H.S.C. (VOCATIONAL) MEDICAL LABORATORY TECHNICIAN - STD: XI (PAPER-3) - MICROBIOLOGY PRACTICAL HANDBOOK

Sep 27, 2026

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Microbiology Practical Handbook for H.S.C. (Vocational) Medical Laboratory Technician, STD: XI (Paper-3). Covers core practical work in microbiology laboratory operations, including sterilization and disinfection, preparation of culture media, hanging drop method, specimen collection procedures, and staining techniques such as Gram’s, Albert’s, and Z. N. staining. Also includes inoculation into media, isolation of organisms in pure culture, identification of microorganisms, bacteriology of water/air/milk, antibiotic sensitivity testing, fungal wet mount examination, and serology topics including ELISA and CRP. Provides laboratory safety and rules to support accurate, aseptic practice.

LanguageEnglish

CategoryExam

FormatPDF

Pages70

Reading Time2 h 56 min

AccessFree to download

Preface

Index

Operation of instruments and sterilization

Preparation of media

Hanging drop method

Specimen collection

Staining of bacteria

Inoculation into media

Isolation and identification of organisms

Bacteriology and antibiotic sensitivity

Fungal examination

Serology

H.S.C. (VOCATIONAL) MEDICAL LABORATORY TECHNICIAN STD: XI (PAPER-3) MICROBIOLOGY PRACTICAL HANDBOOK [ Price : Rs. 83 ] (iii) (iv) (v) (vii) PREFACE The national policy of education (1986) envisages that the introduction of systematic, well planned and rigorously implemented programme of vocational education is crucial in the proposed educational reorganization. In accordance with the policy of Govt. of India, State govt. of Maharashtra introduces +2 Vocationlization of Education in 1988- 89. During last 25 years no substantial efforts has been taken to revamp the curriculum. Ministry of Human Resource Development, Govt. of India developed the National Skill Qualification Framework (NSQF) to introduce vocational courses according to series of levels of knowledge & skills. Qualifications are made up of vocational standards for specific areas of learning units or units of competency. Units of competency are the specification of the knowledge and skill to the standard of performance expected in the workplace. The unit of competency or National Occupation Standards comprising generic and technical competencies an employee should possess is laid down by the Sector Skill Council of the respective economic or social sector. The challenges before us were to make smooth transition of curriculum from knowledge based to skill based and rapid technological changes in all sectors of economy. Hence, the few obsolete courses were either merge with core courses or deleted. Hence, in first phase 30 courses were converted into 20 courses. In second phase 20 more courses can be added sector wise as per National Occupational Standards. I acknowledge the hard team work done by District Vocational Education & Training Officer, who were the coordinators for curriculum designing, theory & practical books writing, along with the vocational teachers of various vocational field & experts from the industry. Shri.S.M.Haste, Joint Director & Shri.A. G. Gavit, Dy.Director has taken the sincere efforts from Directorate to produce the best text material with limited resources & time. J. D. Bhutange, Director (Vocational Education) Directorate of Vocational Education and Training, Mumbai, Maharashtra State (ix) INDEX | Sr. No. | Name of Practical | Page Nos. | | --- | --- | --- | | 1 to 4 | Operation of instruments . .
Sterilization and disinfection
• Disinfection in microbiology laboratory.
• Sterilization of given material in microbiology laboratory using moist heat.
• Sterilization of given material in microbiology laboratory using dry heat. | 2—7 | | 5 to 7 | Preparation of media
• Nutrient agar
• Blood agar
• Mac Conkey agar | 8—11 | | 8 | Hanging drop method . . | 12—13 | | 9 to 12 | Specimen collection
• Urine
• Blood
• Stool
• Throat swab and vaginal swab | 14—20 | | 13 to 15 | Staining of bacteria . .
• Gram’s staining ·
• Albert’s staining ·
• Z. N. staining | 21—26 | | 16 | Inoculation of clinical materials into media . .
- Continuous method | 27—28 | | 17 to 21 | Isolation of organisms in pure culture . .
• TSI
• IMViC test-Indole test
• IMViC test-MR test
• IMViC test-VP test
• IMViC test-Citrate test | 29—36 | | 22 Identification of microorganism . . 37—40
• Staphylococcus aureus
• Streptococcus pyogenes
• Pneumococcus
• Neisseria
• Escherichia coli
• Shigella
• Salmonella
• Proteus
• Vibrio cholera
• Corynebacteriun
• Clostridium welchii Ra 755—2 | | | (x) | Sr. No. | Name of Practical | Page Nos. | | --- | --- | --- | | | • Clostridium tetani
• Clostridium botulinum | | | 23 | Bacteriology of Water, Air, Milk . . | 41—42 | | 24 | Antibiotic sensitivity test . . | 43—44 | | 25 | Fungal examination by wet mount . .
(Lacto phenol cotton blue) | 45 | | 26 to 33 | SEROLOGY
• R.A. test
• VDRL test
• Widal test
• ELISA
• TORCH test
• Pregnancy test
• CRP | 46—62 | 1 INTRODUCTION TO M

FAQs

What safety rules must be followed in the microbiology laboratory?

Wear lab coat/apron, gloves, and shoes, use face masks where required, avoid bringing food or drink into the lab, and follow aseptic techniques. Wipe bench tops with disinfectant before and after work and promptly report accidents and dispose of contaminated materials as instructed.

Which practical topics are covered in the handbook’s index?

The index lists practicals such as operation of instruments, sterilization and disinfection, preparation of media, hanging drop method, specimen collection (urine, blood, stool, throat and vaginal swab), bacterial staining, inoculation into media, isolation in pure culture, identification of microorganisms, water/air/milk bacteriology, antibiotic sensitivity tests, fungal wet mount examination, and serology tests.

How does the handbook describe identification and diagnostic work in microbiology?

It explains that diagnostic microbiology laboratory work focuses on identifying infectious agents and providing information about the most effective antimicrobial agent and its dosage. Practical sections correspond to this through methods like staining, pure culture isolation, and identification of microorganisms.

Sep 27, 2026

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