Circ_ 0001387 regulates SKA2 to accelerate breast cancer progression through miR-136-5p

Sep 24, 2026

Uploaded by Rhys

Background: Growing evidence identifies circular RNAs as key regulators in cancer. This study investigated circ_0001387 function in breast cancer and its potential regulatory network with miR-136-5p and spindle and kinetochore-associated protein 2 (SKA2). Methods: circ_0001387, miR-136-5p, and SKA2 levels were quantified by qRT-PCR. Cell proliferation, apoptosis, migration, and invasion were assessed using clone formation, 5-ethynyl-20-deoxyuridine assays, flow cytometry, and transwell assays, with mechanistic validation of miR-136-5p interactions and in vivo testing using a xenograft mouse model. Results: circ_0001387 and SKA2 were upregulated while miR-136-5p was low in breast cancer. circ_0001387 knockdown suppressed malignant behaviors in vitro and in vivo via miR-136-5p, while SKA2 restored these suppressive effects. Conclusion: circ_0001387 promotes breast cancer progression through the miR-136-5p/SKA2 axis.

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CategoryHealthcare

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DOI: 10.1111/1759-7714.14916 ORIGINA L ARTICLE Circ_ 0001387 regulates SKA2 to accelerate breast cancer progression through miR-136-5p Youyi Xiong | Lin Li | Nan Wang | Fang Wang | Zhuo Chen | Luhong Han | Shan Jiang | Yuanting Gu Department of Breast Surgery, The First Affiliated Hospital of Zhengzhou University, Zhengzhou City, China Correspondence Yuanting Gu, Department of Breast Surgery, The First Affiliated Hospital of Zhengzhou University, No. 1 Jianshe East Road, Erqi District, Zhengzhou City, Henan Province, China. Email: [guytbr@126.com](guytbr@126.com) Funding information Henan Province Medical Science and Technology Research Program Joint Construction Project, Grant/Award Number: LHGJ20200333 Abstract Background: Growing evidence has revealed the critical regulatory role for circular RNAs (circRNAs) in cancer. This study aimed to explore the function of circ_0001387 in breast cancer (BC) . Methods: Circ_ 0001387, miR-136-5p, and spindle and kinetochore-associated protein 2 (SKA2) levels were analyzed by quantitative real-time polymerase chain reaction. Clone formation and 5-ethynyl-20-deoxyuridine assays were used to analyze cell proliferation. Cell apoptosis and cell migration and invasion abilities were analyzed using flow cytometry or transwell assay. Mechanism assay was used to confirm the association between miR-136-5p and circ_ 0001387 or SKA2 . The effect ofcirc_ 0001387 on tumor growth in vivo was analyzed by the xenograft mice model. Results: Circ_ 0001387 and SKA2 were expressed at high levels, whereas miR-136-5p was lowly expressed in BC tissues and cells. Meanwhile, the downregulation ofcirc_ 0001387 restrained BC cell progression in vitro and in vivo. Circ_ 0001387 competitively bound to miR-136-5p to regulate BC cell malignant behaviors. SKA2 was targeted by miR-136-5p, and SKA2 reinstated the suppressive effect of miR-136-5pupregulation in BC cells. Conclusion: Our study indicated that circ_ 0001387 contributed to BC cell progression through miR-136-5p/SKA2 axis. KEYW ORD S breast cancer, circ_ 0001387, miR-136-5p, SKA2 INTRODUCTION The latest cancer statistics reported in 2020 showed that there was more than 2 million new breast cancer (BC) patients worldwide, surpassing lung cancer.1 The deaths of BC are as high as 680 000, making it the most reason for cancer death among women.1 The combination of surgical resection and radiotherapy is the major treatment method for BC, which prolonged the survival of BC patients,2 and the 5-year survival rate for non-metastatic BC is as high as 80%.3 However, BC patients still have a high likelihood of metastasis even after surgical resection and other treatment measures,4 and their 5-year survival rates drops to 25%.5 Therefore, an in- depth study of BC pathogenesis and the search for promising molecular targets to enhance the effectiveness of BC treatment, as well as to alleviate the drug resistance phenomenon arising during chemotherapy, are essential to improve the survival of BC patients. Derived from exon-or intron-specific splicing of genes to form circular structures, circular RNAs (circRNAs) can be detected in all types of organisms.6 Numberous references documented the role of circRNAs in various diseases.7 CircRNAs are potential molecular markers for various diseases owing to their high stability.8 In BC, a substantial quantity of dysregulated circRNAs were also identified. Cao et al.9 disclosed that circRNF20 was notably elevated in BC and closely This is an open access article under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. © 2023 The Authors. Thoracic Cancer published by China Lung Oncology Group and John Wiley & Sons Australia, Ltd. associated with the severity of breast cancer. Zeng et al.10 also reve

FAQs

What was the main goal of the study on circ_0001387 in breast cancer?

To determine how circ_0001387 influences breast cancer progression and whether it regulates the miR-136-5p/SKA2 pathway.

How were circ_0001387, miR-136-5p, and SKA2 levels measured?

Levels were analyzed using quantitative real-time polymerase chain reaction (qRT-PCR).

What key biological effects did circ_0001387 downregulation have?

Downregulation of circ_0001387 restrained breast cancer cell progression in vitro and in vivo.

How did miR-136-5p and SKA2 contribute to the mechanism?

circ_0001387 competitively bound miR-136-5p; miR-136-5p targeted SKA2, and SKA2 could reinstate the suppressive effect caused by miR-136-5p upregulation.

Sep 24, 2026

Uploaded by Rhys