CYP4X1 Expression Is Associated with Metastasis and Poor Prognosis in Patients with Colorectal Cancer

Sep 23, 2026

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Colorectal cancer mortality remains high despite targeted therapy and immunotherapy, making new biomarkers critical for prognosis and treatment tailoring. This study characterizes CYP4X1 in colorectal cancer using public TCGA-based resources (GENT2, UALCAN, GEPIA) and correlates expression with survival and clinicopathological features. CYP4X1 is elevated in tumors and links to advanced TNM stage, poor differentiation, deeper invasion, and lymph node metastasis. Functional assays show that siRNA-mediated CYP4X1 downregulation suppresses proliferation, invasion, migration, and colony formation in vitro, and reduces tumor growth in mouse models, supporting CYP4X1 as an independent prognostic marker and potential therapeutic target.

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CategoryHealthcare

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1. Introduction

Article CYP4X1 Expression Is Associated with Metastasis and Poor Prognosis in Patients with Colorectal Cancer Sooyoun Kim 1,2,†, Hakchun Kim 3,†, Inpyo Hong 1,2, Minho Lee 1,2, Hyeongjoo Kim 4, Hyoungjong Kwak 5, Chang-Jin Kim 5, Donghyun Kang 6, Taesung Ahn 6, Moojun Baek 6 and Dongjun Jeong 1,2, * Academic Editor: Carmine Stolfi Received: 6 January 2025 Revised: 25 January 2025 Accepted: 30 January 2025 Published: 21 February 2025 Citation: Kim, S.; Kim, H.; Hong, I.; Lee, M.; Kim, H.; Kwak, H.; Kim, C.-J.; Kang, D.; Ahn, T.; Baek, M.; et al. CYP4X1 Expression Is Associated with Metastasis and Poor Prognosis in Patients with Colorectal Cancer. Int. J. Mol. Sci. 2025, 26, 1867. [https://](https://)[ ](https://)[doi.org/10.3390/ijms26051867](doi.org/10.3390/ijms26051867) Correction Statement: This article has been republished with a minor change. The change does not affect the scientific content of the article and further details are available within the backmatter of the website version of this article. Copyright: © 2025 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ([https://creativecommons.org/](https://creativecommons.org/)[ ](https://creativecommons.org/)[licenses/by/4.0/](licenses/by/4.0/)) . 1 Department of Pathology, College of Medicine, Soonchunhyang University, 31 Soonchunhyang 6-gil, Dongnam-gu, Cheonan 31151, Chungcheongnam-do, Republic of Korea; [sooy.kim@sch.ac.kr](sooy.kim@sch.ac.kr) (S.K.); [hip0725@sch.ac.kr](hip0725@sch.ac.kr) (I.H.); [leemho00@sch.ac.kr](leemho00@sch.ac.kr) (M.L.) 2 BK21 Four Project, College of Medicine, Soonchunhyang University, Cheonan 31151, Chungcheongnam-do, Republic of Korea 3 Soonchunhyang Medical Science Research Institute, College of Medicine, Soonchunhyang University, 25 Bongjeong-ro, Dongnam-gu, Cheonan 31151, Chungcheongnam-do, Republic of Korea; [khc5467@sch.ac.kr](khc5467@sch.ac.kr) [4](4 R&D Center Pharmaceutical Laboratory)[ R&D Center Pharmaceutical Laboratory](4 R&D Center Pharmaceutical Laboratory), [Korean Drug Co](Korean Drug Co)., [Ltd](Ltd)., [34](34), [Nonhyeon-ro 28-gil](Nonhyeon-ro 28-gil), [Gangnam-gu](Gangnam-gu), Seoul 06300, Republic of Korea; [pmbaki2@gmail.com](pmbaki2@gmail.com) 5 Research Institute of Clinical Medicine, Woori Madi Medical Center, 111 Baekjedae-ro, Wansan-gu, Jeonju 55082, Jeollabuk-do, Republic of Korea; [k-h-jong@hanmail.net](k-h-jong@hanmail.net) (H.K.); [mountain48@hanmail.net](mountain48@hanmail.net) (C.-J.K.) 6 Department of Surgery, College of Medicine, Soonchunhyang University, 31 Soonchunhyang 6-gil, Dongnam-gu, Cheonan 31151, Chungcheongnam-do, Republic of Korea; [c100048@schmc.ac.kr](c100048@schmc.ac.kr) (D.K.); [eyetoeye@schmc.ac.kr](eyetoeye@schmc.ac.kr) (T.A.); [ssurge@sch.ac.kr](ssurge@sch.ac.kr) (M.B.) * [Correspondence: juny1024@sch.ac.kr](Correspondence: juny1024@sch.ac.kr); Tel.: +82-41-413-5049; Fax: +82-41-570-2546 † These authors contributed equally to this work. Abstract: Globally, the mortality rate of colorectal cancer (CRC) remains high. Despite the development of various treatments, such as targeted therapy and immunotherapy, colorectal cancer continues to be a serious health issue worldwide. Identifying new biomarkers is essential for improving prognosis and tailoring targeted therapies for CRC. This study aims to elucidate the role of CYP4X1 in CRC and its association with patient survival and clinicopathological parameters. Using TCGA databases like GENT2, UALCAN, and GEPIA, we analyzed CYP4X1 expression in CRC and normal tissues. Our analysis revealed a significant increase in CYP4X1 expression in CRC tissues compared to normal tissues. And CYP4X1 high expression was strongly associated with advanced TNM stage, poor tumor differentiation, deeper invasion, and lymph node metastasis. Kaplan–Meier analysis revealed that high CYP4X1 expressi

FAQs

What is the main purpose of the study on CYP4X1 in colorectal cancer?

To clarify the role of CYP4X1 in colorectal cancer and determine its association with patient survival and clinical-pathological parameters.

What relationship did the study find between CYP4X1 expression and patient outcomes?

High CYP4X1 expression was associated with shorter survival times and with worse clinicopathological features such as advanced TNM stage, poor differentiation, deeper invasion, and lymph node metastasis.

How did CYP4X1 knockdown affect colorectal cancer cell behavior in experiments?

siRNA-mediated CYP4X1 downregulation significantly inhibited cell proliferation, invasion, migration, and colony formation in vitro, and reduced tumor-forming ability in mice.

Sep 23, 2026

Uploaded by Asher