Dual-specificity phosphatase 14 inhibits the growth of cervical cancer cells by regulating the transforming growth factor-beta-activated kinase 1 and NOD-like receptor pyrin domain containing 3 inflammatory pathways

Q: What was the main objective of the study?

To investigate the role and mechanism of dual-specificity phosphatase 14 in cervical cancer.

Q: What effects did DUSP14 overexpression have on cervical cancer cells?

It significantly inhibited cell viability, induced apoptosis, and suppressed cell migration in HeLa and SiHa cells.

Sep 23, 2026

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This experimental study examines how dual-specificity phosphatase 14 (DUSP14) influences cervical cancer progression and the underlying mechanism. Clinical samples from five cervical squamous cell carcinoma patients and five chronic cervicitis patients were analyzed, alongside DUSP14 overexpression in HeLa and SiHa cells. Gene and protein changes were assessed by qRT-PCR and western blotting, while viability, apoptosis, and migration were measured using CCK-8, flow cytometry, Transwell, and wound healing assays.

LanguageEnglish

CategoryHealthcare

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Pages18

Reading Time45 min

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Abstract

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Introduction

Dual-specificity phosphatase 14 inhibits the growth of cervical cancer cells by regulating the transforming growth factor-beta-activated kinase 1 and NOD-like receptor pyrin domain containing 3 inflammatory pathways Yuanyuan Zhang , Qian Zhuo, Shaliya Abuduwufu and Aerziguli Wushouer Journal of International Medical Research 2026, Vol. 54(4) 1–18! The Author(s) 2026 Article reuse guidelines: [sagepub.com/journals-permissions](sagepub.com/journals-permissions)[ ](sagepub.com/journals-permissions)[DOI: 10.1177/03000605261441200](DOI: 10.1177/03000605261441200)[ ](DOI: 10.1177/03000605261441200)[journals.sagepub.com/home/imr](journals.sagepub.com/home/imr) Abstract Objective: This study investigated the role and mechanism of dual-specificity phosphatase 14 in cervical cancer. Methods: In this experimental study, clinical samples from five patients with cervical squamous cell carcinoma and five with chronic cervicitis were analyzed. Dual-specificity phosphatase 14 was overexpressed in HeLa and SiHa cells. Gene and protein expression levels were analyzed using real-time quantitative polymerase chain reaction and western blot analysis. Cell viability, apoptosis, and migration were assessed using cell counting kit-8, flow cytometry as well as Transwell and wound healing assays. Cytokine levels were measured using enzyme-linked immunosorbent assay. Results: Dual-specificity phosphatase 14 expression was significantly downregulated in cervical cancer tissues and cell lines compared with that in their respective controls (p <0.05). Dualspecificity phosphatase 14 overexpression markedly inhibited cell viability and induced apoptosis in HeLa and SiHa cells (p <0.05). Furthermore, it significantly suppressed cell migration (p <0.05). At the molecular level, dual-specificity phosphatase 14 upregulation decreased The Fifth Department of Gynecological Surgery, the Affiliated Tumor Hospital of Xinjiang Medical University, P. R. China Corresponding author: Yuanyuan Zhang, the Fifth Department of Gynecological Surgery, the Affiliated Tumor Hospital of Xinjiang Medical University, No. 789, Suzhou East Street, Urumqi 830000, P. R. China. Email: [519602409@qq.com](519602409@qq.com) Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License ([https://creativecommons.org/licenses/by-nc/4.0/](https://creativecommons.org/licenses/by-nc/4.0/)) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages ([https://us.sagepub.com/en-us/nam/open-access-at-sage](https://us.sagepub.com/en-us/nam/open-access-at-sage)). messenger ribonucleic acid levels of transforming growth factor-beta-activated kinase 1, NOD-like receptor pyrin domain containing 3, interleukin-1 beta, and tumor necrosis factoralpha; reduced protein levels of phosphorylated transforming growth factor-beta-activated kinase 1 and NOD-like receptor pyrin domain containing 3; and diminished secretion of interleukin-1 beta and tumor necrosis factor-alpha cytokines (p <0.05). Conclusion: Dual-specificity phosphatase 14 acts as a tumor suppressor in cervical cancer by inhibiting proliferation and migration, promoting apoptosis, and suppressing the transforming growth factor-beta-activated kinase 1/NOD-like receptor pyrin domain containing 3 inflammatory pathway, highlighting its potential as a novel therapeutic target and biomarker. Keywords Cervical cancer, dual-specificity phosphatase 14, apoptosis, transforming growth factorbeta-activated kinase 1, NOD-like receptor pyrin domain containing 3 Date received: 17 September 2025; accepted: 25 March 2026 Introduction Cervical cancer is one of the most common types of cancer responsible for morbidity and mortality in women worldwide. According to data from the World Health Organization, approximate

FAQs

What was the main objective of the study?

To investigate the role and mechanism of dual-specificity phosphatase 14 in cervical cancer.

How was DUSP14 manipulated in the experiments?

Dual-specificity phosphatase 14 was overexpressed in HeLa and SiHa cervical cancer cells.

What effects did DUSP14 overexpression have on cervical cancer cells?

It significantly inhibited cell viability, induced apoptosis, and suppressed cell migration in HeLa and SiHa cells.

Sep 23, 2026

Uploaded by นรินทร์