High expression of centromere protein A and its molecular mechanism and clinical significance in prostate cancer: A study based on data mining and immunohistochemistry

Sep 24, 2026

The progression of prostate cancer (PCa) results in poor prognosis, while the molecular mechanism remains incompletely understood. This study aimed to clarify the key role of centromere protein A (CENPA) in PCa using bulk RNA-seq and in-house immunohistochemistry, single-cell RNA-seq across prostate subpopulations, enrichment analyses, and clinicopathological parameter evaluation. CENPA was upregulated in PCa and metastatic PCa, and overexpressed in cancer stem cells with androgen receptor-negative status, suggesting effects on cell cycle and metastasis.

LanguageEnglish

CategoryHealthcare

FormatPDF

Pages14

Reading Time35 min

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Abstract

Abbreviations

Introduction

DOI: 10.1049/syb2.12073 ORIGINAL RESEARCH High expression of centromere protein A and its molecular mechanism and clinical significance in prostate cancer: A study based on data mining and immunohistochemistry Fang‐Cheng Jiang1 | Gao‐Qiang Zhai2 | Jia‐Lin Liu2 | Rui‐Gong Wang2 | Yuan‐Ping Yang1 | Harivignesh Murugesan1 | Xiao‐Xiang Yu3 | Xiu‐Fang Du1 | Juan He1 | Zhen‐Bo Feng1 | Shang Ling Pan4 | Gang Chen1 | Sheng‐Hua Li2 | Zhi‐Guang Huang1 1Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning, China 2Department of Urology, The First Affiliated Hospital of Guangxi Medical University, Nanning, China 3Department of Urology, The 923rd Hospital of Chinese People's Liberation Army Joint Logistics Support Force, Nanning, China 4Department of Pathophysiology, School of Pre‐clinical Medicine, Guangxi Medical University, Nanning, Guangxi, China Correspondence Zhi‐Guang Huang, Department of Pathology, The First Affiliated Hospital of Guangxi Medical University, Nanning, China. Email: [huangzhiguang@gxmu.edu.cn](huangzhiguang@gxmu.edu.cn) Funding information Guangxi Medical University Undergraduate Innovation and Entrepreneurship Training Program, Grant/Award Number: 202010598159, 202010598087, 202110598060; Future Academic Star of Guangxi Medical University, Grant/Award Number: WLXSZX21115, WLXSZX21036, WLXSZX20083; The first Affiliated Hospital of Guangxi Medical University 2020 Undergraduate Innovation and Entrepreneurship Training Program, Grant/Award Number: 2020‐33, 2020‐7; Guangxi Educational Science Planning Key Project, Grant/ Award Number: 2021B167; the Fund of Natural Science Foundation of Guangxi, China, Grant/ Award Number: 2020AC03006; “Medical Excellence Award” Funded by the Creative Research Development Grant from the First Affiliated Hospital of Guangxi Medical University; Guangxi Clinical Research Center for Urology and Nephrology, Grant/Award Number: 2020AC03006; Guangxi Science and Technology Base and Talent Project, Grant/Award Number: 2019AC17009; Guangxi Zhuang Autonomous Region Health Abstract The progression of prostate cancer (PCa) leads to poor prognosis. However, the molecular mechanism of PCa is still not completely clear. This study aimed to elucidate the important role of centromere protein A (CENPA) in PCa. Large numbers of bulk RNA sequencing (RNA‐seq) data and in‐house immunohistochemistry data were used in analysing the expression level of CENPA in PCa and metastatic PCa (MPCa) . Single‐cell RNA‐seq data was used to explore the expression status of CENPA in different prostate subpopulations. Enrichment analysis was employed to detect the function of CENPA in PCa. Clinicopathological parameters analysis was utilised in analysing the clinical value of CENPA. The results showed that CENPA was upregulated in PCa (standardised mean difference [SMD] = 0.83, p = 0.001) and MPCa (SMD = 0.61, p = 0.029) . CENPA was overexpressed in prostate cancer stem cells (CSCs) with androgen receptor (AR) negative compared to epithelial cells with AR positive. CENPA may influence the development of PCa through affecting cell cycle. Patients with nodal metastasis had higher expression level of CENPA. And patients with high CENPA expression had poor disease‐free survival. Taken together, Overexpression of CENPA may influence the development of PCa by regulating cell cycle and promoting metastasis. Abbreviations: ADT, Androgen deprivation therapy.; AR, Androgen receptor; AUC, Area under curve; BP, Biological process; CC, Cellular component; ccRCC, Clear cell renal cell carcinoma; CENPA, Centromere protein; ChIP, Chromatin immunoprecipitation and DNA sequencing; CIN, chromosome instability; CSCs, Cancer stem cells; DEGs, Differentially expressed genes; GEO, Gene Expression Omnibus; IHC, Immunohistochemistry; IC50, half maximal inhibitory concentration; LPCa, Localised prostate cancer; MF, Molecular function; MPCa, Metastatic prostate cancer; PBS, Phosphate buf

FAQs

What is the main purpose of this study on CENPA in prostate cancer?

To elucidate the important role of centromere protein A (CENPA) in prostate cancer by integrating bulk RNA-seq, single-cell RNA-seq, enrichment analysis, and immunohistochemistry with clinicopathological evaluation.

How was CENPA expression assessed across prostate cancer stages and subpopulations?

Bulk RNA sequencing and immunohistochemistry were used to analyze CENPA expression levels in PCa and metastatic PCa, while single-cell RNA-seq was used to examine CENPA expression across different prostate subpopulations, including cancer stem cells.

What clinical significance is associated with high CENPA expression?

Higher CENPA expression correlated with nodal metastasis and worse disease-free survival, indicating potential value as a clinical marker and a contributor to disease progression through effects on cell cycle and metastasis.

Sep 24, 2026

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