DecreasedSLC16A13Expression Level Can Increase Apoptosis in KATO2 Cell Line, A Promising Biomarker

Q: What was the objective of reducing SLC16A13 in KATO2 gastric cancer cells?

To determine how lowering SLC16A13 expression affects survival, proliferation, apoptosis, and metastatic-related potential in KATO2 cells.

Q: How were cell death and apoptosis evaluated after si-RNA transfection?

Cell death and apoptosis were measured using MTT and flow cytometry assays following SLC16A13 si-RNA transfection.

Q: Did decreased SLC16A13 expression affect cancer cell migration?

No. The study reported that reduced SLC16A13 expression did not impact KATO2 cell migration as evaluated by the scratch test.

Sep 21, 2026

Uploaded by Theodore

Gastric cancer remains a major cause of morbidity and mortality, and current tumor markers lack sufficient accuracy for early detection and reliable prognosis. This study reduces SLC16A13 expression in gastric cancer KATO2 cells using SLC16A13 si-RNA to evaluate effects on survival, proliferation, apoptosis, and migration. MTT, flow cytometry, scratch assay, western blot, and Real-Time PCR assess cell death, apoptosis, gene expression, and metastatic potential. Results show increased apoptosis and reduced viability, supporting SLC16A13 as a promising therapeutic biomarker.

LanguageEnglish

CategoryResearch & Report

FormatPDF

Pages6

Reading Time15 min

AccessFree to download

Abstract

Introduction

Materials and Methods

Cell Culture and Transfection

Cell Viability Assay (MTT)

RESEARCH ARTICLE Editorial Process: Submission:09/09/2023 Acceptance:06/14/2024 DecreasedSLC16A13Expression Level Can Increase Apoptosis in KATO2 Cell Line, A Promising Biomarker Samira Zavarpour1, Habib Zarredar2, Negar Pedram3, TorajAsvadi Kermani2, Zahra Soleimani2, Shahriyar Hashemzadeh2, AtefehAnsarin2, Mortaza Raeisi1 * Abstract Objective: Gastric cancer is a prevalent cancer type worldwide, and significant research efforts are focused on finding effective treatments. Recent studies have highlighted the importance of plasma membrane carriers, particularly solute carriers, in cancer progression. The SLC16A family, notably the SLC16A13 gene, plays a critical role in cancer development and tumor growth. This study aims to explore the impact of reducing SLC16A13 expression in gastric cancer cells on their survival, proliferation, and metastatic potential. Methods: Gastric cancer cells (KATO2) were cultured in RPMI medium supplemented with 10% fetal bovine serum. The cells were then transfected with SLC16A13 si-RNA to lower gene expression. The effects of this si-RNA on cell death and apoptosis were assessed using MTT and flow cytometry assays. Cell migration capabilities were evaluated using the scratch test. Western blot and Real-Time PCR were employed to measure SLC16A13 expression levels and protein detection. Additionally, RT-PCR was used to analyze changes in genes related to apoptosis and cell migration. Results: The reduction ofSLC16A13 expression following si-RNA transfection significantly increased apoptosis and cell death in the KATO2 cell line after 72 hours (P < 0.0001). Furthermore, the study revealed that decreased SLC16A13 expression did not impact cancer cell migration. Cell viability, assessed by MTT assay, showed a significant decrease at 48 and 72 hours post-transfection (P < 0.0001) . Conclusion: The findings indicate that targeting SLC16A13 can effectively increase cell death and apoptosis in gastric cancer cells, making it a viable therapeutic target. Keywords: Gastric cancer-apoptosis-SLC16A13-si-RNA-real time-PCR Asian Pac J Cancer Prev, 25 (6), 1953-1958 Introduction According to the International Cancer Society’s 2018 statistics report, gastric cancer remains the fifth most commonly diagnosed cancer in hospitals globally and the third leading cause of cancer-related deaths as of 2020. Gastric cancer (GC) arises from a confluence of genetic and environmental factors, such as a familial predisposition, unhealthy dietary habits, frequent alcohol consumption, smoking, Helicobacter pylori infection, and Epstein-Barr virus (EBV) exposure [1, 2] . At present, the main tumor markers utilized within the clinic for early detection of GC are not very accurate, so they cannot be used to definitively prognostic and diagnose gastric cancer [3] . Therefore, it is very important to find accurate biomarkers for CG early detection, since most people don’t have any symptoms until it is already at an advanced stage [4]. The SLC16 family, part of the solute carrier (SLC) group, consists of transporters that shuttle monocarboxylates (MCTs), spanning from SLC16A1 to SLC16A14 (Halestrap and Denton 1974) . Specific members, such asMCT1 and MCT4, facilitate the transport of L-lactate crucial for the metabolic processes of cancer cells, with expression levels varying across different tumor types and stages (Ganapathy et al. 2009; Bosshart et al. 2021) . Overexpression of the metastasis-associated colon cancer-1 transcription factor in gastric cancer cells leads to an increase in MCT1 protein levels (Wang et al. 2017a) . MCTs 1–4 play a pivotal role in regulating intracellular pH levels by transporting substances like lactate (Halestrap 2013) . These transporters are known to be upregulated in various cancers, including breast, bone, colon, bladder, prostate, and renal cancers, correlating with poorer clinical outcomes [5] . RNA interference (RNAi) is a sequence-specific, post-transcriptional gene silenci

FAQs

What was the objective of reducing SLC16A13 in KATO2 gastric cancer cells?

To determine how lowering SLC16A13 expression affects survival, proliferation, apoptosis, and metastatic-related potential in KATO2 cells.

How were cell death and apoptosis evaluated after si-RNA transfection?

Cell death and apoptosis were measured using MTT and flow cytometry assays following SLC16A13 si-RNA transfection.

Did decreased SLC16A13 expression affect cancer cell migration?

No. The study reported that reduced SLC16A13 expression did not impact KATO2 cell migration as evaluated by the scratch test.

Sep 21, 2026

Uploaded by Theodore