Expression and significance of pigment epithelium-derived factor and vascular endothelial growth factor in colorectal adenoma and cancer

Sep 24, 2026

Uploaded by Riley

Incidence and mortality of colorectal cancer rank among the highest worldwide, with tumor progression closely linked to angiogenesis and the imbalance between PEDF (anti-angiogenic) and VEGF (pro-angiogenic). This case-control study investigated PEDF, VEGF, and CD31-stained microvessel density across normal mucosa, colorectal adenoma, and CRC. Archived specimens (April–October 2022) underwent immunohistochemistry, with statistical comparisons and correlation analyses to define expression patterns and clinicopathological associations.

LanguageEnglish

CategoryHealthcare

FormatPDF

Pages18

Reading Time45 min

AccessFree to download

Abstract

Background

Aim

Methods

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| W | J | G | O | World Journal of
Gastrointestinal Oncology | | --- | --- | --- | --- | --- | Submit a Manuscript: [https://www.f6publishing.com](https://www.f6publishing.com) World J Gastrointest Oncol 2024 March 15; 16(3): 670-686 DOI: 10.4251/wjgo.v16.i3.670 ISSN 1948-5204 (online) ORIGINAL ARTICLE Case Control Study Expression and significance of pigment epithelium-derived factor and vascular endothelial growth factor in colorectal adenoma and cancer Ye Yang, Wu Wen, Feng-Lin Chen, Ying-Jie Zhang, Xiao-Cong Liu, Xiao-Yan Yang, Shan-Shan Hu, Ye Jiang, Jing Yuan Specialty type: Gastroenterology and hepatology Provenance and peer review: Unsolicited article; Externally peer reviewed. Peer-review model: Single blind Peer-review report’s scientific quality classification Grade A (Excellent): 0 Grade B (Very good): B Grade C (Good): 0 Grade D (Fair): 0 Grade E (Poor): 0 P-Reviewer: Šarenac TM, Serbia Received: November 7, 2023 Peer-review started: November 7, 2023 First decision: December 31, 2023 Revised: January 16, 2024 Accepted: February 4, 2024 Article in press: February 4, 2024 Published online: March 15, 2024 Ye Yang, Digestive Diseases, Chengdu Qingbaijiang District People's Hospital, Chengdu 610300, Sichuan Province, China Wu Wen, Ying-Jie Zhang, Xiao-Cong Liu, Xiao-Yan Yang, Shan-Shan Hu, Ye Jiang, Jing Yuan, Digestive Diseases, Chengdu Second People’s Hospital, Chengdu 610000, Sichuan Province, China Feng-Lin Chen, Graduate School, Chengdu Medical College, Chengdu 610000, Sichuan Province, China Corresponding author: Wu Wen, Doctor, Chief Physician, Digestive Diseases, Chengdu Second People’s Hospital, No. 10 Qingyunan Street, Jinjiang District, Chengdu 610000, Sichuan Province, [China.](China. wenwu2@qq.com)[ wenwu2@qq.com](China. wenwu2@qq.com) Abstract BACKGROUND The incidence and mortality of colorectal cancer (CRC) are among the highest in the world, and its occurrence and development are closely related to tumor neovascularization. When the balance between pigment epithelium-derived factors (PEDF) that inhibit angiogenesis and vascular endothelial growth factors (VEGF) that stimulate angiogenesis is broken, angiogenesis is out of control, resulting in tumor development. Therefore, it is very necessary to find more therapeutic targets for CRC for early intervention and later treatment. AIM To investigate the expression and significance of PEDF, VEGF, and CD31-stained microvessel density values (CD31-MVD) in normal colorectal mucosa, adenoma, and CRC. METHODS In this case-control study, we collected archived wax blocks of specimens from the Digestive Endoscopy Center and the General Surgery Department of Chengdu Second People's Hospital from April 2022 to October 2022. Fifty cases of specimen wax blocks were selected as normal intestinal mucosa confirmed by electronic colonoscopy and concurrent biopsy (normal control group), 50 cases of specimen WJGO [https://www.wjgnet.com](https://www.wjgnet.com) 670 March 15, 2024 Volume 16 Issue 3 Yang Y et al. PEDF and VEGF in CRC wax blocks were selected as colorectal adenoma confirmed by electronic colonoscopy and pathological biopsy (adenoma group), and 50 cases of specimen wax blocks were selected as CRC confirmed by postoperative pathological biopsy after inpatient operation of general surgery (CRC group) . An immunohistochemical staining experiment was carried out to detect PEDF and VEGF expression in three groups of specimens, analyze their differences, study the relationship between the two and clinicopathological factors in CRC group, record CD31-MVD in the three groups, and analyze the correlation of PEDF, VEGF, and CD31-MVD in the colorectal adenoma group and the CRC group. The F test or adjusted F test is used to analyze measurement data statistically. KruskalWallis rank sum test was used between groups for ranked data. The chi-square test, adjusted chi-square test, or Fisher's e

FAQs

What was the purpose of comparing PEDF, VEGF, and CD31-MVD across groups?

To determine how PEDF, VEGF, and CD31-stained microvessel density differ between normal colorectal mucosa, colorectal adenoma, and colorectal cancer, and to assess their significance.

How were specimens selected and what experimental method was used?

Archived wax blocks from April 2022 to October 2022 were selected into normal, adenoma, and CRC groups. Immunohistochemical staining was used to detect PEDF and VEGF expression.

What were the main findings about PEDF and VEGF expression?

PEDF expression decreased progressively from normal mucosa to adenoma to CRC, while VEGF expression increased across the same groups. PEDF and VEGF also showed associations with CRC differentiation, invasion, metastasis, and TNM stage.

Sep 24, 2026

Uploaded by Riley