Importance of LINC00852/miR-145-5p in breast cancer - A bioinformatics and experimental study

Q: What is the main objective of the study on LINC00852/miR-145-5p in breast cancer?

To examine the importance of the lncRNA LINC00852 in breast cancer pathogenesis and its connection with the miR-145-5p signature.

Q: How were the findings validated in this study?

The study used multiple online bioinformatics tools, then validated the results in 50 pairs of breast cancer tissues and adjacent non-cancerous tissues, and re-evaluated prior miR-145-5p signature data.

Q: What diagnostic value did LINC00852 show between cancerous and adjacent tissues?

LINC00852 separated breast cancer tissues from adjacent non-malignant tissues with an AUC of 0.7218 and a P value < 0.001.

Sep 21, 2026

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Purpose examines the role of lncRNA LINC00852 in breast cancer pathogenesis. Materials and methods integrate multiple online bioinformatics tools with validation in 50 pairs of breast cancer tissues and adjacent non-cancerous samples, and re-evaluate a previously reported miR-145-5p signature dataset. Results show down-regulation of LINC00852 in silico, while expression assays demonstrate significant up-regulation in Iranian patient tumors; miR-145-5p is under-expressed. LINC00852 discriminates tumor versus adjacent tissue (AUC 0.7218, P < 0.001). Conclusion supports LINC00852/miR-145-5p as a potential contributor to disease development.

LanguageEnglish

CategoryResearch & Report

FormatPDF

Pages13

Reading Time33 min

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Abstract

Purpose and aim

Materials and methods

Results and conclusions

Introduction

Analysis Importance of LINC00852/miR‑145‑5p in breast cancer: a bioinformatics and experimental study Abbas Shakoori1,2 · Asghar Hosseinzadeh3 · Nahid Nafisi4 · Ramesh Omranipour5,6 · Leyla Sahebi7 · Nazanin Hosseinkhan8 · Mohsen Ahmadi9 · Soudeh Ghafouri‑Fard9 · Maryam Abtin1,9 Received: 7 September 2024 / Accepted: 7 November 2024 © The Author(s) 2024 OPEN Abstract Purpose We aimed to examine the importance of an lncRNA, namely LINC00852, in the pathogenesis of breast cancer. Materials and methods In the current study, we used several online tools to examine the importance of LINC00852 in breast cancer. Then, we examined these findings in 50 pairs of breast cancer tissues and adjacent non-cancerous ones. We also re-evaluated the data of miR-145-5p signature from our recent study. Results While in silico tools revealed down-regulation of LINC00852 in breast cancer samples, expression assays showed significant up-regulation of this lncRNAs in breast cancer samples compared with matching control samples from Iranian patients. miR-145-5p was under-expressed in breast cancer samples compared with non-cancerous samples. LINC00852 could separate breast cancer tissues from adjacent non-malignant tissues with an AUC value of 0.7218 (P value < 0.001) . Conclusion The current study potentiates LINC00852/miR-145-5p axis as a possible contributor to the pathogenesis of breast cancer. Keywords LINC00852 · miR-145-5p · Breast cancer 1 Introduction Breast cancer is the most frequent malignancy that impends female health with an approximate rate of 1.5 million newly diagnosed cases each year [1, 2]. The most effective way to overcome the cancer is prompt detection and precision treatment, which relies on identification of the pathogenesis of this malignancy. As the tumorigenic mechanisms of breast cancer are complex, discovery of novel biomolecular markers and therapeutic targets is crucial for lengthening patients’survival and refining their quality of life [3] . Long noncoding RNAs (lncRNAs) are a great category of transcripts which includes those with transcript length of over 200 nt with restricted protein coding potential [2, 4]. Recently, lncRNAs have attracted more attention to their emerging * Soudeh Ghafouri-Fard, [s.ghafourifard@sbmu.ac.ir](s.ghafourifard@sbmu.ac.ir); * Maryam Abtin, [m.abtin.genetics@gmail.com |](m.abtin.genetics@gmail.com |1Department of Medical Genetics)[1](m.abtin.genetics@gmail.com |1Department of Medical Genetics)[Department of Medical Genetics](m.abtin.genetics@gmail.com |1Department of Medical Genetics), School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. 2Department of Medical Genetics, Cancer Institute of Iran, Imam Khomeini Hospital Complex, Tehran University of Medical Sciences, Dr. Qarib St., Keshavarz Blvd, Tehran, Iran. 3Department of Biology Education, Farhangian University, Tehran, Iran. 4Surgery Department, Rasoul Akram Hospital, Clinical Research Development Center (RCRDC), Iran University of Medical Sciences, Tehran, Iran. 5Breast Disease Research Center (BDRC), Tehran University of Medical Sciences, Tehran, Iran. 6Department of Surgical Oncology, Cancer Institute, Tehran University of Medical Sciences, Tehran, Iran. 7Maternal, Fetaland Neonatal Research Center, Family Health Research Institute, Tehran University of Medical Sciences, Tehran, Iran. 8Endocrine Research Center, Institute of Endocrinology and Metabolism, Iran University of Medical Sciences (IUMS), Tehran, Iran. 9Department of Medical Genetics, Shahid Beheshti University of Medical Sciences, Tehran, Iran. Discover Oncology (2024) 15:672 | [https://doi.org/10.1007/s12672-024-01553-5](https://doi.org/10.1007/s12672-024-01553-5) role as tumor biomarkers for early discovery, diagnosis, prognostic evaluations, and extrapolation of therapeutic response because of their functions in different cellular processes such as proliferation, apoptotic pathways, metastases, metabolism, chemoresist

FAQs

What is the main objective of the study on LINC00852/miR-145-5p in breast cancer?

To examine the importance of the lncRNA LINC00852 in breast cancer pathogenesis and its connection with the miR-145-5p signature.

How were the findings validated in this study?

The study used multiple online bioinformatics tools, then validated the results in 50 pairs of breast cancer tissues and adjacent non-cancerous tissues, and re-evaluated prior miR-145-5p signature data.

What diagnostic value did LINC00852 show between cancerous and adjacent tissues?

LINC00852 separated breast cancer tissues from adjacent non-malignant tissues with an AUC of 0.7218 and a P value < 0.001.

Sep 21, 2026

Uploaded by Quinn