanned, emailed, or otherwise transferred. 3. What is the independent variable in this experiment? A. Humidity B. Temperature C. Color of light D. Number of seeds germinated 4. Following proper scientific procedures, is it appropriate to include the white-light and no-light treatments, and why? A. Yes; white light is a control, and no light is a control. B. Yes; white light contains all colors of light, but darkness contains only black light. C. No; the experiment is about light so darkness is an unnecessary treatment. D. No; white light contains all colors, making it difficult to determine which color hasan effect. 5. Which of the following statements is an appropriate interpretation of the data for this experiment? A. Blue light promotes germination more than red light. B. Green light stops production of a chemical that inhibits germination. C. Red light promotes germination more than yellow light. D. Lettuce seeds germinate at equal rates in all colors of light. © 2025 by ACT Education Corp. All rights reserved. 2 No part of the AIST Biology practice test can be copied, reproduced, sold, scanned, emai

FAQs

How should test-takers approach guessing on this practice test?

No penalty is given for guessing, so answering every question is to your advantage. If time remains, revisit uncertain questions.

What experiment question asks about the independent variable in the lettuce germination study?

Question 3 asks what the independent variable is, with options including humidity, temperature, color of light, and the number of seeds germinated.

Which question focuses on interpreting the data from the lettuce seed germination table?

Question 5 asks which statement appropriately interprets the data, offering choices about how blue, green, red, or all light colors affect germination.

Sep 30, 2026

Upload by Rhys