Handbook of Analysis of Oligonucleotides and Related Products Jose

Jul 9, 2026

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Handbook of Analysis of Oligonucleotides and Related Products provides a structured reference for characterizing oligonucleotides and related materials. The contents outline analytical workflows for purity and impurities using reversed-phase and AEX HPLC, size-exclusion HPLC for molecular weight, and LC–mass spectrometry for identification. Additional chapters cover sequence determination with MS/MS and MALDI-TOF, Tm analysis, electrophoretic purity and content, immunoassay-based bioanalysis, potency and endotoxin testing, residual solvent analysis, and key physicochemical characterization methods including NMR, IR, stability-indicating approaches, and HILIC.

LanguageEnglish

CategoryResearch & Report

FormatPDF

Pages504

Reading Time21 h 10 min

Preface

Introduction

Purity analysis and impurities determination by reversed-phase HPLC

Purity analysis and impurities determination by AEX-HPLC

Purity analysis and molecular weight determination by size exclusion HPLC

Analysis by liquid chromatography and mass spectrometry

Sequence determination and confirmation by MS/MS and MALDI-TOF

Tm analysis of oligonucleotides

Purity and content analysis by capillary gel electrophoresis

Bioanalysis of therapeutic oligonucleotides using hybridization-based immunoassay

Oligonucleotide assay and potency

Microbial analysis: endotoxin testing

Analysis of residual solvents by headspace gas chromatography

Determination of extinction coefficient

Structural determination by NMR

Infrared analysis of oligonucleotides

Stability indicating methods for oligonucleotide products

Analysis by hydrophilic interaction chromatography

Handbook of Analysis of Oligonucleotides and Related Products E d it E d by J o s e V. B o n i l l a G. s usa n s r i Vat sa Handbook of Analysis of Oligonucleotides and Related Products E d it E d by J o s e V. B o n i l l a Girindus America, Cincinnati, Ohio, USA G. s usa n s r i Vat sa ElixinPharma, Encinitas, California, USA Boca Raton London New York CRC Press is an imprint of the Taylor & Francis Group, an informa business CRC Press Taylor & Francis Group 6000 Broken Sound Parkway NW, Suite 300 Boca Raton, FL 33487-2742 © 2011 by Taylor and Francis Group, LLC CRC Press is an imprint of Taylor & Francis Group, an Informa business No claim to original U.S. Government works Printed in the United States of America on acid-free paper 10 9 8 7 6 5 4 3 2 1 International Standard Book Number-13: 978-1-4398-1994-4 (Ebook-PDF) This book contains information obtained from authentic and highly regarded sources. Reasonable efforts have been made to publish reliable data and information, but the author and publisher cannot assume responsibility for the validity of all materials or the consequences of their use. The authors and publishers have attempted to trace the copyright holders of all material reproduced in this publication and apologize to copyright holders if permission to publish in this form has not been obtained. If any copyright material has not been acknowledged please write and let us know so we may rectify in any future reprint. Except as permitted under U.S. Copyright Law, no part of this book may be reprinted, reproduced, transmitted, or utilized in any form by any electronic, mechanical, or other means, now known or hereafter invented, including photocopying, microfilming, and recording, or in any information storage or retrieval system, without written permission from the publishers. For permission to photocopy or use material electronically from this work, please [access www.copyright.com](access www.copyright.com) ([http://](http://)[ ](http://)[www.copyright.com/](www.copyright.com/)) or contact the Copyright Clearance Center, Inc. (CCC), 222 Rosewood Drive, Danvers, MA 01923, 978-750-8400. CCC is a not-for-profit organization that provides licenses and registration for a variety of users. For organizations that have been granted a photocopy license by the CCC, a separate system of payment has been arranged. Trademark Notice: Product or corporate names may be trademarks or registered trademarks, and are used only for identification and explanation without intent to infringe. Visit the Taylor & Francis Web site at [http://www.taylorandfrancis.com](http://www.taylorandfrancis.com) and the CRC Press Web site at [http://www.crcpress.com](http://www.crcpress.com) Contents Preface................................................................................................................................................ v Editors ..............................................................................................................................................vii Contributors ......................................................................................................................................ix Introduction.......................................................................................................................................xi Chapter 1 Purity Analysis and Impurities Determination by Reversed-Phase High-Performance Liquid Chromatography ................................................................ 1 Hagen Cramer, Kevin J. Finn, and Eric Herzberg Chapter 2 Purity Analysis and Impurities Determination by AEX-HPLC ................................47 Jim Thayer, Veeravagu Murugaiah, and Yansheng Wu Chapter 3 Purity Analysis and Molecular Weight Determination by Size Exclusion HPLC...... 105 Ming Fai Chan and Ipsita Roymoulik Chapter 4 Analysis of Oligonucleotides by Liquid Chromatography and Mass Spe

FAQs

What analytical techniques are used to determine oligonucleotide purity and impurities?

Purity and impurities are addressed using reversed-phase high-performance liquid chromatography and AEX-HPLC, with additional separation approaches such as size exclusion HPLC and capillary gel electrophoresis.

How does the handbook support oligonucleotide sequence determination and confirmation?

Sequence determination and confirmation are covered using MS/MS and MALDI-TOF methods.

Which methods are described for physicochemical and stability-related characterization?

The contents include Tm analysis, determination of extinction coefficient, structural determination by NMR, infrared analysis, and stability-indicating methods for oligonucleotide products.

Jul 9, 2026

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