B4GALT5 a sialylation-related genes associated with patient prognosis and immune microenvironment in ovarian cancer and pan-cancer

Sep 21, 2026

Uploaded by Theodore

Ovarian cancer remains difficult to treat, and abnormal sialylation contributes to tumor progression, metastasis, immune evasion, angiogenesis, and resistance. The study evaluates B4GALT5, a sialylation-associated gene, for links to clinicopathological features, patient prognosis, and the tumor immune microenvironment in ovarian cancer and across cancers. Integrated bioinformatics analyses combined with immunohistochemistry and functional assays assess B4GALT5 expression, growth effects, and pathway enrichment.

LanguageEnglish

CategoryResearch & Report

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Pages16

Reading Time40 min

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Abstract

Introduction

Materials and methods

Results

Conclusion

Keywords

Wu et al. Journal of Ovarian Research (2024) 17:176 Journal of Ovarian Research [https://doi.org/10.1186/s13048-024-01503-3](https://doi.org/10.1186/s13048-024-01503-3) RESEARCH Open Access B4GALT5 a sialylation-related genes associated with patient prognosis and immune microenvironment in ovarian cancer and pan-cancer Di Wu 1, Li-yuan Sun 1, Xin-yu Chang 1 and Guang-mei Zhang 1* Abstract Background Ovarian cancer (OC) is the predominant primary tumor in the human reproductive system. Abnormal sialylation has a significant impact on tumor development, metastasis, immune evasion, angiogenesis, and treatment resistance. B4GALT5, a gene associated with sialylation, plays a crucial role in ovarian cancer, and may potentially affect clinicopathological characteristics and prognosis. Methods We conducted a comprehensive search across TIMER, GEPIA2, GeneMANIA, and Metascape to obtain transcription profiling data of ovarian cancer from The Cancer Genome Atlas (TCGA) . The expression of B4GALT5 was test by immunohistochemistry. To investigate the impact of B4GALT5 on growth and programmed cell death in OC cells, we performed transwell assays and western blots. Results The presence of B4GALT5 was strongly associated with an unfavorable outcome in OC. B4GALT5 significantly promoted the proliferation of OC cells. Upon analyzing gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), it was discovered that B4GALT5 played a crucial role in the extracellular matrix, particularly in collagen-containing structures, and exhibited correlations with ECM-receptor interactions, transcriptional dysregulation in cancer, as well as the interleukin-1 receptor signaling pathway. Furthermore, there is a clear link between B4GALT5 and the tumor immune microenvironment in OC. Moreover, B4GALT5 exhibits favorable expression levels across various types of cancers, including CHOL, KIRC, STAD and UCES. Conclusion In conclusion, it is widely believed that B4GALT5 plays a pivotal role in the growth and progression of OC, with its heightened expression serving as an indicator of unfavorable outcomes. Moreover, B4GALT5 actively participates in shaping the cancer immune microenvironment within OC. This investigation has the potential to contribute significantly to a deeper understanding of the substantial involvement of B4GALT5 in human malignancies, particularly OCs. Keywords B4GALT5, Ovarian cancer, Sialylation, Tumor microenvironment *Correspondence: Guang-mei Zhang [guangmeizhang@126.com](guangmeizhang@126.com) 1Department of Obstetrics and Gynecology, The First Affiliated Hospital of Harbin Medical University, Harbin, Heilongjiang Province 150001, China © The Author(s) 2024. Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit [http://](http://)[ ](http://)[creativecommons.org/l](creativecommons.org/l)icenses/by-nc-nd/4.0/. Wu et al. Journal of Ovarian Research (2024) 17:176 Introduction Ovarian cancer is often diagnosed at an advanced stage, rendering it the most fatal among all gynecological cancers [1]. De

FAQs

What is the biological role of B4GALT5 in ovarian cancer according to the study?

B4GALT5 is associated with unfavorable outcomes and promotes ovarian cancer cell proliferation. It also participates in shaping the tumor immune microenvironment.

Which analytical tools and experiments were used to study B4GALT5?

The study uses TIMER, GEPIA2, GeneMANIA, and Metascape for transcription profiling, performs immunohistochemistry to test B4GALT5 expression, and uses transwell assays and western blots to examine growth and programmed cell death effects.

What pathways and biological processes are enriched for B4GALT5?

Gene ontology and KEGG analyses indicate a role in extracellular matrix processes, especially collagen-containing structures, ECM-receptor interactions, transcriptional dysregulation, and the interleukin-1 receptor signaling pathway.

Sep 21, 2026

Uploaded by Theodore