CD47 expression in non-small cell lung cancer and its relationship with tumor-associated macrophage infiltration - research article

Q: What was the study objective regarding CD47 in NSCLC?

To investigate CD47 expression in non-small cell lung cancer and determine its relationship with tumor-associated macrophage infiltration.

Q: How was CD47 expression assessed in tumor and adjacent tissues?

Immunohistochemistry and confocal microscopy were used to evaluate CD47 expression, while immunofluorescence examined CD47 on tumor-infiltrating T lymphocytes.

Q: What did the study find about CD47 and patient prognosis?

CD47 positivity in tumor tissues was higher than in adjacent tissues, and CD47 expression correlated with lymph node metastasis, clinical stage, differentiation, and poorer survival.

Sep 21, 2026

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Non-small cell lung cancer (NSCLC) remains highly lethal, with many patients diagnosed after the optimal surgical window. Survival improvement depends on identifying reliable prognostic markers, and immune checkpoint molecules may support better risk stratification. This study examined CD47 expression in NSCLC and evaluated how it relates to tumor-associated macrophage (TAM) infiltration. Using retrospective patient data and immunologic assays, the work assessed associations with clinicopathologic features and survival outcomes.

LanguageEnglish

CategoryResearch & Report

FormatPDF

Pages18

Reading Time45 min

AccessFree to download

Abstract

Background

Objective

Methods

Results

Conclusion

1. Introduction

PLOS ONE OPEN ACCESS Citation: Zhang H, Liu S, Zhang J, Wang Y (2024) CD47 expression in non-small cell lung cancer and its relationship with tumor-associated macrophage infiltration. PLoS ONE 19(12): e0314228 . [https://](https://)[ ](https://)[doi.org/10.1371/journal.pone.0314228](doi.org/10.1371/journal.pone.0314228) [Editor:](Editor: Jeffrey S. Isenberg)[ Jeffrey S. Isenberg](Editor: Jeffrey S. Isenberg), [City of](City of) Hope National Medical Center, UNITED STATES OF AMERICA Received: August 16, 2024 Accepted: November 6, 2024 Published: December 9, 2024 Copyright: © 2024 Zhang et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability Statement: All relevant data are within the paper and its Supporting Information files. Funding: The author(s) received no specific funding for this work. Competing interests: The authors have declared that no competing interests exist. RESEARCH ARTICLE CD47 expression in non-small cell lung cancer and its relationship with tumor-associated macrophage infiltration Hefeng Zhang, Shihu Liu, Jinzi Zhang, Yongjie Wang * Department of Thoracic Surgery, The Affiliated Hospital of Qingdao University, Qingdao, Shandong, China * [wangyongjie@qdu.edu.cn](wangyongjie@qdu.edu.cn) Abstract Background Non-small cell lung cancer (NSCLC) has a high incidence, with most patients diagnosed beyond the optimal surgical window. Improving survival rates is critical to reducing lung cancer mortality, and identifying immune checkpoints is vital for prognosis stratification. Objective To investigate the expression of CD47 in NSCLC and its relationship with tumor-associated macrophage infiltration. Methods A retrospective analysis was conducted on 50 NSCLC patients treated between January 2014 and June 2018. Immunohistochemistry and confocal microscopy assessed CD47 expression in tumor and adjacent tissues, while immunofluorescence evaluated CD47 on tumor-infiltrating T lymphocytes. Kaplan-Meier survival analysis and Cox regression identified prognostic factors, and PLA technology examined CD47’s interaction with VEGFR and CD36 . Results CD47 positivity in tumor tissues (52%) was significantly higher than in adjacent tissues (20%) (P<0.001), with expression localized to the cell membrane. CD47 expression correlated with lymph node metastasis, clinical stage, and differentiation (P<0.05) and was identified as an independent risk factor for poor prognosis. TAM infiltration was greater in CD47-positive patients and correlated with shorter survival (P<0.05) . PLA showed stronger CD47 +VEGFR interactions than CD47+CD36 . Conclusion CD47 positivity correlates with poor prognosis and increased TAM infiltration, highlighting its potential as a prognostic biomarker and therapeutic target in NSCLC. 1. Introduction Lung cancer is currently one of the cancer types with the highest incidence and mortality rates worldwide, which seriously affects human health and life [ 1] . According to the recent statistics from the American Cancer Society (ACS), 1,958,310 new cancer cases and 609,820 cancer deaths are expected to occur in the United States in 2023, of which 238,340 new lung cancer cases are predicted, accounting for 12. 17% of the total number ofnew cancer cases. The number of new cases of lung cancer is predicted to be 238,340, accounting for 12. 17% of all new cases of cancer, and the number of deaths due to lung cancer in the coming year is estimated to be 127,070, accounting for 20 .83% of the total number of cancer deaths. Non-small cell lung cancer (NSCLC) accounts for more than 80% of all lung cancers [2,3] . How to reduce the mortality ofNSCLC patients is a problem that cannot be ignored, and effective prognostic indicators are important for the subsequent individua

FAQs

What was the study objective regarding CD47 in NSCLC?

To investigate CD47 expression in non-small cell lung cancer and determine its relationship with tumor-associated macrophage infiltration.

How was CD47 expression assessed in tumor and adjacent tissues?

Immunohistochemistry and confocal microscopy were used to evaluate CD47 expression, while immunofluorescence examined CD47 on tumor-infiltrating T lymphocytes.

What did the study find about CD47 and patient prognosis?

CD47 positivity in tumor tissues was higher than in adjacent tissues, and CD47 expression correlated with lymph node metastasis, clinical stage, differentiation, and poorer survival.

Sep 21, 2026

Uploaded by Evangeline