CRISPR/Cas9 - an overview of recent developments and applications in cancer research

Sep 21, 2026

Uploaded by Sage

Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated protein 9 (Cas9) is presented as a powerful gene-editing approach with broad potential for cancer research and therapy. The review consolidates work to date on CRISPR-Cas9, detailing core concepts, mechanisms of action, and its use to study cancer biology. It surveys applications from gene discovery to therapeutic design, while addressing challenges, limitations, and future directions.

LanguageEnglish

CategoryResearch & Report

FormatPDF

Pages16

Reading Time40 min

AccessFree to download

Abstract

Introduction

Cancer as a genome-related disease

Genome editing foundations and major milestones

Mechanisms leading to CRISPR-Cas9

’Invited Special Paper CRISPR/Cas9: an overview of recent developmentsand applications in cancer research Nandibala Devi Shamjetsabam, MSca , Rashmi Rana, PhDa,* , Priyanka Malik, MScb , Nirmal Kumar Ganguly, MDa Abstract Clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein 9 (Cas9) has risen as a potent gene editing method with vast potential across numerous domains, including its application in cancer research and therapy. This review article provides an extensive overview of the research that has been done so far on CRISPR-Cas9 with an emphasis on how it could be utilized in the treatment of cancer. The authors go into the underlying ideas behind CRISPR-Cas9, its mechanisms of action, and its application for the study of cancer biology. Furthermore, the authors investigate the various uses of CRISPR-Cas9 in cancer research, spanning from the discovery of genes and the disease to the creation of novel therapeutic approaches. The authors additionally discuss the challenges and limitations posed by CRISPR-Cas9 technology and offer insights into the potential applications and future directions of this cutting-edge ﬁeld of research. The article intends to consolidate the present understanding and stimulate more research into CRISPR-Cas9 ’s promise as a game-changing tool for cancer research and therapy. Keywords: cancer, CRISPR-Cas9, gene editing, cell therapy, homology-directed repair (HDR), nonhomologous end joining (NHEJ) Introduction Cancer is a global health issue affecting millions worldwide. Various treatments like surgery, radiation, chemotherapy, and combination therapies have been used to identify its underlying processes. However, alternative approaches have emerged due to postsurgery recurrence, resistance, and side effects. Cancer is a genome-related disease involving gene alterations and epigenome dysregulation[1] . Understanding the impact of genetic changes, cellular adaptations, and microenvironment changes on cancer treatment is crucial for advancement. High-throughput sequencing technology, speciﬁcally the clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein 9 (Cas9) system, has revolutionized cancer research by identifying genes linked to cancer initiation and progression, enhancing aDepartment of Biotechnology and Research, Sir Ganga Ram Hospital New Delhi and bDepartment of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University (GADVASU), Rampura Phul, Bathinda, Punjab, India Sponsorships or competing interests that maybe relevant to content are disclosed atthe end of this article. *Corresponding author. Address: Department of Biotechnology and Research, Associate Professor, GRIPMER, Sir Ganga Ram Hospital, New Delhi 110060, India. Tel.: +91 [114 225 1624. E-mail: rashmi.rana@sgrh.com](114 225 1624. E-mail: rashmi.rana@sgrh.com), [rana_aiims@yahoo.co.in](rana_aiims@yahoo.co.in) (R. Rana). Copyright © 2024 The Author(s). Published by Wolters Kluwer Health, Inc. This is an open access article distributed under the Creative Commons Attribution-ShareAlike License 4.0, which allows others to remix, tweak, and build upon the work, even for commercial purposes, as long as the author is credited and the new creations are licensed under the identical terms. International Journal of Surgery (2024) 110:6198–6213 Received 16 October 2023; Accepted 27 December 2023 Published online 21 February 2024 [http://dx.doi.org/10.1097/JS9.0000000000001081](http://dx.doi.org/10.1097/JS9.0000000000001081) HIGHLIGHTS • Clustered regularly interspaced short palindromic repeats (CRISPR)-CRISPR associated protein 9 (Cas9) technology allows precise genes modiﬁcations. • CRISPR-Cas9 enhances the effectiveness of cancer immunotherapies. • Enable in identifying the roles of various genes in cancer development and progression. • Advances the development of perso

FAQs

What is the main focus of the review article on CRISPR/Cas9?

The review provides an overview of recent developments and applications of CRISPR-Cas9 in cancer research, emphasizing how it can be used for cancer treatment and therapy.

How does CRISPR-Cas9 contribute to studying cancer biology?

It supports discovering gene roles in cancer initiation and progression and enables functional genomics studies through precise, efficient genome editing.

What are the key challenges and limitations discussed for CRISPR-Cas9?

The article discusses challenges related to CRISPR-Cas9 technology and limitations that affect its use, and it highlights directions for future research and applications.

Sep 21, 2026

Uploaded by Sage