Impacts of Matrix Metalloproteinase-2 Promoter Genotypes on Breast Cancer Risk

Sep 21, 2026

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Matrix metalloproteinase-2 (MMP-2) is linked to breast cancer pathogenesis, yet evidence on how MMP-2 promoter genotypes influence breast cancer risk remains limited. This study assessed associations between promoter polymorphisms rs243865 and rs2285053 and breast cancer risk using PCR-based RFLP in 1,232 cases and 1,232 controls. Control genotypes followed Hardy-Weinberg equilibrium, and genotype or allele distributions showed no statistically significant differences or interactions. Variant genotypes suggested a minimal, non-significant protective trend for breast cancer and TNBC.

LanguageEnglish

CategoryHealthcare

FormatPDF

Pages9

Reading Time23 min

AccessFree to download

Abstract

Background/Aim

Materials and Methods

Results

Conclusion

Keywords

Introduction

Breast cancer burden and TNBC features

Role of MMPs in tumor progression

MMP-2 biology and promoter polymorphisms

CANCER GENOMICS & PROTEOMICS 21: 502-510 (2024) doi:10.21873/cgp.20467 Impacts of Matrix Metalloproteinase-2 Promoter Genotypes on Breast Cancer Risk CHIH-CHIANG HUNG 1* , CHUNG-LIN TSAI2* , YU-TING CHIN3,4* , YUN-CHI WANG3,4 , CHIA-HUA LIU 1 , MENG-LIANG LIN5 , SHIH-SHUN CHEN6 , JIE-LONG HE7 , CHIA-WEN TSAI3,4 , CHEN-HSIEN SU4 , DA-TIAN BAU3,4,8 and WEN-SHIN CHANG3,4 1Division of Breast Surgery, Department of Surgery, Taichung Veterans General Hospital, Taichung, Taiwan, R. O. C. ; 2Division of Cardiac and Vascular Surgery, Cardiovascular Center, Taichung Veterans General Hospital, Taichung, Taiwan, R.O.C.; 3Graduate Institute of Biomedical Sciences, China Medical University, Taichung, Taiwan, R. O. C. ; 4Terry Fox Cancer Research Laboratory, Department of Medical Research, China Medical University Hospital, Taichung, Taiwan, R.O.C.; 5Department of Medical Laboratory Science and Biotechnology, China Medical University, Taichung, Taiwan, R.O.C.; 6Department of Medical Laboratory Science and Biotechnology, College of Medical and Health Science, Asia University, Taichung, Taiwan, R.O.C.; 7Department of Post-Baccalaureate Veterinary Medicine, Asia University, Taichung, Taiwan, R. O. C. ; 8Department of Bioinformatics and Medical Engineering, Asia University, Taichung, Taiwan, R. O. C. Abstract. Background/Aim: Matrix metalloproteinase-2 (MMP-2) has been implicated in the pathogenesis of breast cancer (BC) . However, there is limited research on the role of MMP-2 genotypes in BC risk. This study aimed to investigate the associations between two MMP-2 promoter polymorphisms, rs243865 and rs2285053, and BC risk. Materials and Methods: MMP-2 genotypes were analyzed using PCR-based RFLP methodology in a cohort comprising 1,232 BC cases and 1,232 controls. Results: Genotypic frequencies of MMP-2 rs243865 and rs2285053 in controls were consistent with Hardy-Weinberg equilibrium (p=0.3702 and 0.2036, respectively) . There were no significant *These Authors contributed equally to this study. Correspondence to: Da-Tian Bau and Wen-Shin Chang, Terry Fox Cancer Research Laboratory, Department of Medical Research, China Medical University Hospital, 2 Yuh-Der Road, Taichung, 404 Taiwan, R. O. C. Tel: +886 422053366 (Ext. 5805), e-mail: [artbau2@gmail.com](artbau2@gmail.com) (Bau), [halittlemelon@hotmail.com](halittlemelon@hotmail.com) (Chang) Key Words: Breast cancer, genotype, matrix metalloproteinase-2, polymorphism, triple negative breast cancer. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY-NC-ND) 4.0 international license ([https://creativecommons.org/licenses/by-nc-nd/4.0](https://creativecommons.org/licenses/by-nc-nd/4.0)). differences in the distribution of rs243865 and rs2285053 genotypes between BC cases and controls (p for trend=0.1602 and 0.2170, respectively) . Variant genotypesat rs243865 and rs2285053 appeared to confer a protective effect, although not statistically significant (all p>0.05) . Similarly, the variant T allele at rs243865 and rs2285053 showed a non-significant trend towards decreased BC risk (OR=0 .84 and 0. 89, 95%CI=0 .69-1.02 and 0.78-1. 02, p=0.0811 and 0.1043, respectively) . There was no interaction observed between MMP-2 rs243865 or rs2285053 genotypes and age. Stratified analysis did not reveal significant associations between MMP-2 rs243865 or rs2285053 genotypes and triple-negative breast cancer (TNBC) (p=0.6458 and 0.8745, respectively) . Among both TNBC and non-TNBC cases, none of the variant genotypesat rs243865 or rs2285053 showed significant associations with TNBC (all p>0.05) . Conclusion: MMP-2 rs243865 andrs2285053 genotypes appear to have a minimal impact on individual susceptibility to BC or TNBC. Breast cancer (BC) represents the most prevalent malignancy among women, profoundly impacting their physical and mental well-being in clinical settings. In 2020 alone, there were an alarmin

FAQs

Which MMP-2 promoter polymorphisms were examined in this study?

The study investigated MMP-2 promoter polymorphisms rs243865 and rs2285053 to assess their association with breast cancer risk.

How were MMP-2 genotypes analyzed?

MMP-2 genotypes were analyzed using a PCR-based RFLP methodology in a cohort of 1,232 breast cancer cases and 1,232 controls.

Did the genotypes rs243865 and rs2285053 significantly affect breast cancer or TNBC risk?

No significant differences were observed in genotype distribution between cases and controls, and stratified analysis did not reveal significant associations with triple-negative breast cancer (TNBC).

What was the overall conclusion about the impact of these genotypes?

MMP-2 rs243865 and rs2285053 genotypes appear to have only a minimal impact on individual susceptibility to breast cancer or TNBC.

Sep 21, 2026

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