Establishing a new human lung squamous cell carcinoma cell line, OMUL-1, expressing insulin-like growth factor 1 receptor and programmed cell death ligand 1

Sep 21, 2026

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Squamous cell carcinoma is the second most prevalent type of non–small cell lung cancer, and progress in molecular mechanism research requires reliable squamous lung cancer cell models. A novel lung squamous cell line, OMUL-1, was generated from primary tumor tissue of a 74-year-old man, and its growth behavior, tumorigenicity, histology, gene expression, and invasion were characterized using RT-PCR, RNA sequencing, and functional assays. OMUL-1 formed tumors in mice, showed squamous histology, expressed multiple receptor/kinase genes, and responded to an IGF1R inhibitor; IGF1R and PD-L1 were detected in primary and xenograft tumors.

LanguageEnglish

CategoryHealthcare

FormatPDF

Pages9

Reading Time23 min

AccessFree to download

Abstract

Background

Methods

Results

Conclusions

Introduction

Epidemiology and unmet needs

Role of PD-L1 and ICIs

Need for squamous cell line tools

Methods

Patient

DOI: 10.1111/1759-7714.15488 ORIGINA L ARTICLE Establishing a new human lung squamous cell carcinoma cell line, OMUL-1, expressing insulin-like growth factor 1 receptor and programmed cell death ligand 1 Hiroaki Nagamine 1 | Masakazu Yashiro 2 | Megumi Mizutani 1 | Akira Sugimoto 1 | Yoshiya Matsumoto 1 | Yoko Tani 3 | Hiroyasu Kaneda 3 | Kazuhiro Yamada 1 | Tetsuya Watanabe 1 | Kazuhisa Asai 1 | Satoshi Suzuki 4 | Tomoya Kawaguchi 1,3 1Department of Respiratory Medicine, Graduate School of Medicine, Osaka Metropolitan University, Osaka, Japan 2Molecular Oncology and Therapeutics, Graduate School of Medicine, Osaka Metropolitan University, Osaka, Japan 3Department of Clinical Oncology, Graduate School of Medicine, Osaka Metropolitan University, Osaka, Japan 4Department of Thoracic Surgery, Graduate School of Medicine, Osaka Metropolitan University, Osaka, Japan Correspondence Masakazu Yashiro, Molecular Oncology and Therapeutics, Osaka Metropolitan University, Graduate School of Medicine, 1-4-3 Asahimachi, Abeno-ku, Osaka 545-8585, Japan. Email: [i21496f@omu.ac.jp](i21496f@omu.ac.jp) Abstract The Main Problem: Squamous cell carcinoma is the second most prevalent type of non–small cell lung cancer. Analyzing the molecular mechanisms underlying lung carcinoma requires useful tools, such as squamous lung cancer cell lines. Methods: A novel new lung squamous cell carcinoma cell line, OMUL-1, was developed from the primary lung cancer of a 74-year-old man. We assessed the characteristics and behavior of OMUL-1 cells were examined, including their growth kinetics, tumorigenicity in mice, histological properties, gene expression profiles using reverse transcription polymerase chain reaction (RT-PCR), and RNA sequencing and invasion assays. Results: OMUL-1-an adherent cell line-resulted in 100% tumor formation when subcutaneously injected into mice. Histological analysis of the subcutaneous tumor using hematoxylin and eosin staining revealed squamous cell carcinoma with characteristics similar to those of the primary tumor (p40 and p63 were positive, and TTF-1 was negative) . An invasion assay demonstrated that OMUL-1 had a lower invasion ability compared to that of other developed cell lines. RT-PCR analysis and RNA sequencing indicated that OMUL-1 cells expressed FGFR1, FGFR2, FGFR3, FGFR4, EGFR, HER2, ErbB3, ErbB4, VEGFR3, IGF1R, c-MET, PDGFRa, and PDGFRb. Additionally, picropodophyllin (an IGF1R inhibitor) significantly inhibited the growth of OMUL-1 cells. Immunohistochemistry revealed that IGF1R and PD-L1 were expressed in both the primary and subcutaneous tumors. Conclusions: We developed a novel new squamous cell lung carcinoma cell line, OMUL-1, that expresses IGF1R and PD-L1 . KEYW ORD S cell line, IGF1R, lung cancer, PD-L1, squamous cell carcinoma INTRODUCTION Lung cancer is the primary cause of cancer-related deaths worldwide.1 Non–small cell lung cancer (NSCLC) accounts for 85% of all lung cancers. Squamous cell carcinoma is the second most prevalent type and accounts for 30% of all NSCLC cases, followed by adenocarcinoma.2 Owing to its high frequency and poor prognosis, understanding the This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. © 2024 The Author(s) . Thoracic Cancer published by John Wiley & Sons Australia, Ltd. underlying mechanisms of carcinogenesis, proliferation, and metastasis in lung squamous cell carcinoma and the development of novel treatment strategies are crucial. Various driver genes, such as epidermal growth factor receptor (EGFR)3 and anaplastic lymphoma kinase (ALK),4 have been identified in NSCLC. Additionally, their inhibitors are promising therapeutic agents of adenocarcinoma.5–8 However, molecularly targeted inhibit

FAQs

Why was a new squamous lung cancer cell line needed?

Squamous cell carcinoma is common and has poor outcomes, and studying molecular mechanisms requires useful squamous lung cancer cell lines as research tools. The document notes that only a limited number of squamous lung carcinoma cell lines exist worldwide.

How was OMUL-1 established and what patient information is described?

OMUL-1 was derived from the primary lung cancer of a 74-year-old Japanese man with a long smoking history. Imaging and biopsy confirmed squamous cell carcinoma, and surgery including lower lobectomy and mediastinal lymph node dissection was performed.

What key biological characteristics did OMUL-1 show?

OMUL-1 formed tumors in mice with 100% subcutaneous tumor formation. Histology indicated squamous cell carcinoma, invasion ability was lower than other developed cell lines, and gene expression profiles showed multiple receptors/kinases including IGF1R; picropodophyllin inhibited OMUL-1 growth.

Sep 21, 2026

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