EasySep™ Human Whole Blood Myeloid Positive Selection Kit - Catalog 18683 - Version 1.2.3

Q: What storage conditions and shelf-life guidance are provided for kit components?

The selection cocktail and magnetic nanoparticles are stored at 2–8°C and must not be frozen, while the RBC lysis buffer concentrate is stored at 15–25°C and must not be frozen. Stability is described as stable until the expiry date (EXP) on each label, with additional guidance for 1X dilution of RBC lysis buffer stored at 2–8°C.

Oct 3, 2026

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EasySep™ Human Whole Blood Myeloid Positive Selection Kit provides immunomagnetic positive selection to isolate highly purified whole blood myeloid cells from fresh samples. Cells are labeled using antibodies recognizing CD33 and CD66b surface markers and magnetic particles, enabling separation without columns by an EasySep™ magnet. Isolated cells are available for downstream flow cytometry, culture, or DNA/RNA extraction. The kit includes defined cocktail, nanoparticles, and 10X RBC lysis buffer with specified storage and stability.

LanguageEnglish

CategoryHealthcare

FormatPDF

Pages4

Reading Time10 min

AccessFree to download

Component Descriptions

Additional Reagent Stability Information

Sample Preparation

Peripheral Blood

Recommended Medium

Directions for Use – Manual EasySep™ Protocols

Table 1 Protocol Steps

Directions for Use – Fully Automated RoboSep™ Protocol

Table 2 Protocol Steps

This document [is available at www.stemcell.com/PIS](is available at www.stemcell.com/PIS) EasySep™ Human Whole Blood Myeloid Positive Positive Selection Selection Kit Catalog \#18683 For processing 60 mL whole blood Document \#28986 | Version 1_2_3 Description Isolate highly purified whole blood myeloid cells from fresh whole blood by immunomagnetic positive selection. 􀁸 Fast and easy-to-use 􀁸 Up to 99% purity 􀁸 No columns required This kit targets whole blood myeloid cells for positive selection with an antibody recognizing the CD33 and CD66b surface markers. Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, or DNA/RNA extraction. Component Descriptions | COMPONENT NAME | COMPONENT \# | QUANTITY | STORAGE | SHELF LIFE | FORMAT | | --- | --- | --- | --- | --- | --- | | EasySep™ Human Whole Blood Myeloid Positive Selection Cocktail | 18683C | 3 x 1 mL | Store at 2-8°C.
Do not freeze. | Stable until expiry date (EXP) on label. | A combination of monoclonal antibodies in PBS. | | EasySep™ Whole Blood Magnetic Nanoparticles | 18180 | 3 x 1 mL | Store at 2-8°C.
Do not freeze. | Stable until expiry date (EXP) on label. | A suspension of magnetic particles in water. | | EasySep™ Red Blood Cell Lysis Buffer, 10X Concentrate | 20110 | 1 x 10 mL | Store at 15-25°C. Do not freeze. | Stable until expiry date (EXP) on label. | A 10X concentrated red blood cell lysis reagent. | PBS-phosphate-buffered saline Components may be shipped at room temperature (15-25°C) but should be stored as indicated above. Additional Reagent Stability Information | REAGENT NAME | STORAGE | SHELF LIFE | | --- | --- | --- | | EasySep™ Red Blood Cell Lysis Buffer (1X dilution) | Store at 2-8°C. Do not freeze. | Stable for up to 3 months. Do not exceed the expiry date (EXP) of the original component. | Sample Preparation For available fresh and frozen samples, [see www.stemcell.com/primarycells](see www.stemcell.com/primarycells). PERIPHERAL BLOOD Collect whole blood in a blood collection tube containing anticoagulant. Recommended Medium EasySep™ Buffer (Catalog \#20144), RoboSep™ Buffer (Catalog \#20104) , or PBS containing 2% fetal bovine serum (FBS) and 1 mM EDTA. Medium should be free of Ca++ and Mg++ . |
EasySep™ Human Whole Blood Myeloid Positive Selection Kit
Positive Selection | | | | | --- | --- | --- | --- | | Directions for Use – Manual EasySep™ Protocols
See page 1 for Sample Preparation and Recommended Medium. Refer to Table 1 for detailed instructions regarding the EasySep™ procedure.
Table 1. EasySep™ Human Whole Blood Myeloid Positive Selection Kit Protocol | | | | | | | EASY SEP™ MAGNET | | | STEP | INSTRUCTIONS | “The Big Easy”
(Catalog \#18001) | | | 1 | Prepare sample within the volume range. | Up to 4.5 mL | | | | Add sample to required tube. | 14 mL (17 x 100 mm) polystyrene round-bottom tube (e.g. Corning Catalog \#352057) | | | 2 | Add 1X EasySep™ RBC Lysis Buffer to sample. | Equal volume to sample | | | 3 | Add Selection Cocktail to sample. | 25 μL/mL of diluted sample | | | | Mix and incubate. | RT for 15 minutes | | | 4 | Mix Magnetic Particles.
NOTE: Particles should appear evenly dispersed. | Pipette up and down more than 5 times | | | 5 | Add Magnetic Particles to sample. | 25 μL/mL of diluted sample | | | | Mix and incubate. | RT for 10 minutes | | | 6 | Add recommended medium to top up the sample to the indicated volume. Mix by gently pipetting up and down 2 3 times. | 􀁸 Top up to 5 mL for samples < 2.5 mL
􀁸 Top up to 10 mL for samples ≥ 2.5 mL | | | | Place the tube (without lid) into the magnet and incubate. | RT for 10 minutes | | | 7 | Pick up the magnet, and in one continuous motion

FAQs

What storage conditions and shelf-life guidance are provided for kit components?

The selection cocktail and magnetic nanoparticles are stored at 2–8°C and must not be frozen, while the RBC lysis buffer concentrate is stored at 15–25°C and must not be frozen. Stability is described as stable until the expiry date (EXP) on each label, with additional guidance for 1X dilution of RBC lysis buffer stored at 2–8°C.

Oct 3, 2026

Upload by วิน