Differences in diversity and composition of mucosa-associated colonic microbiota in colorectal cancer and non-colorectal cancer in Indonesia

Sep 23, 2026

Colorectal cancer is a leading global malignancy with gut microbiota increasingly recognized as a contributing factor. This case-control study assessed colonic mucosal microbiota diversity and composition in Indonesian patients with colorectal cancer versus non-colorectal cancer. Sixty-nine 16S rRNA-based microbiota profiles were generated using 16S rRNA sequencing and analyzed through wf-metagenomics pipeline. Distinct compositional differences emerged in beta diversity at genus and species levels, with specific taxa enriched in each group, indicating a colorectal cancer-associated mucosal microbial signature.

LanguageEnglish

CategoryHealthcare

FormatPDF

Pages17

Reading Time43 min

AccessFree to download

Abstract

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| W | J | G | World Journal of
Gastroenterology | | --- | --- | --- | --- | Submit a Manuscript: [https://www.f6publishing.com](https://www.f6publishing.com) World J Gastroenterol 2025 February 21; 31(7): 100051 DOI: 10.3748/wjg.v31.i7.100051 ISSN 1007-9327 (print) ISSN 2219-2840 (online) ORIGINAL ARTICLE Case Control Study Differences in diversity and composition of mucosa-associated colonic microbiota in colorectal cancer and non-colorectal cancer in Indonesia Nikko Darnindro, Murdani Abdullah, Ninik Sukartini, Cleopas M Rumende, Amanda Pitarini, Saskia A Nursyirwan, Achmad Fauzi, Dadang Makmun, Erni J Nelwan, Hamzah Shatri, Ikhwan Rinaldi, Caroline Tanadi Specialty type: Gastroenterology and hepatology Provenance and peer review: Unsolicited article; Externally peer reviewed. Peer-review model: Single-blind Peer-review report’s classification Scientific Quality: Grade C, Grade C, Grade C Novelty: Grade B, Grade B, Grade C Creativity or Innovation: Grade B, Grade B, Grade C Scientific Significance: Grade B, Grade B, Grade B P-Reviewer: Kumar A; Wang ZC; Wu HM Received: August 6, 2024 Revised: December 5, 2024 Accepted: December 23, 2024 Published online: February 21, 2025 Processing time: 167 Days and 1.4 Hours Nikko Darnindro, Murdani Abdullah, Amanda Pitarini, Saskia A Nursyirwan, Achmad Fauzi, Dadang Makmun, Division of Gastroenterology, Pancreatobiliary and Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia-Cipto Mangunkusumo National General Hospital, Jakarta 10430, Indonesia Nikko Darnindro, Division of Gastrohepatology, Department of Internal Medicine, Fatmawati General Hospital, Jakarta 12430, Indonesia Murdani Abdullah, Human Cancer Research Center, IMERI Faculty of Medicine, Universitas Indonesia, Jakarta 10430, Indonesia Ninik Sukartini, Department of Clinical Pathology, Faculty of Medicine Universitas IndonesiaCipto Mangunkusumo National General Hospital, Jakarta 10430, Indonesia Cleopas M Rumende, Division of Respirology and Critical Care, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia-Cipto Mangunkusumo National General Hospital, Jakarta 10430, Indonesia Erni J Nelwan, Division of Tropical Medicine and Infectious Disease, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia-Cipto Mangunkusumo National General Hospital, Jakarta 10430, Indonesia Hamzah Shatri, Division of Psychosomatic and Palliative Medicine, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia-Cipto Mangunkusumo National General Hospital, Jakarta 10430, Indonesia Ikhwan Rinaldi, Division of Haematology and Medical Oncology, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia-Cipto Mangunkusumo National General Hospital, Jakarta 10430, Indonesia Caroline Tanadi, School of Medicine and Health Sciences, Atma Jaya Catholic University of Indonesia, Jakarta 14440, Indonesia Corresponding author: Nikko Darnindro, MD, Division of Gastroenterology, Pancreatobiliaryand Digestive Endoscopy, Department of Internal Medicine, Faculty of Medicine Universitas Indonesia-Cipto Mangunkusumo National General Hospital, Jl. Pangeran Diponegoro No. 71, WJG [https://www.wjgnet.com](https://www.wjgnet.com) 1 February 21, 2025 Volume 31 Issue 7 Darnindro N et al. Mucosal colonic microbiota in colorectal cancer in Indonesia Kenari, Kec. Senen, Kota Jakarta Pusat, Daerah Khusus Ibukota, Jakarta 10430, [Indonesia.](Indonesia. nikkodarnindro@gmail.com)[ nikkodarnindro@gmail.com](Indonesia. nikkodarnindro@gmail.com) Abstract BACKGROUND Colorectal cancer is the third most common malignancy and the fourth leading cause of cancer-related deaths worldwide. Several studies have shown an association between gut microbiota and colorectal cancer. Gut microbiota is unique and can be influenced by geographic factors and habits. This study aimed to determine the divers

FAQs

What was the main purpose of this case-control study?

To determine the diversity and composition of colonic mucosa-associated microbiota in Indonesian patients with and without colorectal cancer.

How were the microbiota samples analyzed?

Microbiota examination used 16S rRNA sequencing, and bioinformatics analysis was performed with the wf-metagenomics pipeline from EPI2Me-Labs.

Which microbiota features differed between colorectal and non-colorectal cancer groups?

Significant beta diversity differences were observed at genus and species levels; several phyla and specific genera/species showed higher prevalence or relative abundance in colorectal cancer versus non-colorectal cancer.

Sep 23, 2026

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