Fabrication of Fructooligosaccharide-Larimichthys crocea Peptide Copolymer - Wet-Heating-Derived Calcium Delivery and Bone-Lipid Metabolism Regulation

Sep 28, 2026

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Calcium delivery systems built from food-derived peptides and polysaccharides provide a strategy to improve calcium bioavailability while maintaining peptide activity. This study fabricates a fructooligosaccharide-Larimichthys crocea peptide (FOSLCP) copolymer using wet-heating and tests it as a multifunctional calcium carrier. FOS-LCP enhances calcium-binding, digestive stability, and resistance to dietary inhibitors, improving Ca2+ uptake and transport in intestinal in vitro models.

LanguageEnglish

CategoryHealthcare

FormatPDF

Pages17

Reading Time43 min

AccessFree to download

Abstract

Introduction

Calcium bioavailability and skeletal homeostasis

Dietary inhibitors and calcium absorption barriers

Peptide-polysaccharide copolymers for delivery

Methods and Evaluations

Calcium binding and digestive stability assessment

Intestinal uptake/transport models in vitro

Bone-lipid regulation using BMSCs

Results and Mechanistic Findings

Osteogenic and adipogenic differentiation effects

Wnt/β-catenin and PPARγ/C/EBPα pathway modulation

Adipokine secretion and lipolytic gene expression

Conclusion

Food Science & Nutrition | ORIGINAL ARTICLE OPEN ACCESS
Selected Papers From the 4th International Congress on Natural Products and Sustainable Development (SNDD 2025)
Fabrication ofFructooligosaccharide-Larimichthys crocea Peptide Copolymer by Wet-Heating for Enhanced Calcium Delivery and Bone-Lipid Metabolism Regulation
Chunlei Liu1 | Xiaoping Wu1,2 | Yihang Guo1 | Dan Li1,2
1College of Marine Science, Ningde Normal University, Ningde, China | 2Fujian Provincial University Engineering Research Center for Deep Processing of Mindong Aquatic Products, Ningde, China
Correspondence: Xiaoping Wu ([wuxiaoping1216@163.com](wuxiaoping1216@163.com))
Received: 3 June 2025 | Revised: 9 December 2025 | Accepted: 19 December 2025
Keywords: adipogenesis | calcium absorption | calcium delivery system | osteogenesis | physicochemical characteristics | wet-heating | | | --- | --- | | ABSTRACT
Calcium delivery systems based on food-derived peptides and polysaccharides offer a promising strategy for improving calcium bioavailability while preserving peptide bioactivity. In this study, a fructooligosaccharide-Larimichthys crocea peptide (FOSLCP) copolymer was fabricated by wet-heating and evaluated as a multifunctional calcium carrier. FOS-LCP showed improved calcium-binding capacity, digestive stability, and resistance to dietary inhibitors, thus increasing Ca2+ uptake and transport in intestinal models in vitro. Bone marrow mesenchymal stem cells isolated from calcium–deficient mice were used to assess bone-lipid regulation in vitro. In the presence of Ca2+, FOS-LCP promoted osteogenic differentiation and osteogenic marker expression, suppressed adipogenic differentiation, activated the Wnt/β-catenin pathway, inhibited PPARγ/C/EBPα-driven lipogenesis, reduced adipokine secretion, and enhanced lipolytic gene expression. These findings indicate that FOS-LCP coordinately enhances calcium absorption and rebalances bone-lipid metabolism at the cellular level, supporting wet-heating-derived peptidepolysaccharide copolymers as promising functional calcium delivery systems. | | | 1 | Introduction | insufficient dietary intake and poor calcium bioavailability (Sun, Zhang, et al. 2020; Wu et al. 2022) . Intestinal anti–nutritional | | Calcium is essential for skeletal homeostasis, with approxi- | factors, such as phytates (PA), oxalates (OA), and phosphates | | mately 99% of body calcium stored in the bones, which slows | (PPA), readily form insoluble complexes with calcium, thereby | | bone mineral density loss and helps prevent bone diseases (Wu et al. 2022; Zhu et al. 2020) . Nevertheless, approximately 51% of | reducing calcium absorption and contributing to bone-related disorders (Wu, Wang, et al. 2023) . Therefore, developing func- | | the global population is at risk of calcium deficiency owing to | tional delivery systems that can overcome intestinal absorption | | Abbreviations: ACC, acetyl-CoA carboxylase; ALP, alkaline phosphatase; aP2, adipocyte fatty-acid binding protein; ATGL, adipose triglyceride lipase; BGP,β-glycerophosphate; BMSCs, bone marrow mesenchymal stem cells; C/EBPα, CCAAT/enhancer-binding protein α; CaMKII, Ca2+/calmodulin-dependent protein kinase II; Col–I, type I collagen; CPPs, casein phosphopeptides; CPT1, carnitine palmitoyltransferase 1; DEX, dexamethasone; DKK1, dickkopf-1; DMT1, divalent metal transporter-1; FAS, fatty acid synthase; FOS, fructooligosaccharides; GPC, gel permeation chromatography; GSK–3β, glycogen synthase kinase-3β; HSL, hormone-sensitive lipase; IBMX, 3-isobutyl-1-methylxanthine; ICP–OES, inductively coupled plasma-optical emission spectrometry; LCP, Larimichthys crocea peptides; LRP5/6, low-density lipoprotein receptor-related protein 5/6; MMP7, matrix metalloproteinase–7; MW, molecular weight; OA, oxalate; OCN, osteocalcin; OPA, ortho-phthalaldehyde; PA, phytate; PKC, protein kinase C; PPA, phosphate; PPARγ, peroxisome proliferator-activated receptors γ; RT-qPCR, rev

FAQs

How was the fructooligosaccharide-Larimichthys crocea peptide copolymer prepared?

The FOSLCP copolymer was fabricated by wet-heating, combining a fructooligosaccharide component with Larimichthys crocea peptide.

What benefits does FOS-LCP show for calcium handling?

FOS-LCP exhibits improved calcium-binding capacity, digestive stability, and resistance to dietary inhibitors, leading to increased Ca2+ uptake and transport in intestinal in vitro models.

How does FOS-LCP influence bone and fat-related processes in vitro?

In calcium-present conditions, FOS-LCP promotes osteogenic differentiation and osteogenic marker expression while suppressing adipogenic differentiation, with Wnt/β-catenin activation and inhibition of PPARγ/C/EBPα-driven lipogenesis.

Sep 28, 2026

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