Could be FOXO3a, miR-96-5p and miR-182-5p useful for Brazilian women with luminal A and triple negative breast cancers prognosis and target therapy - Clinical Highlights - Article

Q: Which miRNAs and target does the study focus on?

The study focuses on miR-96-5p and miR-182-5p as negative regulators of FOXO3A, examining their expression and relationship with FOXO3A in Brazilian patients’ breast cancer samples.

Q: How were miRNA expression levels measured and compared?

Expression profiles were measured using quantitative real-time PCR, comparing Luminal A and Triple-Negative tumor tissues with normal tissue.

Q: What is the reported relationship between miRNA expression and FOXO3a protein detection?

Higher miR-96-5p and miR-182-5p expression (FR ≥ 4) was associated with reduced FOXO3a protein detection across tumors, with the most prominent miRNA expression and FOXO3a suppression observed in Triple-Negative samples.

Sep 24, 2026

Uploaded by dhado

FOXO3a dysregulation is frequently implicated in tumorigenesis, and inhibition can occur through multiple molecular mechanisms, including miRNA-mediated post-transcriptional suppression. This study assessed expression profiles of relevant miRNAs in Luminal A and Triple-Negative breast cancers from Brazilian patients using quantitative real-time PCR, and evaluated their potential prognostic value. miR-96-5p and miR-182-5p showed differential expression in tumors versus normal tissue and were linked with reduced FOXO3a protein detection, especially in Triple-Negative samples, supporting alternative target therapy perspectives.

LanguageEnglish

CategoryHealthcare

FormatPDF

Pages10

Reading Time25 min

AccessFree to download

Introduction

Breast cancer incidence and prognosis context

Molecular mechanisms in mammary carcinogenesis

Gene-expression based classification

Luminal A features

Triple-Negative features

Clinics 78 (2023) 100155 journal homepage: [https://www.journals.elsevier.com/clinics](https://www.journals.elsevier.com/clinics) | Original articles | | | | --- | --- | --- | | Could be FOXO3a, miR-96-5p and miR-182-5p useful for Brazilian women with luminal A and triple negative breast cancers prognosis and target therapy? | | | | Daniele Carvalho Calvano Mendes a, Carlos Marino Cabral Calvano Filho a,b, Natlia Garcia a, Marcos Desidrio Ricci a, Jos Maria Soares Jniora, Katia Candido Carvalho a, *, Edmund Chada Baracat a
a Laboratrio de Ginecologia Estrutural e Molecular (LIM 58), Disciplina de Ginecologia, Departamento de Obstetrícia e Ginecologia, Hospital das Clínicas da Faculdade de Medicina da Universidade de S~ao Paulo, HCFMUSP, S~ao Paulo, SP, Brazil
b Setor de Mastologia, Disciplina de Ginecologia, Universidade de Brasília, Brasília, DF, Brazil | | | | H I G H L I G H T S | | | | 􀀁 miR-96-5p and 182-5p are associated with FOXO3a dysregulation and cancer.
􀀁 FOXO3a and miRNAs represent molecular markers in Brazilian breast cancer patients. | | | | A R T I C L E I N F O
Keywords: Breast cancer miRNAs
FOXO3aqRT-PCR
IHC | A B S T R A C T
FOXO3a dysregulation is frequently implicated in tumorigenesis, and its inhibition can occur by several molecular mechanisms. Among these, post-transcriptional suppression by miRNAs has been associated with various cancers initiation. Here, we assessed the expression proﬁles of the most relevant miRNAs for breast tumorigenesis, using Luminal A (LA) and Triple-Negative (TN) breast cancer from Brazilian patients, by the quantitative real time-PCR method. Their potential prognostic role for the patients was also evaluated. We identiﬁed the miRNAs miR-96-5p and miR-182-5p, de-scribed as negative regulators of FOXO3A, with differential expression both in LA and TN tumors when compared to normal tissue. The miR-96-5p and miR-182-5p miRNAs were upregulated in LA (7.82 times, p < 0.005; 6.12 times, p < 0.005, respectively) and TN breast cancer samples (9.42 times, p < 0.0001; 8.51 times, p < 0.0001) compared to normal tissues. The samples with higher miR-96-5p and miR-182-5p expression (FR ≥ 4) were submitted for FOXO3a immunostaining. Reduced protein detection was observed in all of the tumors compared to normal tissues. The most prominent miRNA expression and FOXO3a protein suppression were observed in TN samples (p < 0.001), indicating the relevant role of these molecules in this tumor biology and clinical behavior. Our results corroborate the literature regarding to the relevance of FOXO3a in the breast cancer, and they open new perspectives for alternative target therapy options for Brazilian patients expressing both FOXO3a and its regulatory miRNAs. | | Introduction Breast cancer is the most frequent malignant tumor among women, with the exception of non-melanoma skin cancers. It corresponds to 24.2% of all female malignant tumors, and its incidence rate is 46.3/ 100,000.1 In Brazil in 2018, there were 17,572 deaths due to breast cancer in women, which corresponds to 16.4% of cancer deaths in the female population.2 Though it is considered to have a relatively good prognosis, breast cancer mortality rates remain high in the country. The mean ﬁve-year survival in developed countries has increased slightly, reaching 85%. However, in developing countries, the survival is approximately 60% .3 The main landmarks of mammary carcinogenesis are related to proliferation, differentiation, and apoptosis. The molecular interactions involved in these processes are not fully understood yet. In fact, the network of mechanistic and physical interactions among genes, proteins, and metabolites remains largely unexplored due to the immense complexity of the connections among the various pathways regulating these cellular processes.4 Studies that have applied theoretical approaches to explore genes directly involved in the pathogenesis of breast cancer have revealed

FAQs

Which miRNAs and target does the study focus on?

The study focuses on miR-96-5p and miR-182-5p as negative regulators of FOXO3A, examining their expression and relationship with FOXO3A in Brazilian patients’ breast cancer samples.

How were miRNA expression levels measured and compared?

Expression profiles were measured using quantitative real-time PCR, comparing Luminal A and Triple-Negative tumor tissues with normal tissue.

What is the reported relationship between miRNA expression and FOXO3a protein detection?

Higher miR-96-5p and miR-182-5p expression (FR ≥ 4) was associated with reduced FOXO3a protein detection across tumors, with the most prominent miRNA expression and FOXO3a suppression observed in Triple-Negative samples.

Sep 24, 2026

Uploaded by dhado